################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstF5I BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AflIII 272 ACACGT A^C[GA][CT]GT AlwI 153 GATCC GGATC(4/5) AlwNI 43 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 576 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 121 GGCGCC G^G[CT][GA]CC BanII 594 GGGCTC G[GA]GC[CT]^C BbeI 121 GGCGCC GGCGC^C BbrPI 193 CACGTG CAC^GTG BcgI 341 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 138 GCTGAGC GC^T[ACGT]AGC BmyI 594 GGGCTC G[AGT]GC[ACT]^C BpmI 400 CTGGAG CTGGAG(16/14) Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC BsgI 196 GTGCAG GTGCAG(16/14) BsmFI 113 GGGAC GGGAC(10/14) Bsp1286I 594 GGGCTC G[AGT]GC[ACT]^C BspMI 198 GCAGGT ACCTGC(4/8) BssSI 436 CACGAG CACGAG(-5/-1) BstAPI 371 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 201 GGTCACC G^GT[ACGT]ACC BstPI 201 GGTCACC G^GT[ACGT]ACC BstYI 152 AGATCC [GA]^GATC[CT] CelII 138 GCTGAGC GC^T[ACGT]AGC DraIII 30 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 366 GAAGAG CTCTTC(1/4) EcoO65I 201 GGTCACC G^GT[ACGT]ACC EcoT14I 537 CCAAGG C^C[AT][AT]GG HgaI 210 GCGTC GACGC(5/10) KasI 121 GGCGCC G^GCGCC Ksp632I 366 GAAGAG CTCTTC(1/4) MaeIII 202 GTCAC ^GT[ACGT]AC MboII 366 GAAGA GAAGA(8/7) MflI 152 AGATCC [GA]^GATC[CT] MseI 98 TTAA T^TAA MslI 321 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 121 GGCGCC GG^CGCC PmaCI 193 CACGTG CAC^GTG PmlI 193 CACGTG CAC^GTG SapI 366 GAAGAGC GCTCTTC(1/4) SfcI 171 CTACAG C^T[GA][CT]AG SfoI 121 GGCGCC GGC^GCC StyI 537 CCAAGG C^C[AT][AT]GG TaqI 584 TCGA T^CGA Tru9I 98 TTAA T^TAA Tsp45I 202 GTCAC ^GT[GC]AC Tsp509I 94 AATT ^AATT TspRI 183 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 584 TCGA T^CGA XcmI 251 CCAAGCTCATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 152 AGATCC [GA]^GATC[CT] XmnI 576 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 121 GGCGCC G[GA]^CG[CT]C 209 GGCGTC BsaAI 106 TACGTG [CT]AC^GT[GA] 193 CACGTG BsaHI 121 GGCGCC G[GA]^CG[CT]C 209 GGCGTC BsmAI 265 GAGAC GTCTC(1/5) 87 GTCTC BsmBI 86 CGTCTC CGTCTC(1/5) 265 GAGACG BsrI 102 CCAGT ACTGG(1/-1) 182 CCAGT BstNI 117 CCAGG CC^[AT]GG 480 CCAGG DpnII 153 GATC ^GATC 393 GATC EcoRII 117 CCAGG ^CC[AT]GG 480 CCAGG HaeII 121 GGCGCC [GA]GCGC^[CT] 527 AGCGCC Hin1I 121 GGCGCC G[GA]^CG[CT]C 209 GGCGTC HinfI 51 GATTC G^A[ACGT]TC 78 GAATC HphI 81 TCACC GGTGA(8/7) 203 TCACC MboI 153 GATC ^GATC 393 GATC MvaI 117 CCAGG CC^[AT]GG 480 CCAGG NdeII 153 GATC ^GATC 393 GATC NlaIII 126 CATG CATG^ 450 CATG Sau3AI 153 GATC ^GATC 393 GATC SfaNI 215 GCATC GCATC(5/9) 293 GCATC TfiI 51 GATTC G^A[AT]TC 78 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BbvI BcnI BsaJI BsiYI BslI BssKI BstUI Cac8I CfoI Cfr13I DdeI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MnlI MspA1I MspI MvnI MwoI NciI NlaIV PvuII RsaI Sau96I ScrFI TseI