################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NruI NsiI NspI NspV PacI PflFI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1486 GACGTC GACGT^C AgeI 104 ACCGGT A^CCGGT AhdI 1555 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1233 GATCC GGATC(4/5) AspEI 1555 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1652 TGGCCA TGG^CCA BbrPI 1703 CACGTG CAC^GTG BcgI 351 CGAGAAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1444 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1169 AGATCT A^GATCT BspHI 1055 TCATGA T^CATGA BsrFI 104 ACCGGT [GA]^CCGG[CT] BsrI 1140 CCAGT ACTGG(1/-1) BstXI 1325 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 104 ACCGGT [GA]^CCGG[CT] DrdI 580 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1555 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 966 GAAGAG CTCTTC(1/4) HgaI 8 GCGTC GACGC(5/10) HincII 1735 GTTGAC GT[CT]^[GA]AC HindII 1735 GTTGAC GT[CT]^[GA]AC Ksp632I 966 GAAGAG CTCTTC(1/4) KspI 895 CCGCGG CCGC^GG MluNI 1652 TGGCCA TGG^CCA MscI 1652 TGGCCA TGG^CCA MslI 591 CACACCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 1225 GCTAGC G^CTAGC NotI 891 GCGGCCGC GC^GGCCGC PaeR7I 1045 CTCGAG C^TCGAG PinAI 104 ACCGGT A^CCGGT PmaCI 1703 CACGTG CAC^GTG PmlI 1703 CACGTG CAC^GTG PshAI 145 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1055 TCATGA T^CATGA SacI 1042 GAGCTC GAGCT^C SacII 895 CCGCGG CCGC^GG SapI 966 GAAGAGC GCTCTTC(1/4) SbfI 996 CCTGCAGG CCTGCA^GG SfiI 1443 GGCCGCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 221 CCCGGG CCC^GGG Sse8387I 996 CCTGCAGG CCTGCA^GG StuI 956 AGGCCT AGG^CCT TfiI 1617 GATTC G^A[AT]TC XcmI 164 CCAGCTCCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1045 CTCGAG C^TCGAG XmaCI 221 CCCGGG C^CCGGG XmaI 221 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AspHI 498 GTGCTC G[AT]GC[AT]^C 1042 GAGCTC AviII 74 TGCGCA TGC^GCA 1319 TGCGCA BanI 823 GGCGCC G^G[CT][GA]CC 1312 GGCGCC BbeI 823 GGCGCC GGCGC^C 1312 GGCGCC BbsI 187 GAAGAC GAAGAC(2/6) 1107 GAAGAC BbvCI 123 GCTGAGG CCTCAGC(-5/-2) 1290 GCTGAGG BfaI 337 CTAG C^TAG 1226 CTAG BpuAI 187 GAAGAC GAAGAC(2/6) 1107 GAAGAC BsaAI 1648 TACGTG [CT]AC^GT[GA] 1703 CACGTG BsaWI 104 ACCGGT [AT]^CCGG[AT] 148 TCCGGT BsiEI 522 CGGCCG CG[GA][CT]^CG 892 CGGCCG BsiHKAI 498 GTGCTC G[AT]GC[AT]^C 1042 GAGCTC BssHII 975 GCGCGC G^CGCGC 977 GCGCGC BssSI 747 CTCGTG CACGAG(-5/-1) 1588 CTCGTG BstYI 1169 AGATCT [GA]^GATC[CT] 1232 AGATCC EagI 522 CGGCCG C^GGCCG 892 CGGCCG EclXI 522 CGGCCG C^GGCCG 892 CGGCCG Eco52I 522 CGGCCG C^GGCCG 892 CGGCCG FspI 74 TGCGCA TGC^GCA 1319 TGCGCA KasI 823 GGCGCC G^GCGCC 1312 GGCGCC MaeI 337 CTAG C^TAG 1226 CTAG MaeIII 152 GTCAC ^GT[ACGT]AC 589 GTCAC MflI 1169 AGATCT [GA]^GATC[CT] 1232 AGATCC NarI 823 GGCGCC GG^CGCC 1312 GGCGCC SfaNI 647 GCATC GCATC(5/9) 785 GCATC SfoI 823 GGCGCC GGC^GCC 1312 GGCGCC TaqI 724 TCGA T^CGA 1046 TCGA Tsp45I 152 GTCAC ^GT[GC]AC 589 GTCAC TthHB8I 724 TCGA T^CGA 1046 TCGA XhoII 1169 AGATCT [GA]^GATC[CT] 1232 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI TspRI Van91I