################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AclI AcsI AfaI AflII AgeI AhdI AlwI AlwNI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsgI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI Bst1107I BstAPI BstBI BstF5I BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraIII DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI MamI MboI MfeI MflI MluI MseI MslI MunI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SalI Sau3AI SbfI ScaI SfcI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 667 GTCTAC GT^[AC][GT]AC AccIII 1104 TCCGGA T^CCGGA AflIII 218 ACGTGT A^C[GA][CT]GT Alw44I 873 GTGCAC G^TGCAC ApaLI 873 GTGCAC G^TGCAC AspHI 873 GTGCAC G[AT]GC[AT]^C BbsI 1073 GTCTTC GAAGAC(2/6) BpmI 202 CTCCAG CTGGAG(16/14) BpuAI 1073 GTCTTC GAAGAC(2/6) BsaAI 610 TACGTG [CT]AC^GT[GA] BsaI 883 GGTCTC GGTCTC(1/5) BsaWI 1104 TCCGGA [AT]^CCGG[AT] BseAI 1104 TCCGGA T^CCGGA BsiHKAI 873 GTGCAC G[AT]GC[AT]^C BsmBI 630 CGTCTC CGTCTC(1/5) BspEI 1104 TCCGGA T^CCGGA BspHI 518 TCATGA T^CATGA BspMI 164 ACCTGC ACCTGC(4/8) BsrBI 1027 CCGCTC CCGCTC(-3/-3) BstEII 444 GGTCACC G^GT[ACGT]ACC BstPI 444 GGTCACC G^GT[ACGT]ACC EcoO65I 444 GGTCACC G^GT[ACGT]ACC HgaI 547 GACGC GACGC(5/10) KspI 497 CCGCGG CCGC^GG MroI 1104 TCCGGA T^CCGGA NcoI 37 CCATGG C^CATGG NspI 602 ACATGC [GA]CATG^[CT] PflMI 182 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 518 TCATGA T^CATGA SacII 497 CCGCGG CCGC^GG SapI 660 GCTCTTC GCTCTTC(1/4) SexAI 248 ACCTGGT A^CC[AT]GGT SmaI 969 CCCGGG CCC^GGG Tsp509I 1012 AATT ^AATT Van91I 182 CCATCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 91 CCAGGGGTTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 969 CCCGGG C^CCGGG XmaI 969 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### Aor51HI 452 AGCGCT AGC^GCT 560 AGCGCT BseRI 624 CTCCTC GAGGAG(10/8) 742 GAGGAG BssHII 838 GCGCGC G^CGCGC 942 GCGCGC BssSI 16 CACGAG CACGAG(-5/-1) 652 CTCGTG Eco47III 452 AGCGCT AGC^GCT 560 AGCGCT HphI 252 GGTGA GGTGA(8/7) 446 TCACC PvuII 467 CAGCTG CAG^CTG 700 CAGCTG SfaNI 657 GATGC GCATC(5/9) 371 GCATC SgrAI 275 CGCCGGCG C[GA]^CCGG[CT]G 752 CGCCGGCG TaqI 374 TCGA T^CGA 1199 TCGA TthHB8I 374 TCGA T^CGA 1199 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI KasI Ksp632I MaeI MaeII MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NlaIII NlaIV PleI Sau96I ScrFI SfoI StyI TseI Tsp45I TspRI