Restriction map for
pFN21AE3985
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRV EcoT14I EcoT22I FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AlwNI 1037 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 238 CCTGAGG CC^T[ACGT]AGG
AseI 487 ATTAAT AT^TAAT
AsnI 487 ATTAAT AT^TAAT
AspHI 312 GAGCAC G[AT]GC[AT]^C
BamHI 888 GGATCC G^GATCC
BbvI 29 GCAGC GCAGC(8/12)
BcgI 95 GCAGTAAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 164 TGATCA T^GATCA
BmyI 312 GAGCAC G[AGT]GC[ACT]^C
BpmI 823 CTCCAG CTGGAG(16/14)
BsaWI 579 ACCGGA [AT]^CCGG[AT]
BsiHKAI 312 GAGCAC G[AT]GC[AT]^C
Bsp1286I 312 GAGCAC G[AGT]GC[ACT]^C
BstBI 433 TTCGAA TT^CGAA
BstEII 9 GGTGACC G^GT[ACGT]ACC
BstPI 9 GGTGACC G^GT[ACGT]ACC
BstYI 888 GGATCC [GA]^GATC[CT]
Bsu36I 238 CCTGAGG CC^T[ACGT]AGG
DraIII 1154 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 364 GACAAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1126 GAAGAG CTCTTC(1/4)
Eco81I 238 CCTGAGG CC^T[ACGT]AGG
EcoO65I 9 GGTGACC G^GT[ACGT]ACC
EcoRI 567 GAATTC G^AATTC
FauI 6 GCGGG CCCGC(4/6)
FbaI 164 TGATCA T^GATCA
HapII 580 CCGG C^CGG
HgaI 456 GCGTC GACGC(5/10)
HpaII 580 CCGG C^CGG
Ksp632I 1126 GAAGAG CTCTTC(1/4)
MaeII 1106 ACGT A^CGT
MflI 888 GGATCC [GA]^GATC[CT]
MspA1I 738 CCGCTG C[AC]G^C[GT]G
MspI 580 CCGG C^CGG
NheI 627 GCTAGC G^CTAGC
NspV 433 TTCGAA TT^CGAA
PshAI 415 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 487 ATTAAT AT^TAAT
ScaI 428 AGTACT AGT^ACT
SfuI 433 TTCGAA TT^CGAA
TseI 29 GCAGC G^C[AT]GC
XhoII 888 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 567 GAATTC [GA]^AATT[CT]
610 GAATTT
AfaI 294 GTAC GT^AC
429 GTAC
AflIII 353 ACATGT A^C[GA][CT]GT
1148 ACATGT
ApoI 567 GAATTC [GA]^AATT[CT]
610 GAATTT
AvaII 699 GGACC G^G[AT]CC
1100 GGTCC
BalI 89 TGGCCA TGG^CCA
725 TGGCCA
BfaI 550 CTAG C^TAG
628 CTAG
BsaJI 660 CCAGGG C^C[ACGT][ACGT]GG
825 CCAGGG
BseRI 398 GAGGAG GAGGAG(10/8)
1022 GAGGAG
BspLU11I 353 ACATGT A^CATGT
1148 ACATGT
BsrI 266 ACTGG ACTGG(1/-1)
119 CCAGT
BssKI 660 CCAGG ^CC[ACGT]GG
825 CCAGG
BstF5I 863 CATCC GGATG(2/0)
171 GGATG
BstNI 660 CCAGG CC^[AT]GG
825 CCAGG
EaeI 89 TGGCCA [CT]^GGCC[GA]
725 TGGCCA
EcoRII 660 CCAGG ^CC[AT]GG
825 CCAGG
Fnu4HI 29 GCAGC GC^[ACGT]GC
737 GCCGC
FokI 863 CATCC GGATG(9/13)
171 GGATG
HphI 9 GGTGA GGTGA(8/7)
592 TCACC
ItaI 29 GCAGC GC^[ACGT]GC
737 GCCGC
MaeI 550 CTAG C^TAG
628 CTAG
MluNI 89 TGGCCA TGG^CCA
725 TGGCCA
MscI 89 TGGCCA TGG^CCA
725 TGGCCA
MslI 117 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
315 CACTTTAGTG
MvaI 660 CCAGG CC^[AT]GG
825 CCAGG
MwoI 853 GCAGAAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1027 GCAACTCTGGC
NlaIV 888 GGATCC GG[ACGT]^[ACGT]CC
1099 GGGTCC
PleI 532 GACTC GAGTC(4/5)
830 GAGTC
RsaI 294 GTAC GT^AC
429 GTAC
ScrFI 660 CCAGG CC^[ACGT]GG
825 CCAGG
TaqI 434 TCGA T^CGA
705 TCGA
Tsp45I 10 GTGAC ^GT[GC]AC
1152 GTCAC
TthHB8I 434 TCGA T^CGA
705 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI AlwI BsiYI BslI
Cac8I Cfr13I DdeI DpnII HaeIII
HinfI MaeIII MboI MboII MnlI
MseI NdeII NlaIII NspI Sau3AI
Sau96I SfaNI TfiI Tru9I Tsp509I
TspRI