(Circular) (Six-base) MAPSORT of: pFN21AE9571.seq Check: 8437 from: 1 to: 5929 pFN21AE9571 5929 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2673 3272 1937 Size: 736 599 4594 Fragments arranged by size: 4594 736 599 Acc65I G'GTAC_C Cuts at: 3250 3250 Size: 5929 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3992 5376 5644 1632 Size: 2360 1384 268 1917 Fragments arranged by size: 2360 1917 1384 268 AfeI AGC'GCT Cuts at: 2459 2567 2459 Size: 108 5821 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 2223 4828 5805 1203 Size: 1020 2605 977 1327 Fragments arranged by size: 2605 1327 1020 977 AgeI A'CCGG_T Cuts at: 4760 4760 Size: 5929 AhdI GACnn_n'nnGTC Cuts at: 4658 4658 Size: 5929 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5929 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5929 AlwNI CAG_nnn'CTG Cuts at: 3672 5244 3672 Size: 1572 4357 ApaI G_GGCC'C Cuts at: 2131 2875 2963 2981 2131 Size: 744 88 18 5079 Fragments arranged by size: 5079 744 88 18 ApaLI G'TGCA_C Cuts at: 2878 3986 5142 2878 Size: 1108 1156 3665 Fragments arranged by size: 3665 1156 1108 ApoI r'AATT_y Cuts at: 1493 3238 3464 3616 5725 1493 Size: 1745 226 152 2109 1697 Fragments arranged by size: 2109 1745 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 2744 2974 3148 3254 1870 Size: 73 801 230 174 106 4545 Fragments arranged by size: 4545 801 230 174 106 73 BamHI G'GATC_C Cuts at: 3259 3626 3259 Size: 367 5562 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2275 2321 2761 2818 Size: 325 217 627 278 210 46 440 57 Cuts at: 2818 2900 3250 4705 618 Size: 82 350 1455 1842 Fragments arranged by size: 1842 1455 627 440 350 325 278 217 210 82 57 46 BanII G_rGCy'C Cuts at: 1391 2131 2875 2963 2981 3117 3248 1391 Size: 740 744 88 18 136 131 4072 Fragments arranged by size: 4072 744 740 136 131 88 18 BbeI G_GCGC'C Cuts at: 2069 2325 2765 2069 Size: 256 440 5233 Fragments arranged by size: 5233 440 256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3071 928 Size: 971 1172 3786 Fragments arranged by size: 3786 1172 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4120 4154 1903 Size: 34 2183 34 3678 Fragments arranged by size: 3678 2183 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3821 5031 3821 Size: 1210 4719 BclI T'GATC_A Cuts at: 1548 1548 Size: 5929 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 2294 3093 Size: 107 122 71 631 605 463 159 799 Cuts at: 3093 3134 4540 136 Size: 41 1406 1525 Fragments arranged by size: 1525 1406 799 631 605 463 159 122 107 71 41 BglII A'GATC_T Cuts at: 5924 5924 Size: 5929 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 2131 2823 2875 2882 2903 2963 2981 3990 Size: 341 692 52 7 21 60 18 1009 Cuts at: 3990 5146 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1009 692 341 60 52 21 18 7 BmgBI CAC'GTC Cuts at: 1318 2356 2799 1318 Size: 1038 443 4448 Fragments arranged by size: 4448 1038 443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4618 442 Size: 416 53 83 399 24 2243 958 1753 Fragments arranged by size: 2243 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5929 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2047 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 4589 1239 Size: 953 2397 2579 Fragments arranged by size: 2579 2397 953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4054 4919 5217 4054 Size: 865 298 4766 Fragments arranged by size: 4766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2894 4592 882 Size: 617 1395 1698 2219 Fragments arranged by size: 2219 1698 1395 617 BsaAI yAC'GTr Cuts at: 493 1204 2617 3720 493 Size: 711 1413 1103 2702 Fragments arranged by size: 2702 1413 1103 711 BsaBI GATnn'nnATC Cuts at: 5923 5923 Size: 5929 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2322 2553 2762 Size: 53 83 186 936 533 256 231 209 Cuts at: 2762 3736 4118 4753 275 Size: 974 382 635 1451 Fragments arranged by size: 1451 974 936 635 533 382 256 231 209 186 83 53 BsaWI w'CCGG_w Cuts at: 3109 4362 4760 5034 5181 5592 3109 Size: 1253 398 274 147 411 3446 Fragments arranged by size: 3446 1253 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2788 2818 3213 3243 1178 Size: 30 165 30 1385 30 395 30 3864 Fragments arranged by size: 3864 1385 395 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2620 2762 1186 Size: 1434 142 4353 Fragments arranged by size: 4353 1434 142 BseYI C'CCAG_C Cuts at: 2728 3175 5132 5628 2728 Size: 447 1957 496 3029 Fragments arranged by size: 3029 1957 496 447 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2502 2833 3097 3379 4140 5168 Size: 275 186 501 331 264 282 761 1028 Cuts at: 5168 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 501 331 282 275 264 186 BsiHKAI G_wGCw'C Cuts at: 2882 3990 4075 5146 2882 Size: 1108 85 1071 3665 Fragments arranged by size: 3665 1108 1071 85 BsmI GAATG_Cn' Cuts at: 3440 3533 3440 Size: 93 5836 BsmBI CGTCTCn'nnnn_ Cuts at: 2641 2641 Size: 5929 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2823 2875 2882 2903 2963 2981 Size: 399 341 692 52 7 21 60 18 Cuts at: 2981 3117 3248 3990 4075 5146 1391 Size: 136 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 399 341 136 131 85 60 52 21 18 7 BspEI T'CCGG_A Cuts at: 3109 5592 3109 Size: 2483 3446 BspHI T'CATG_A Cuts at: 2523 3818 5548 2523 Size: 1295 1730 2904 Fragments arranged by size: 2904 1730 1295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 3284 844 Size: 1334 1106 3489 Fragments arranged by size: 3489 1334 1106 BsrBI CCG'CTC Cuts at: 1394 1823 3034 3816 1394 Size: 429 1211 782 3507 Fragments arranged by size: 3507 1211 782 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4424 4598 65 Size: 1183 3176 174 1396 Fragments arranged by size: 3176 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2281 2758 2832 4573 4760 1700 Size: 110 471 477 74 1741 187 2869 Fragments arranged by size: 2869 1741 477 471 187 110 74 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5929 BssHII G'CGCG_C Cuts at: 1925 2843 2947 1925 Size: 918 104 4907 Fragments arranged by size: 4907 918 104 BssSI C'ACGA_G Cuts at: 2021 2657 3989 5001 2021 Size: 636 1332 1012 2949 Fragments arranged by size: 2949 1332 1012 636 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5929 BstBI TT'CG_AA Cuts at: 4733 4733 Size: 5929 BstEII G'GTnAC_C Cuts at: 1184 2449 1184 Size: 1265 4664 BstYI r'GATC_y Cuts at: 1972 3259 3626 4011 4028 5480 5566 5578 5924 Size: 1287 367 385 17 1452 86 12 346 Cuts at: 5924 1972 Size: 1977 Fragments arranged by size: 1977 1452 1287 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2012 2042 2105 2157 2400 2502 Size: 552 939 8 30 63 52 243 102 Cuts at: 2502 513 Size: 3940 Fragments arranged by size: 3940 939 552 243 102 63 52 30 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2173 487 Size: 683 56 947 4243 Fragments arranged by size: 4243 947 683 56 BtsI GCAGTG_nn' Cuts at: 3445 4239 4259 3445 Size: 794 20 5115 Fragments arranged by size: 5115 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2068 2103 529 Size: 35 1504 35 4355 Fragments arranged by size: 4355 1504 35 35 DraI TTT'AAA Cuts at: 3233 3233 Size: 5929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2151 2184 Size: 54 1425 50 275 18 215 106 33 Cuts at: 2184 2241 2286 2305 2312 2397 2499 2830 2934 Size: 57 45 19 7 85 102 331 104 Cuts at: 2934 3094 3376 4265 5894 8 Size: 160 282 889 1629 43 Fragments arranged by size: 1629 1425 889 331 282 275 215 160 106 104 102 85 57 54 50 45 43 33 19 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2499 2830 3094 3376 1537 Size: 275 687 331 264 282 4090 Fragments arranged by size: 4090 687 331 282 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 2658 2672 3146 3859 1353 Size: 1305 14 474 713 3423 Fragments arranged by size: 3423 1305 713 474 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2730 2745 2796 3062 4516 4890 Size: 1064 138 1141 15 51 266 1454 374 Cuts at: 4890 5036 387 Size: 146 1280 Fragments arranged by size: 1454 1280 1141 1064 374 266 146 138 51 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 3992 4589 5376 5644 1239 Size: 393 560 1800 597 787 268 1524 Fragments arranged by size: 1800 1524 787 597 560 393 268 EcoO109I rG'GnC_Cy Cuts at: 2127 2128 2872 2959 2960 2127 Size: 1 744 87 1 5096 Fragments arranged by size: 5096 744 87 1 1 EcoRI G'AATT_C Cuts at: 3238 3616 3238 Size: 378 5551 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 2069 2141 2300 2325 2461 2569 2765 3069 Size: 391 72 159 25 136 108 196 304 Cuts at: 3069 5076 1678 Size: 2007 2531 Fragments arranged by size: 2531 2007 391 304 196 159 136 108 72 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2788 2820 4570 4602 4644 Size: 32 135 32 1216 32 1750 32 42 Cuts at: 4644 4676 1373 Size: 32 2626 Fragments arranged by size: 2626 1750 1216 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3273 3519 669 Size: 988 281 1335 246 3079 Fragments arranged by size: 3079 1335 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KasI G'GCGC_C Cuts at: 2065 2321 2761 2065 Size: 256 440 5233 Fragments arranged by size: 5233 440 256 KpnI G_GTAC'C Cuts at: 3254 3254 Size: 5929 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2601 2628 5043 5227 1305 Size: 1296 27 2415 184 2007 Fragments arranged by size: 2415 2007 1296 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2153 2186 2307 2936 10 Size: 54 1425 343 321 33 121 629 3003 Fragments arranged by size: 3003 1425 629 343 321 121 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 3887 4246 4405 518 Size: 648 2721 359 159 2042 Fragments arranged by size: 2721 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2431 2474 2504 2707 3090 3345 4022 5170 Size: 825 43 30 203 383 255 677 1148 Cuts at: 5170 5415 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 825 677 383 255 245 203 43 30 NaeI GCC'GGC Cuts at: 2283 2760 2834 2283 Size: 477 74 5378 Fragments arranged by size: 5378 477 74 NarI GG'CG_CC Cuts at: 2066 2322 2762 2066 Size: 256 440 5233 Fragments arranged by size: 5233 440 256 NcoI C'CATG_G Cuts at: 513 1065 2004 2042 513 Size: 552 939 38 4400 Fragments arranged by size: 4400 939 552 38 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NgoMIV G'CCGG_C Cuts at: 2281 2758 2832 2281 Size: 477 74 5378 Fragments arranged by size: 5378 477 74 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5929 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 2611 3287 4832 1132 Size: 97 1382 676 1545 2229 Fragments arranged by size: 2229 1545 1382 676 97 PasI CC'CwG_GG Cuts at: 1348 2953 1348 Size: 1605 4324 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 2193 1108 Size: 1085 4844 PfoI T'CCnGG_A Cuts at: 2859 2859 Size: 5929 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3998 4030 5511 5543 3998 Size: 32 1481 32 4384 Fragments arranged by size: 4384 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5929 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PspOMI G'GGCC_C Cuts at: 2127 2871 2959 2977 2127 Size: 744 88 18 5079 Fragments arranged by size: 5079 744 88 18 PstI C_TGCA'G Cuts at: 830 3281 830 Size: 2451 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5929 PvuII CAG'CTG Cuts at: 2474 2707 2474 Size: 233 5696 SacII CC_GC'GG Cuts at: 2505 2505 Size: 5929 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SapI GCTCTTCn'nnn_ Cuts at: 2672 2672 Size: 5929 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 4177 5889 4177 Size: 1712 4217 SexAI A'CCwGG_T Cuts at: 2253 2253 Size: 5929 SfcI C'TryA_G Cuts at: 826 1045 3277 4412 5093 5284 826 Size: 219 2232 1135 681 191 1471 Fragments arranged by size: 2232 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2067 2323 2763 2067 Size: 256 440 5233 Fragments arranged by size: 5233 440 256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 2281 2758 1700 Size: 581 477 4871 Fragments arranged by size: 4871 581 477 SmaI CCC'GGG Cuts at: 1872 2976 3256 1872 Size: 1104 280 4545 Fragments arranged by size: 4545 1104 280 SmlI C'TyrA_G Cuts at: 820 1017 1943 4033 4934 5196 820 Size: 197 926 2090 901 262 1553 Fragments arranged by size: 2090 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3720 493 Size: 3227 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5929 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SspI AAT'ATT Cuts at: 5 52 3853 5 Size: 47 3801 2081 Fragments arranged by size: 3801 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5929 StyI C'CwwG_G Cuts at: 513 1065 2004 2042 2292 2883 3368 513 Size: 552 939 38 250 591 485 3074 Fragments arranged by size: 3074 939 591 552 485 250 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3956 3973 4126 4311 4756 325 Size: 1255 2376 17 153 185 445 1498 Fragments arranged by size: 2376 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4175 5887 96 Size: 274 80 33 51 1443 2198 1712 138 Fragments arranged by size: 2198 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2256 2288 2345 2377 5889 5921 2256 Size: 32 57 32 3512 32 2264 Fragments arranged by size: 3512 2264 57 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5929 XbaI T'CTAG_A Cuts at: 3265 3265 Size: 5929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2103 2103 Size: 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 2974 3254 1870 Size: 1104 280 4545 Fragments arranged by size: 4545 1104 280 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SfiI SrfI SwaI