(Linear) (Six-base) MAPSORT of: KIEE9571.seq Check: 9222 from: 1 to: 1224 KIEE9571.orf With 182 enzymes: SgfI * December 27, 2011 16:42 .. AccI GT'mk_AC Cuts at: 0 668 1224 Size: 668 556 AfeI AGC'GCT Cuts at: 0 454 562 1224 Size: 454 108 662 Fragments arranged by size: 662 454 108 AflIII A'CryG_T Cuts at: 0 218 1224 Size: 218 1006 ApaI G_GGCC'C Cuts at: 0 126 870 958 976 1224 Size: 126 744 88 18 248 Fragments arranged by size: 744 248 126 88 18 ApaLI G'TGCA_C Cuts at: 0 873 1224 Size: 873 351 AvaI C'yCGr_G Cuts at: 0 739 969 1143 1224 Size: 739 230 174 81 Fragments arranged by size: 739 230 174 81 BanI G'GyrC_C Cuts at: 0 60 270 316 756 813 895 1224 Size: 60 210 46 440 57 82 329 Fragments arranged by size: 440 329 210 82 60 57 46 BanII G_rGCy'C Cuts at: 0 126 870 958 976 1112 1224 Size: 126 744 88 18 136 112 Fragments arranged by size: 744 136 126 112 88 18 BbeI G_GCGC'C Cuts at: 0 64 320 760 1224 Size: 64 256 440 464 Fragments arranged by size: 464 440 256 64 BbsI GAAGACnn'nnnn_ Cuts at: 0 1066 1224 Size: 1066 158 BglI GCCn_nnn'nGGC Cuts at: 0 130 289 1088 1129 1224 Size: 130 159 799 41 95 Fragments arranged by size: 799 159 130 95 41 Bme1580I G_kGCm'C Cuts at: 0 126 818 870 877 898 958 976 1224 Size: 126 692 52 7 21 60 18 248 Fragments arranged by size: 692 248 126 60 52 21 18 7 BmgBI CAC'GTC Cuts at: 0 351 794 1224 Size: 351 443 430 Fragments arranged by size: 443 430 351 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 42 1224 Size: 42 1182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 1224 Size: 187 1037 BsaI GGTCTCn'nnnn_ Cuts at: 0 889 1224 Size: 889 335 BsaAI yAC'GTr Cuts at: 0 612 1224 Size: 612 612 BsaHI Gr'CG_yC Cuts at: 0 61 317 548 757 1224 Size: 61 256 231 209 467 Fragments arranged by size: 467 256 231 209 61 BsaWI w'CCGG_w Cuts at: 0 1104 1224 Size: 1104 120 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 783 813 1224 Size: 783 30 411 Fragments arranged by size: 783 411 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 615 757 1224 Size: 615 142 467 Fragments arranged by size: 615 467 142 BseYI C'CCAG_C Cuts at: 0 723 1170 1224 Size: 723 447 54 Fragments arranged by size: 723 447 54 BsiEI CG_ry'CG Cuts at: 0 497 828 1092 1224 Size: 497 331 264 132 Fragments arranged by size: 497 331 264 132 BsiHKAI G_wGCw'C Cuts at: 0 877 1224 Size: 877 347 BsmBI CGTCTCn'nnnn_ Cuts at: 0 636 1224 Size: 636 588 Bsp1286I G_dGCh'C Cuts at: 0 126 818 870 877 898 958 976 1112 Size: 126 692 52 7 21 60 18 136 Cuts at: 1112 1224 Size: 112 Fragments arranged by size: 692 136 126 112 60 52 21 18 7 BspEI T'CCGG_A Cuts at: 0 1104 1224 Size: 1104 120 BspHI T'CATG_A Cuts at: 0 518 1224 Size: 518 706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 173 1224 Size: 173 1051 BsrBI CCG'CTC Cuts at: 0 1029 1224 Size: 1029 195 BsrFI r'CCGG_y Cuts at: 0 276 753 827 1224 Size: 276 477 74 397 Fragments arranged by size: 477 397 276 74 BssHII G'CGCG_C Cuts at: 0 838 942 1224 Size: 838 104 282 Fragments arranged by size: 838 282 104 BssSI C'ACGA_G Cuts at: 0 16 652 1224 Size: 16 636 572 Fragments arranged by size: 636 572 16 BstEII G'GTnAC_C Cuts at: 0 444 1224 Size: 444 780 BtgI C'CryG_G Cuts at: 0 7 37 100 152 395 497 1224 Size: 7 30 63 52 243 102 727 Fragments arranged by size: 727 243 102 63 52 30 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 168 1224 Size: 168 1056 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 63 98 1224 Size: 63 35 1126 Fragments arranged by size: 1126 63 35 EaeI y'GGCC_r Cuts at: 0 40 146 179 236 281 300 307 392 Size: 40 106 33 57 45 19 7 85 Cuts at: 392 494 825 929 1089 1224 Size: 102 331 104 160 135 Fragments arranged by size: 331 160 135 106 104 102 85 57 45 40 33 19 7 EagI C'GGCC_G Cuts at: 0 494 825 1089 1224 Size: 494 331 264 135 Fragments arranged by size: 494 331 264 135 EarI CTCTTCn'nnn_ Cuts at: 0 653 667 1141 1224 Size: 653 14 474 83 Fragments arranged by size: 653 474 83 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 725 740 791 1057 1224 Size: 725 15 51 266 167 Fragments arranged by size: 725 266 167 51 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 1224 Size: 187 1037 EcoO109I rG'GnC_Cy Cuts at: 0 122 123 867 954 955 1224 Size: 122 1 744 87 1 269 Fragments arranged by size: 744 269 122 87 1 1 HaeII r_GCGC'y Cuts at: 0 64 136 295 320 456 564 760 1064 Size: 64 72 159 25 136 108 196 304 Cuts at: 1064 1224 Size: 160 Fragments arranged by size: 304 196 160 159 136 108 72 64 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 783 815 1224 Size: 783 32 409 Fragments arranged by size: 783 409 32 KasI G'GCGC_C Cuts at: 0 60 316 756 1224 Size: 60 256 440 468 Fragments arranged by size: 468 440 256 60 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 596 623 1224 Size: 596 27 601 Fragments arranged by size: 601 596 27 MscI TGG'CCA Cuts at: 0 148 181 302 931 1224 Size: 148 33 121 629 293 Fragments arranged by size: 629 293 148 121 33 MspA1I CmG'CkG Cuts at: 0 426 469 499 702 1085 1224 Size: 426 43 30 203 383 139 Fragments arranged by size: 426 383 203 139 43 30 NaeI GCC'GGC Cuts at: 0 278 755 829 1224 Size: 278 477 74 395 Fragments arranged by size: 477 395 278 74 NarI GG'CG_CC Cuts at: 0 61 317 757 1224 Size: 61 256 440 467 Fragments arranged by size: 467 440 256 61 NcoI C'CATG_G Cuts at: 0 37 1224 Size: 37 1187 NgoMIV G'CCGG_C Cuts at: 0 276 753 827 1224 Size: 276 477 74 397 Fragments arranged by size: 477 397 276 74 NspI r_CATG'y Cuts at: 0 606 1224 Size: 606 618 PasI CC'CwG_GG Cuts at: 0 948 1224 Size: 948 276 PflMI CCAn_nnn'nTGG Cuts at: 0 188 1224 Size: 188 1036 PfoI T'CCnGG_A Cuts at: 0 854 1224 Size: 854 370 PspOMI G'GGCC_C Cuts at: 0 122 866 954 972 1224 Size: 122 744 88 18 252 Fragments arranged by size: 744 252 122 88 18 PvuII CAG'CTG Cuts at: 0 469 702 1224 Size: 469 233 522 Fragments arranged by size: 522 469 233 SacII CC_GC'GG Cuts at: 0 500 1224 Size: 500 724 SapI GCTCTTCn'nnn_ Cuts at: 0 667 1224 Size: 667 557 SexAI A'CCwGG_T Cuts at: 0 248 1224 Size: 248 976 SfoI GGC'GCC Cuts at: 0 62 318 758 1224 Size: 62 256 440 466 Fragments arranged by size: 466 440 256 62 SgrAI Cr'CCGG_yG Cuts at: 0 276 753 1224 Size: 276 477 471 Fragments arranged by size: 477 471 276 SmaI CCC'GGG Cuts at: 0 971 1224 Size: 971 253 StyI C'CwwG_G Cuts at: 0 37 287 878 1224 Size: 37 250 591 346 Fragments arranged by size: 591 346 250 37 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 251 283 340 372 1224 Size: 251 32 57 32 852 Fragments arranged by size: 852 251 57 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 98 1224 Size: 98 1126 XmaI C'CCGG_G Cuts at: 0 969 1224 Size: 969 255 Enzymes that do cut: AccI AfeI AflIII ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstEII BtgI BtgZI CspCI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PspOMI PvuII SacII SapI SexAI SfoI SgrAI SmaI StyI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaBI BsgI BsiWI BsmI BsrDI BsrGI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmnI ZraI