################################## # NO CUT ENZYMES ( 217 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FokI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MluI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AcsI 161 AAATTC [GA]^AATT[CT] AocI 56 CCTCAGG CC^T[ACGT]AGG ApoI 161 AAATTC [GA]^AATT[CT] BalI 84 TGGCCA TGG^CCA BanI 169 GGCACC G^G[CT][GA]CC BbvI 197 GCTGC GCAGC(8/12) BclI 96 TGATCA T^GATCA BglII 91 AGATCT A^GATCT BsaJI 81 CCTTGG C^C[ACGT][ACGT]GG BsmAI 131 GTCTC GTCTC(1/5) BsrDI 126 GCAATG GCAATG(2/0) BstYI 91 AGATCT [GA]^GATC[CT] Bsu36I 56 CCTCAGG CC^T[ACGT]AGG Cac8I 142 GCCTGC GC[ACGT]^[ACGT]GC EaeI 84 TGGCCA [CT]^GGCC[GA] Eco81I 56 CCTCAGG CC^T[ACGT]AGG EcoT14I 81 CCTTGG C^C[AT][AT]GG FbaI 96 TGATCA T^GATCA Fnu4HI 197 GCTGC GC^[ACGT]GC HgaI 110 GCGTC GACGC(5/10) ItaI 197 GCTGC GC^[ACGT]GC MfeI 105 CAATTG C^AATTG MflI 91 AGATCT [GA]^GATC[CT] MluNI 84 TGGCCA TGG^CCA MscI 84 TGGCCA TGG^CCA MunI 105 CAATTG C^AATTG NlaIV 169 GGCACC GG[ACGT]^[ACGT]CC StuI 78 AGGCCT AGG^CCT StyI 81 CCTTGG C^C[AT][AT]GG TseI 197 GCTGC G^C[AT]GC TspRI 117 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 91 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 3 enzymes) # ################################### DdeI 47 CTGAG C^T[ACGT]AG 57 CTCAG MaeIII 65 GTAAC ^GT[ACGT]AC 176 GTTAC Tsp509I 106 AATT ^AATT 162 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### DpnII HaeIII MboI MnlI NdeII Sau3AI