Restriction map for
pFN21AB6662
(Linear) (Six-base) MAPSORT of: KIBB6662.seq Check: 538 from: 1 to: 1353
KIBB6662.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 247 1346 1353
Size: 157 90 1099 7
Fragments arranged by size:
1099 157 90 7
AhdI GACnn_n'nnGTC
Cuts at: 0 1156 1353
Size: 1156 197
ApaLI G'TGCA_C
Cuts at: 0 1195 1353
Size: 1195 158
ApoI r'AATT_y
Cuts at: 0 64 426 981 1289 1353
Size: 64 362 555 308 64
Fragments arranged by size:
555 362 308 64 64
AvaI C'yCGr_G
Cuts at: 0 831 1353
Size: 831 522
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1167 1200 1353
Size: 1167 33 153
Fragments arranged by size:
1167 153 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 1168 1353
Size: 1168 185
BclI T'GATC_A
Cuts at: 0 444 1353
Size: 444 909
Bme1580I G_kGCm'C
Cuts at: 0 1199 1353
Size: 1199 154
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1088 1353
Size: 1088 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1353
Size: 881 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 813 1353
Size: 813 540
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1017 1047 1353
Size: 1017 30 306
Fragments arranged by size:
1017 306 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 661 1353
Size: 163 498 692
Fragments arranged by size:
692 498 163
BseYI C'CCAG_C
Cuts at: 0 1133 1353
Size: 1133 220
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1353
Size: 137 1216
BsiEI CG_ry'CG
Cuts at: 0 1131 1353
Size: 1131 222
BsiHKAI G_wGCw'C
Cuts at: 0 1199 1353
Size: 1199 154
BsmI GAATG_Cn'
Cuts at: 0 516 1353
Size: 516 837
Bsp1286I G_dGCh'C
Cuts at: 0 1199 1353
Size: 1199 154
BsrBI CCG'CTC
Cuts at: 0 1067 1353
Size: 1067 286
BsrDI GCAATG_nn'
Cuts at: 0 556 1303 1353
Size: 556 747 50
Fragments arranged by size:
747 556 50
BssSI C'ACGA_G
Cuts at: 0 175 875 1075 1353
Size: 175 700 200 278
Fragments arranged by size:
700 278 200 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 160 1353
Size: 160 1193
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 505 1353
Size: 405 100 848
Fragments arranged by size:
848 405 100
BtsI GCAGTG_nn'
Cuts at: 0 13 1353
Size: 13 1340
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 543 578 1353
Size: 543 35 775
Fragments arranged by size:
775 543 35
EaeI y'GGCC_r
Cuts at: 0 1104 1353
Size: 1104 249
EarI CTCTTCn'nnn_
Cuts at: 0 308 361 1353
Size: 308 53 992
Fragments arranged by size:
992 308 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 247 881 1346 1353
Size: 157 90 634 465 7
Fragments arranged by size:
634 465 157 90 7
EcoO109I rG'GnC_Cy
Cuts at: 0 817 853 898 1353
Size: 817 36 45 455
Fragments arranged by size:
817 455 45 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 61 66 93 429 461 511 543
Size: 34 27 5 27 336 32 50 32
Cuts at: 543 1142 1174 1353
Size: 599 32 179
Fragments arranged by size:
599 336 179 50 34 32 32 32
27 27 5
MfeI C'AATT_G
Cuts at: 0 929 1353
Size: 929 424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1128 1353
Size: 1128 225
MslI CAynn'nnrTG
Cuts at: 0 509 1353
Size: 509 844
MspA1I CmG'CkG
Cuts at: 0 1103 1353
Size: 1103 250
PfoI T'CCnGG_A
Cuts at: 0 822 939 1353
Size: 822 117 414
Fragments arranged by size:
822 414 117
PpuMI rG'GwC_Cy
Cuts at: 0 817 853 898 1353
Size: 817 36 45 455
Fragments arranged by size:
817 455 45 36
PstI C_TGCA'G
Cuts at: 0 473 1353
Size: 473 880
PvuI CG_AT'CG
Cuts at: 0 1131 1353
Size: 1131 222
ScaI AGT'ACT
Cuts at: 0 737 1353
Size: 737 616
SfcI C'TryA_G
Cuts at: 0 469 1161 1353
Size: 469 692 192
Fragments arranged by size:
692 469 192
SmaI CCC'GGG
Cuts at: 0 833 1353
Size: 833 520
SmlI C'TyrA_G
Cuts at: 0 792 1353
Size: 792 561
TatI w'GTAC_w
Cuts at: 0 735 1353
Size: 735 618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1017 1049 1353
Size: 1017 32 304
Fragments arranged by size:
1017 304 32
XmaI C'CCGG_G
Cuts at: 0 831 1353
Size: 831 522
XmnI GAAnn'nnTTC
Cuts at: 0 142 613 1353
Size: 142 471 740
Fragments arranged by size:
740 471 142
Enzymes that do cut:
AcuI AhdI ApaLI ApoI AvaI BaeI BciVI BclI
Bme1580I BplI BpmI BpuEI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BssSI BstAPI
BstXI BtsI CspCI EaeI EarI Eco57MI EcoO109I Hin4I
MfeI MmeI MslI MspA1I PfoI PpuMI PstI PvuI
ScaI SfcI SmaI SmlI TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmBI
BspEI BspHI BspMI BsrFI BsrGI BssHII BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII Tth111I
XbaI XcmI XhoI ZraI