(Circular) (Six-base) MAPSORT of: pFN21AE2452.seq Check: 933 from: 1 to: 6292 pFN21AE2452 6292 bp With 182 enzymes: SgfI * February 5, 2013 11:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2066 3336 3450 2066 Size: 1270 114 4908 Fragments arranged by size: 4908 1270 114 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2565 2769 4102 278 Size: 53 83 186 936 1029 204 1333 2468 Fragments arranged by size: 2468 1333 1029 936 204 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 1937 Size: 1698 4594 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 2690 3209 4355 5739 6007 1632 Size: 597 461 519 1146 1384 268 1917 Fragments arranged by size: 1917 1384 1146 597 519 461 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2927 3447 1184 Size: 1743 520 4029 Fragments arranged by size: 4029 1743 520 AlwNI CAG_nnn'CTG Cuts at: 4035 5607 4035 Size: 1572 4720 ApaI G_GGCC'C Cuts at: 2012 3112 2012 Size: 1100 5192 ApaLI G'TGCA_C Cuts at: 2516 3059 3422 4349 5505 2516 Size: 543 363 927 1156 3303 Fragments arranged by size: 3303 1156 927 543 363 ApoI r'AATT_y Cuts at: 1493 3601 3827 3979 6088 1493 Size: 2108 226 152 2109 1697 Fragments arranged by size: 2109 2108 1697 226 152 AscI GG'CGCG_CC Cuts at: 2225 2225 Size: 6292 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3617 1870 Size: 73 1674 4545 Fragments arranged by size: 4545 1674 73 BamHI G'GATC_C Cuts at: 2427 3622 3989 2427 Size: 1195 367 4730 Fragments arranged by size: 4730 1195 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 2524 2717 3305 3613 Size: 325 217 627 446 291 193 588 308 Cuts at: 3613 5068 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 588 446 325 308 291 217 193 BanII G_rGCy'C Cuts at: 1391 2012 2113 2549 3112 3171 3611 1391 Size: 621 101 436 563 59 440 4072 Fragments arranged by size: 4072 621 563 440 436 101 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2854 2923 3037 928 Size: 971 955 69 114 4183 Fragments arranged by size: 4183 971 955 114 69 BbvCI CC'TCA_GC Cuts at: 3357 3357 Size: 6292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3133 4903 136 Size: 107 122 71 631 605 1461 1770 1525 Fragments arranged by size: 1770 1525 1461 631 605 122 107 71 BglII A'GATC_T Cuts at: 2754 6287 2754 Size: 3533 2759 BlpI GC'TnA_GC Cuts at: 2045 2091 2283 3194 3709 2045 Size: 46 192 911 515 4628 Fragments arranged by size: 4628 911 515 192 46 Bme1580I G_kGCm'C Cuts at: 1790 2012 2238 2513 2520 2529 2720 3063 3112 Size: 222 226 275 7 9 191 343 49 Cuts at: 3112 3129 3308 3426 4353 5509 1790 Size: 17 179 118 927 1156 2573 Fragments arranged by size: 2573 1156 927 343 275 226 222 191 179 118 49 17 9 7 BmgBI CAC'GTC Cuts at: 1318 2251 1318 Size: 933 5359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2130 2530 2689 Size: 416 53 83 399 24 713 400 159 Cuts at: 2689 3106 4023 4981 442 Size: 417 917 958 1753 Fragments arranged by size: 1753 958 917 713 417 416 400 399 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2141 2141 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 2663 3236 4952 1239 Size: 897 527 573 1716 2579 Fragments arranged by size: 2579 1716 897 573 527 Bpu10I CC'TnA_GC Cuts at: 3357 3357 Size: 6292 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4417 5282 5580 4417 Size: 865 298 5129 Fragments arranged by size: 5129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 4955 882 Size: 617 613 2843 2219 Fragments arranged by size: 2843 2219 617 613 BsaAI yAC'GTr Cuts at: 493 1204 4083 493 Size: 711 2879 2702 Fragments arranged by size: 2879 2702 711 BsaBI GATnn'nnATC Cuts at: 2426 6286 2426 Size: 3860 2432 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2562 2766 4099 4481 Size: 53 83 186 936 1029 204 1333 382 Cuts at: 4481 5116 275 Size: 635 1451 Fragments arranged by size: 1451 1333 1029 936 635 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2486 2516 2531 Size: 30 165 30 708 30 345 30 15 Cuts at: 2531 2561 2858 2888 3053 3083 1178 Size: 30 297 30 165 30 4387 Fragments arranged by size: 4387 708 345 297 165 165 30 30 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2119 3251 3272 3275 1186 Size: 933 1132 21 3 4203 Fragments arranged by size: 4203 1132 933 21 3 BseYI C'CCAG_C Cuts at: 2071 2220 3074 5495 5991 2071 Size: 149 854 2421 496 2372 Fragments arranged by size: 2421 2372 854 496 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3300 3314 1163 Size: 573 1564 14 4141 Fragments arranged by size: 4141 1564 573 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 2549 3054 3063 3171 3426 4353 4438 5509 Size: 29 505 9 108 255 927 85 1071 Cuts at: 5509 2520 Size: 3303 Fragments arranged by size: 3303 1071 927 505 255 108 85 29 9 BsmI GAATG_Cn' Cuts at: 3803 3896 3803 Size: 93 6199 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2113 2238 2513 2520 2529 2549 Size: 399 222 101 125 275 7 9 20 Cuts at: 2549 2720 3054 3063 3112 3129 3171 3308 3426 Size: 171 334 9 49 17 42 137 118 Cuts at: 3426 3611 4353 4438 5509 1391 Size: 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 334 275 222 185 171 137 125 118 101 85 49 42 20 17 9 9 7 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 4181 5911 4181 Size: 1730 4562 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2066 2877 3006 3272 3336 3450 3647 844 Size: 1222 811 129 266 64 114 197 3489 Fragments arranged by size: 3489 1222 811 266 197 129 114 64 BsrBI CCG'CTC Cuts at: 1394 1823 2688 2800 2983 3069 4179 1394 Size: 429 865 112 183 86 1110 3507 Fragments arranged by size: 3507 1110 865 429 183 112 86 BsrDI GCAATG_nn' Cuts at: 65 1248 2147 3429 4787 4961 65 Size: 1183 899 1282 1358 174 1396 Fragments arranged by size: 1396 1358 1282 1183 899 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2184 3147 4936 5123 1700 Size: 110 374 963 1789 187 2869 Fragments arranged by size: 2869 1789 963 374 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 2225 1925 Size: 300 5992 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 2524 3312 794 Size: 1730 788 3774 Fragments arranged by size: 3774 1730 788 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstXI CCAn_nnnn'nTGG Cuts at: 2971 2971 Size: 6292 BstYI r'GATC_y Cuts at: 1972 2427 2754 3510 3622 3989 4374 4391 5843 Size: 455 327 756 112 367 385 17 1452 Cuts at: 5843 5929 5941 6287 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 756 455 385 367 346 327 112 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3343 3343 Size: 6292 BtgI C'CryG_G Cuts at: 513 1065 2004 2142 2505 2712 3162 3319 3495 Size: 552 939 138 363 207 450 157 176 Cuts at: 3495 513 Size: 3310 Fragments arranged by size: 3310 939 552 450 363 207 176 157 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2263 487 Size: 683 56 1037 4516 Fragments arranged by size: 4516 1037 683 56 BtsI GCAGTG_nn' Cuts at: 2478 3808 4602 4622 2478 Size: 1330 794 20 4148 Fragments arranged by size: 4148 1330 794 20 ClaI AT'CG_AT Cuts at: 1554 2292 1554 Size: 738 5554 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2365 2400 529 Size: 35 1801 35 4421 Fragments arranged by size: 4421 1801 35 35 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DraIII CAC_nnn'GTG Cuts at: 2524 2901 3100 2524 Size: 377 199 5716 Fragments arranged by size: 5716 377 199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 3322 3739 Size: 54 1425 50 275 18 702 790 417 Cuts at: 3739 4628 6257 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 790 702 417 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 2207 2504 2807 3469 4222 1353 Size: 842 12 297 303 662 753 3423 Fragments arranged by size: 3423 842 753 662 303 297 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2547 3169 2547 Size: 622 5670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2229 2663 2690 3209 3236 4355 Size: 393 504 93 434 27 519 27 1119 Cuts at: 4355 4952 5739 6007 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1119 787 597 519 504 434 393 268 93 27 27 EcoO109I rG'GnC_Cy Cuts at: 2009 2407 3108 3109 3339 3347 2009 Size: 398 701 1 230 8 4954 Fragments arranged by size: 4954 701 398 230 8 1 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 2098 5439 1678 Size: 420 3341 2531 Fragments arranged by size: 3341 2531 420 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2262 2294 3446 Size: 32 135 32 539 32 119 32 1152 Cuts at: 3446 3478 4933 4965 5007 5039 1373 Size: 32 1455 32 42 32 2626 Fragments arranged by size: 2626 1455 1152 539 135 119 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2901 5406 5590 1305 Size: 1596 2505 184 2007 Fragments arranged by size: 2505 2007 1596 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4470 Fragments arranged by size: 4470 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2909 4250 4609 4768 518 Size: 648 1128 615 1341 359 159 2042 Fragments arranged by size: 2042 1341 1128 648 615 359 159 MspA1I CmG'CkG Cuts at: 1606 2220 3708 4385 5533 5778 1606 Size: 614 1488 677 1148 245 2120 Fragments arranged by size: 2120 1488 1148 677 614 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3495 513 Size: 552 939 1491 3310 Fragments arranged by size: 3310 1491 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3279 3403 3650 5195 1132 Size: 97 2050 124 247 1545 2229 Fragments arranged by size: 2229 2050 1545 247 124 97 PasI CC'CwG_GG Cuts at: 1348 2106 1348 Size: 758 5534 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 2078 2331 1108 Size: 970 253 5069 Fragments arranged by size: 5069 970 253 PfoI T'CCnGG_A Cuts at: 2113 2612 2113 Size: 499 5793 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2407 3347 2407 Size: 940 5352 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PspOMI G'GGCC_C Cuts at: 2008 3108 2008 Size: 1100 5192 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2987 3019 2987 Size: 32 6260 PstI C_TGCA'G Cuts at: 830 2555 3317 3372 3644 830 Size: 1725 762 55 272 3478 Fragments arranged by size: 3478 1725 762 272 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 SacI G_AGCT'C Cuts at: 2549 3171 2549 Size: 622 5670 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SanDI GG'GwC_CC Cuts at: 2407 2407 Size: 6292 SapI GCTCTTCn'nnn_ Cuts at: 2195 2807 2195 Size: 612 5680 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SfcI C'TryA_G Cuts at: 826 1045 2551 3313 3368 3640 4775 5456 5647 Size: 219 1506 762 55 272 1135 681 191 Cuts at: 5647 826 Size: 1471 Fragments arranged by size: 1506 1471 1135 762 681 272 219 191 55 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4396 5297 5559 820 Size: 197 926 2453 901 262 1553 Fragments arranged by size: 2453 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 3351 3495 3731 513 Size: 552 939 483 864 144 236 3074 Fragments arranged by size: 3074 939 864 552 483 236 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2512 3088 3095 4319 4336 4489 4674 Size: 1255 932 576 7 1224 17 153 185 Cuts at: 4674 5119 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1224 932 576 445 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4538 6250 96 Size: 274 80 33 51 1443 2561 1712 138 Fragments arranged by size: 2561 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2486 2518 3053 3085 6252 6284 2486 Size: 32 535 32 3167 32 2494 Fragments arranged by size: 3167 2494 535 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 3332 2638 Size: 694 5598 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 4421 4421 Size: 6292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2563 2767 4100 276 Size: 53 83 186 936 1029 204 1333 2468 Fragments arranged by size: 2468 1333 1029 936 204 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI