Restriction map for
pFN21AE1706
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvrII BalI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaWI
BseAI BseRI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRV EcoT14I FbaI FseI
HgaI HincII HindII HindIII HinfI
HpaI HphI KpnI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TaqI TfiI Tsp45I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 532 GGCGCC G[GA]^CG[CT]C
AlwNI 232 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 592 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 162 TGCGCA TGC^GCA
BamHI 585 GGATCC G^GATCC
BbeI 532 GGCGCC GGCGC^C
BfaI 575 CTAG C^TAG
BmyI 258 GGGCAC G[AGT]GC[ACT]^C
BpmI 40 CTGGAG CTGGAG(16/14)
BsaHI 532 GGCGCC G[GA]^CG[CT]C
BsaI 346 GGTCTC GGTCTC(1/5)
BsiEI 97 CGGCCG CG[GA][CT]^CG
BsmFI 315 GGGAC GGGAC(10/14)
BsmI 406 GAATGC GAATGC(1/-1)
Bsp1286I 258 GGGCAC G[AGT]GC[ACT]^C
BstAPI 231 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 186 CATCC GGATG(2/0)
DdeI 9 CTTAG C^T[ACGT]AG
DraII 614 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 144 CCGCGG C^C[GA][CT]GG
EagI 97 CGGCCG C^GGCCG
EclXI 97 CGGCCG C^GGCCG
Eco52I 97 CGGCCG C^GGCCG
EcoO109I 614 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 295 GAATTC G^AATTC
EcoT22I 408 ATGCAT ATGCA^T
FokI 186 CATCC GGATG(9/13)
FspI 162 TGCGCA TGC^GCA
Hin1I 532 GGCGCC G[GA]^CG[CT]C
KasI 532 GGCGCC G^GCGCC
KspI 144 CCGCGG CCGC^GG
MaeI 575 CTAG C^TAG
MseI 506 TTAA T^TAA
MslI 300 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 197 GCCGGC GCC^GGC
NarI 532 GGCGCC GG^CGCC
NgoMIV 197 GCCGGC G^CCGGC
NlaIII 472 CATG CATG^
NotI 96 GCGGCCGC GC^GGCCGC
NsiI 408 ATGCAT ATGCA^T
PpuMI 614 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 340 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 229 CTGCAG CTGCA^G
PvuII 166 CAGCTG CAG^CTG
SacII 144 CCGCGG CCGC^GG
SfcI 229 CTGCAG C^T[GA][CT]AG
SfoI 532 GGCGCC GGC^GCC
Tru9I 506 TTAA T^TAA
XmnI 592 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 126 CGCG CG^CG
145 CGCG
AcsI 295 GAATTC [GA]^AATT[CT]
556 AAATTT
AfaI 609 GTAC GT^AC
627 GTAC
AluI 167 AGCT AG^CT
203 AGCT
ApoI 295 GAATTC [GA]^AATT[CT]
556 AAATTT
BanI 259 GGCACC G^G[CT][GA]CC
532 GGCGCC
BglI 38 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
212 GCCGCACCGGC
BsgI 178 CTGCAC GTGCAG(16/14)
253 GTGCAG
BsmAI 476 GAGAC GTCTC(1/5)
347 GTCTC
Bsp1407I 608 TGTACA T^GTACA
626 TGTACA
BsrBI 94 GAGCGG CCGCTC(-3/-3)
133 GAGCGG
BsrFI 197 GCCGGC [GA]^CCGG[CT]
217 ACCGGC
BsrGI 608 TGTACA T^GTACA
626 TGTACA
BstNI 39 CCTGG CC^[AT]GG
536 CCAGG
BstUI 126 CGCG CG^CG
145 CGCG
BstYI 233 AGATCC [GA]^GATC[CT]
585 GGATCC
Cfr10I 197 GCCGGC [GA]^CCGG[CT]
217 ACCGGC
EaeI 97 CGGCCG [CT]^GGCC[GA]
483 TGGCCG
EarI 280 CTCTTC CTCTTC(1/4)
430 GAAGAG
EcoRII 39 CCTGG ^CC[AT]GG
536 CCAGG
FauI 143 CCCGC CCCGC(4/6)
149 GCGGG
HaeII 139 AGCGCC [GA]GCGC^[CT]
532 GGCGCC
Ksp632I 280 CTCTTC CTCTTC(1/4)
430 GAAGAG
MboII 430 GAAGA GAAGA(8/7)
281 TCTTC
MflI 233 AGATCC [GA]^GATC[CT]
585 GGATCC
MvaI 39 CCTGG CC^[AT]GG
536 CCAGG
MvnI 126 CGCG CG^CG
145 CGCG
PflMI 54 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
523 CCAAGAGATGG
RsaI 609 GTAC GT^AC
627 GTAC
SspBI 608 TGTACA T^GTACA
626 TGTACA
Van91I 54 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
523 CCAAGAGATGG
XhoII 233 AGATCC [GA]^GATC[CT]
585 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AlwI AvaII BbvI BcnI
BsaJI BsiYI BslI BspMI BsrI
BssKI Cac8I CfoI Cfr13I DpnII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MnlI MspA1I
MspI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp509I TspRI