(Circular) (Six-base) MAPSORT of: pFN21AE1993.seq Check: 826 from: 1 to: 6421 pFN21AE1993 6421 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2220 2258 3569 4231 Size: 53 83 186 936 684 38 1311 662 Cuts at: 4231 278 Size: 2468 Fragments arranged by size: 2468 1311 936 684 662 186 83 53 38 AccI GT'mk_AC Cuts at: 1937 3764 1937 Size: 1827 4594 Acc65I G'GTAC_C Cuts at: 3742 3742 Size: 6421 AclI AA'CG_TT Cuts at: 1563 4548 4921 1563 Size: 2985 373 3063 Fragments arranged by size: 3063 2985 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4484 5868 6136 1632 Size: 2852 1384 268 1917 Fragments arranged by size: 2852 1917 1384 268 AfeI AGC'GCT Cuts at: 2466 2466 Size: 6421 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6224 AflIII A'CryG_T Cuts at: 1203 2671 5320 6297 1203 Size: 1468 2649 977 1327 Fragments arranged by size: 2649 1468 1327 977 AgeI A'CCGG_T Cuts at: 5252 5252 Size: 6421 AhdI GACnn_n'nnGTC Cuts at: 2392 5150 2392 Size: 2758 3663 AleI CACnn'nnGTG Cuts at: 1166 2765 1166 Size: 1599 4822 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6421 AlwNI CAG_nnn'CTG Cuts at: 4164 5736 4164 Size: 1572 4849 ApaI G_GGCC'C Cuts at: 2663 2663 Size: 6421 ApaLI G'TGCA_C Cuts at: 4478 5634 4478 Size: 1156 5265 ApoI r'AATT_y Cuts at: 1493 2488 3730 3956 4108 6217 1493 Size: 995 1242 226 152 2109 1697 Fragments arranged by size: 2109 1697 1242 995 226 152 AseI AT'TA_AT Cuts at: 160 1032 4975 160 Size: 872 3943 1606 Fragments arranged by size: 3943 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 AvaI C'yCGr_G Cuts at: 1870 1943 2663 3290 3746 1870 Size: 73 720 627 456 4545 Fragments arranged by size: 4545 720 627 456 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3430 3463 3430 Size: 33 6388 BamHI G'GATC_C Cuts at: 2637 3215 3751 4118 2637 Size: 578 536 367 4940 Fragments arranged by size: 4940 578 536 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 3065 3374 3682 3742 Size: 325 217 627 792 486 309 308 60 Cuts at: 3742 5197 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 792 627 486 325 309 308 217 60 BanII G_rGCy'C Cuts at: 1391 2663 2838 2930 3740 1391 Size: 1272 175 92 810 4072 Fragments arranged by size: 4072 1272 810 175 92 BbeI G_GCGC'C Cuts at: 2583 3686 2583 Size: 1103 5318 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2045 2177 3169 928 Size: 971 146 132 992 4180 Fragments arranged by size: 4180 992 971 146 132 BbvCI CC'TCA_GC Cuts at: 2787 2787 Size: 6421 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2600 2634 4612 4646 1903 Size: 34 663 34 1978 34 3678 Fragments arranged by size: 3678 1978 663 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4313 5523 4313 Size: 1210 5211 BclI T'GATC_A Cuts at: 1548 2058 2127 2242 2313 1548 Size: 510 69 115 71 5656 Fragments arranged by size: 5656 510 115 71 69 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3026 3557 5032 Size: 107 122 71 631 605 1354 531 1475 Cuts at: 5032 136 Size: 1525 Fragments arranged by size: 1525 1475 1354 631 605 531 122 107 71 BglII A'GATC_T Cuts at: 6416 6416 Size: 6421 BlpI GC'TnA_GC Cuts at: 2678 3471 3838 2678 Size: 793 367 5261 Fragments arranged by size: 5261 793 367 Bme1580I G_kGCm'C Cuts at: 1790 2663 3001 3068 4482 5638 1790 Size: 873 338 67 1414 1156 2573 Fragments arranged by size: 2573 1414 1156 873 338 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6421 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2896 3129 4152 Size: 416 53 83 399 24 1479 233 1023 Cuts at: 4152 5110 442 Size: 958 1753 Fragments arranged by size: 1753 1479 1023 958 416 399 233 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6421 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2203 2203 Size: 6421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2896 2928 2966 3386 3531 5081 1239 Size: 1657 32 38 420 145 1550 2579 Fragments arranged by size: 2579 1657 1550 420 145 38 32 Bpu10I CC'TnA_GC Cuts at: 2334 2787 2334 Size: 453 5968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2944 4546 5411 5709 2944 Size: 1602 865 298 3656 Fragments arranged by size: 3656 1602 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2970 5084 882 Size: 617 1471 2114 2219 Fragments arranged by size: 2219 2114 1471 617 BsaAI yAC'GTr Cuts at: 493 1204 4212 493 Size: 711 3008 2702 Fragments arranged by size: 3008 2702 711 BsaBI GATnn'nnATC Cuts at: 2198 6415 2198 Size: 4217 2204 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 2255 2580 3530 Size: 53 83 186 936 684 38 325 950 Cuts at: 3530 3566 3683 4228 4610 5245 275 Size: 36 117 545 382 635 1451 Fragments arranged by size: 1451 950 936 684 635 545 382 325 186 117 83 53 38 36 BsaWI w'CCGG_w Cuts at: 3058 3597 4854 5252 5526 5673 6084 3058 Size: 539 1257 398 274 147 411 3395 Fragments arranged by size: 3395 1257 539 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3074 3104 3529 3559 1178 Size: 30 165 30 1671 30 425 30 4040 Fragments arranged by size: 4040 1671 425 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2178 2468 2639 2705 3095 3544 1186 Size: 992 290 171 66 390 449 4063 Fragments arranged by size: 4063 992 449 390 290 171 66 BseYI C'CCAG_C Cuts at: 2297 3669 5624 6120 2297 Size: 1372 1955 496 2598 Fragments arranged by size: 2598 1955 1372 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3590 1163 Size: 573 1854 3994 Fragments arranged by size: 3994 1854 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2050 3871 4632 5660 1540 Size: 275 186 49 1821 761 1028 2301 Fragments arranged by size: 2301 1821 1028 761 275 186 49 BsiHKAI G_wGCw'C Cuts at: 3637 4482 4567 5638 3637 Size: 845 85 1071 4420 Fragments arranged by size: 4420 1071 845 85 BsmI GAATG_Cn' Cuts at: 3932 4025 3932 Size: 93 6328 BsmBI CGTCTCn'nnnn_ Cuts at: 3573 3573 Size: 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2663 2838 2930 3001 3068 3637 3740 Size: 399 873 175 92 71 67 569 103 Cuts at: 3740 4482 4567 5638 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 873 742 569 399 175 103 92 85 71 67 BspEI T'CCGG_A Cuts at: 3058 3597 6084 3058 Size: 539 2487 3395 Fragments arranged by size: 3395 2487 539 BspHI T'CATG_A Cuts at: 4310 6040 4310 Size: 1730 4691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3612 3776 844 Size: 2768 164 3489 Fragments arranged by size: 3489 2768 164 BsrBI CCG'CTC Cuts at: 1394 1823 3085 4308 1394 Size: 429 1262 1223 3507 Fragments arranged by size: 3507 1262 1223 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2616 3001 3204 4916 5090 65 Size: 1183 1368 385 203 1712 174 1396 Fragments arranged by size: 1712 1396 1368 1183 385 203 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5065 5252 1700 Size: 110 3255 187 2869 Fragments arranged by size: 3255 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2502 96 Size: 2406 4015 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6421 BssSI C'ACGA_G Cuts at: 2041 2498 3489 4481 5493 2041 Size: 457 991 992 1012 2969 Fragments arranged by size: 2969 1012 992 991 457 BstAPI GCAn_nnn'nTGC Cuts at: 794 2397 3318 794 Size: 1603 921 3897 Fragments arranged by size: 3897 1603 921 BstBI TT'CG_AA Cuts at: 5225 5225 Size: 6421 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6421 BstYI r'GATC_y Cuts at: 1972 2637 2644 3215 3751 4118 4503 4520 5972 Size: 665 7 571 536 367 385 17 1452 Cuts at: 5972 6058 6070 6416 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 665 571 536 385 367 346 86 17 12 7 Bsu36I CC'TnA_GG Cuts at: 2688 2688 Size: 6421 BtgI C'CryG_G Cuts at: 513 1065 3322 513 Size: 552 2257 3612 Fragments arranged by size: 3612 2257 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2713 2779 487 Size: 683 56 1487 66 4129 Fragments arranged by size: 4129 1487 683 66 56 BtsI GCAGTG_nn' Cuts at: 2841 3001 3015 3937 4731 4751 2841 Size: 160 14 922 794 20 4511 Fragments arranged by size: 4511 922 794 160 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6386 DraI TTT'AAA Cuts at: 3725 3725 Size: 6421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2984 5428 809 Size: 507 1668 2444 1802 Fragments arranged by size: 2444 1802 1668 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3558 3694 3868 Size: 54 1425 50 275 18 1728 136 174 Cuts at: 3868 4757 6386 8 Size: 889 1629 43 Fragments arranged by size: 1728 1629 1425 889 275 174 136 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3868 1537 Size: 275 2056 4090 Fragments arranged by size: 4090 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4351 1353 Size: 2998 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5008 5382 5528 387 Size: 1064 138 3419 374 146 1280 Fragments arranged by size: 3419 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2896 2928 2966 3386 3531 4484 5081 Size: 393 1264 32 38 420 145 953 597 Cuts at: 5081 5868 6136 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1264 953 787 597 420 393 268 145 38 32 EcoNI CCTnn'n_nnAGG Cuts at: 3195 3195 Size: 6421 EcoO109I rG'GnC_Cy Cuts at: 2660 3241 3586 2660 Size: 581 345 5495 Fragments arranged by size: 5495 581 345 EcoRI G'AATT_C Cuts at: 3730 4108 3730 Size: 378 6043 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3061 3061 Size: 6421 FspI TGC'GCA Cuts at: 4927 4927 Size: 6421 HaeII r_GCGC'y Cuts at: 1678 2468 2583 3686 5568 1678 Size: 790 115 1103 1882 2531 Fragments arranged by size: 2531 1882 1103 790 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2035 2067 2075 2107 2227 Size: 32 135 32 463 32 8 32 120 Cuts at: 2227 2259 2378 2410 2629 2661 2917 2949 3074 Size: 32 119 32 219 32 256 32 125 Cuts at: 3074 3106 3529 3561 5062 5094 5136 5168 1373 Size: 32 423 32 1501 32 42 32 2626 Fragments arranged by size: 2626 1501 463 423 256 219 135 125 120 119 42 32 32 32 32 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 3765 4011 669 Size: 988 281 1827 246 3079 Fragments arranged by size: 3079 1827 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6421 HpaI GTT'AAC Cuts at: 4011 4011 Size: 6421 KasI G'GCGC_C Cuts at: 2579 3682 2579 Size: 1103 5318 KpnI G_GTAC'C Cuts at: 3746 3746 Size: 6421 MfeI C'AATT_G Cuts at: 4020 4020 Size: 6421 MluI A'CGCG_T Cuts at: 6297 6297 Size: 6421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2960 5535 5719 1305 Size: 1655 2575 184 2007 Fragments arranged by size: 2575 2007 1655 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4599 Fragments arranged by size: 4599 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2765 3209 4379 4738 4897 518 Size: 648 1599 444 1170 359 159 2042 Fragments arranged by size: 2042 1599 1170 648 444 359 159 MspA1I CmG'CkG Cuts at: 1606 2752 3324 3837 4514 5662 5907 1606 Size: 1146 572 513 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 677 572 513 245 NarI GG'CG_CC Cuts at: 2580 3683 2580 Size: 1103 5318 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5869 NdeI CA'TA_TG Cuts at: 387 387 Size: 6421 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6421 NotI GC'GGCC_GC Cuts at: 3868 3868 Size: 6421 NspI r_CATG'y Cuts at: 1132 1229 2420 2675 3386 3779 5324 1132 Size: 97 1191 255 711 393 1545 2229 Fragments arranged by size: 2229 1545 1191 711 393 255 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6421 PciI A'CATG_T Cuts at: 2671 5320 2671 Size: 2649 3772 PflMI CCAn_nnn'nTGG Cuts at: 1108 3145 1108 Size: 2037 4384 PfoI T'CCnGG_A Cuts at: 2189 2448 2568 2640 3363 2189 Size: 259 120 72 723 5247 Fragments arranged by size: 5247 723 259 120 72 PmeI GTTT'AAAC Cuts at: 3725 3725 Size: 6421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4490 4522 6003 6035 4490 Size: 32 1481 32 4876 Fragments arranged by size: 4876 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3241 3241 Size: 6421 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6421 PsiI TTA'TAA Cuts at: 3991 3991 Size: 6421 PspOMI G'GGCC_C Cuts at: 2659 2659 Size: 6421 PstI C_TGCA'G Cuts at: 830 3773 830 Size: 2943 3478 PvuI CG_AT'CG Cuts at: 2001 2050 2001 Size: 49 6372 SacII CC_GC'GG Cuts at: 3325 3325 Size: 6421 SalI G'TCGA_C Cuts at: 1936 3763 1936 Size: 1827 4594 SanDI GG'GwC_CC Cuts at: 3241 3241 Size: 6421 SbfI CC_TGCA'GG Cuts at: 3773 3773 Size: 6421 ScaI AGT'ACT Cuts at: 2556 4669 6381 2556 Size: 2113 1712 2596 Fragments arranged by size: 2596 2113 1712 SexAI A'CCwGG_T Cuts at: 3228 3228 Size: 6421 SfcI C'TryA_G Cuts at: 826 1045 3030 3769 4904 5585 5776 826 Size: 219 1985 739 1135 681 191 1471 Fragments arranged by size: 1985 1471 1135 739 681 219 191 SfoI GGC'GCC Cuts at: 2581 3684 2581 Size: 1103 5318 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6421 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6421 SmaI CCC'GGG Cuts at: 1872 3748 1872 Size: 1876 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2923 3290 4525 5426 5688 820 Size: 197 926 980 367 1235 901 262 1553 Fragments arranged by size: 1553 1235 980 926 901 367 262 197 SnaBI TAC'GTA Cuts at: 493 4212 493 Size: 3719 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6421 SphI G_CATG'C Cuts at: 1132 3779 1132 Size: 2647 3774 SspI AAT'ATT Cuts at: 5 52 2029 4345 5 Size: 47 1977 2316 2081 Fragments arranged by size: 2316 2081 1977 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6421 StyI C'CwwG_G Cuts at: 513 1065 2275 2283 2763 2892 3860 513 Size: 552 1210 8 480 129 968 3074 Fragments arranged by size: 3074 1210 968 552 480 129 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 4448 4465 4618 4803 5248 325 Size: 1255 2103 765 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 765 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 2554 3294 Size: 274 80 33 51 1443 525 52 740 Cuts at: 3294 4667 6379 96 Size: 1373 1712 138 Fragments arranged by size: 1712 1443 1373 740 525 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 3040 3072 6381 6413 2808 Size: 32 200 32 3309 32 2816 Fragments arranged by size: 3309 2816 200 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3230 1316 Size: 1914 4507 XbaI T'CTAG_A Cuts at: 3757 3757 Size: 6421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2603 2290 Size: 313 6108 XhoI C'TCGA_G Cuts at: 1943 3290 1943 Size: 1347 5074 XmaI C'CCGG_G Cuts at: 1870 3746 1870 Size: 1876 4545 XmnI GAAnn'nnTTC Cuts at: 3422 4550 3422 Size: 1128 5293 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2218 2256 3567 4229 Size: 53 83 186 936 684 38 1311 662 Cuts at: 4229 276 Size: 2468 Fragments arranged by size: 2468 1311 936 684 662 186 83 53 38 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SfiI SrfI SwaI