(Circular) (Six-base) MAPSORT of: pFN21AE3001.seq Check: 1468 from: 1 to: 4909 pFN21AE3001 4909 bp With 182 enzymes: SgfI * March 28, 2012 12:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2719 278 Size: 53 83 186 936 1183 2468 Fragments arranged by size: 2468 1183 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2252 1937 Size: 315 4594 Acc65I G'GTAC_C Cuts at: 2230 2230 Size: 4909 AclI AA'CG_TT Cuts at: 1563 3036 3409 1563 Size: 1473 373 3063 Fragments arranged by size: 3063 1473 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2972 4356 4624 1632 Size: 1340 1384 268 1917 Fragments arranged by size: 1917 1384 1340 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4712 AflIII A'CryG_T Cuts at: 1203 3808 4785 1203 Size: 2605 977 1327 Fragments arranged by size: 2605 1327 977 AgeI A'CCGG_T Cuts at: 3740 3740 Size: 4909 AhdI GACnn_n'nnGTC Cuts at: 3638 3638 Size: 4909 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4909 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4909 AlwNI CAG_nnn'CTG Cuts at: 2652 4224 2652 Size: 1572 3337 ApaLI G'TGCA_C Cuts at: 2966 4122 2966 Size: 1156 3753 ApoI r'AATT_y Cuts at: 1493 2166 2218 2444 2596 4705 1493 Size: 673 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 673 226 152 52 AseI AT'TA_AT Cuts at: 160 1032 3463 160 Size: 872 2431 1606 Fragments arranged by size: 2431 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4909 AvaI C'yCGr_G Cuts at: 1870 1943 2234 1870 Size: 73 291 4545 Fragments arranged by size: 4545 291 73 BamHI G'GATC_C Cuts at: 2239 2606 2239 Size: 367 4542 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2230 3685 618 Size: 325 217 627 387 56 1455 1842 Fragments arranged by size: 1842 1455 627 387 325 217 56 BanII G_rGCy'C Cuts at: 1391 2228 1391 Size: 837 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3100 3134 1903 Size: 34 1163 34 3678 Fragments arranged by size: 3678 1163 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2801 4011 2801 Size: 1210 3699 BclI T'GATC_A Cuts at: 1548 2101 1548 Size: 553 4356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3520 136 Size: 107 122 71 631 605 1848 1525 Fragments arranged by size: 1848 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2096 4904 2096 Size: 2808 2101 BlpI GC'TnA_GC Cuts at: 2326 2326 Size: 4909 Bme1580I G_kGCm'C Cuts at: 1790 2970 4126 1790 Size: 1180 1156 2573 Fragments arranged by size: 2573 1180 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2640 3598 442 Size: 416 53 83 399 24 1223 958 1753 Fragments arranged by size: 1753 1223 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4909 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2077 Size: 4909 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3569 1239 Size: 2330 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3034 3899 4197 3034 Size: 865 298 3746 Fragments arranged by size: 3746 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3572 882 Size: 617 2073 2219 Fragments arranged by size: 2219 2073 617 BsaAI yAC'GTr Cuts at: 493 1204 2700 493 Size: 711 1496 2702 Fragments arranged by size: 2702 1496 711 BsaBI GATnn'nnATC Cuts at: 4903 4903 Size: 4909 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2716 3098 3733 275 Size: 53 83 186 936 1183 382 635 1451 Fragments arranged by size: 1451 1183 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3342 3740 4014 4161 4572 3342 Size: 398 274 147 411 3679 Fragments arranged by size: 3679 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4684 Fragments arranged by size: 4684 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4909 BseYI C'CCAG_C Cuts at: 4112 4608 4112 Size: 496 4413 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2359 3120 4148 1540 Size: 275 186 358 761 1028 2301 Fragments arranged by size: 2301 1028 761 358 275 186 BsiHKAI G_wGCw'C Cuts at: 2970 3055 4126 2970 Size: 85 1071 3753 Fragments arranged by size: 3753 1071 85 BsmI GAATG_Cn' Cuts at: 2420 2513 2420 Size: 93 4816 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2970 3055 4126 1391 Size: 399 438 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 438 399 85 BspEI T'CCGG_A Cuts at: 4572 4572 Size: 4909 BspHI T'CATG_A Cuts at: 2798 4528 2798 Size: 1730 3179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2264 844 Size: 1420 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2796 1394 Size: 429 973 3507 Fragments arranged by size: 3507 973 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2138 3404 3578 65 Size: 1183 890 1266 174 1396 Fragments arranged by size: 1396 1266 1183 890 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3553 3740 1700 Size: 110 1743 187 2869 Fragments arranged by size: 2869 1743 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4909 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4909 BssSI C'ACGA_G Cuts at: 2969 3981 2969 Size: 1012 3897 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4909 BstBI TT'CG_AA Cuts at: 3713 3713 Size: 4909 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4909 BstYI r'GATC_y Cuts at: 1972 2096 2239 2606 2991 3008 4460 4546 4558 Size: 124 143 367 385 17 1452 86 12 Cuts at: 4558 4904 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 143 124 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2062 2062 Size: 4909 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4357 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4170 Fragments arranged by size: 4170 683 56 BtsI GCAGTG_nn' Cuts at: 2128 2425 3219 3239 2128 Size: 297 794 20 3798 Fragments arranged by size: 3798 794 297 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4909 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4874 DraI TTT'AAA Cuts at: 2213 2213 Size: 4909 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3916 809 Size: 507 2600 1802 Fragments arranged by size: 2600 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2356 3245 Size: 54 1425 50 275 18 259 267 889 Cuts at: 3245 4874 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 275 267 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2356 1537 Size: 275 544 4090 Fragments arranged by size: 4090 544 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2839 1353 Size: 1486 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3496 3870 4016 387 Size: 1064 138 1907 374 146 1280 Fragments arranged by size: 1907 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2972 3569 4356 4624 1239 Size: 393 1340 597 787 268 1524 Fragments arranged by size: 1524 1340 787 597 393 268 EcoRI G'AATT_C Cuts at: 2218 2596 2218 Size: 378 4531 FspI TGC'GCA Cuts at: 3415 3415 Size: 4909 HaeII r_GCGC'y Cuts at: 1678 4056 1678 Size: 2378 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3550 3582 3624 3656 1373 Size: 32 135 32 1978 32 42 32 2626 Fragments arranged by size: 2626 1978 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2253 2499 669 Size: 988 281 315 246 3079 Fragments arranged by size: 3079 988 315 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4909 HpaI GTT'AAC Cuts at: 2499 2499 Size: 4909 KpnI G_GTAC'C Cuts at: 2234 2234 Size: 4909 MfeI C'AATT_G Cuts at: 2110 2508 2110 Size: 398 4511 MluI A'CGCG_T Cuts at: 4785 4785 Size: 4909 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4023 4207 1305 Size: 2718 184 2007 Fragments arranged by size: 2718 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2091 10 Size: 54 1425 343 259 2828 Fragments arranged by size: 2828 1425 343 259 54 MslI CAynn'nnrTG Cuts at: 518 1166 2867 3226 3385 518 Size: 648 1701 359 159 2042 Fragments arranged by size: 2042 1701 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2325 3002 4150 4395 1606 Size: 719 677 1148 245 2120 Fragments arranged by size: 2120 1148 719 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4357 NdeI CA'TA_TG Cuts at: 387 387 Size: 4909 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4909 NotI GC'GGCC_GC Cuts at: 2356 2356 Size: 4909 NspI r_CATG'y Cuts at: 1132 1229 2267 3812 1132 Size: 97 1038 1545 2229 Fragments arranged by size: 2229 1545 1038 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4909 PciI A'CATG_T Cuts at: 3808 3808 Size: 4909 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4909 PmeI GTTT'AAAC Cuts at: 2213 2213 Size: 4909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2978 3010 4491 4523 2978 Size: 32 1481 32 3364 Fragments arranged by size: 3364 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4909 PsiI TTA'TAA Cuts at: 2479 2479 Size: 4909 PstI C_TGCA'G Cuts at: 830 2261 830 Size: 1431 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4909 SalI G'TCGA_C Cuts at: 1936 2251 1936 Size: 315 4594 SbfI CC_TGCA'GG Cuts at: 2261 2261 Size: 4909 ScaI AGT'ACT Cuts at: 3157 4869 3157 Size: 1712 3197 SfcI C'TryA_G Cuts at: 826 1045 2257 3392 4073 4264 826 Size: 219 1212 1135 681 191 1471 Fragments arranged by size: 1471 1212 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4909 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4909 SmaI CCC'GGG Cuts at: 1872 2236 1872 Size: 364 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3013 3914 4176 820 Size: 197 926 1070 901 262 1553 Fragments arranged by size: 1553 1070 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2700 493 Size: 2207 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4909 SphI G_CATG'C Cuts at: 1132 2267 1132 Size: 1135 3774 SspI AAT'ATT Cuts at: 5 52 2833 5 Size: 47 2781 2081 Fragments arranged by size: 2781 2081 47 StuI AGG'CCT Cuts at: 1517 2085 1517 Size: 568 4341 StyI C'CwwG_G Cuts at: 513 1065 2086 2348 513 Size: 552 1021 262 3074 Fragments arranged by size: 3074 1021 552 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2936 2953 3106 3291 3736 325 Size: 1255 1356 17 153 185 445 1498 Fragments arranged by size: 1498 1356 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3155 4867 96 Size: 274 80 33 51 1443 1178 1712 138 Fragments arranged by size: 1712 1443 1178 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4869 4901 4869 Size: 32 4877 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4909 XbaI T'CTAG_A Cuts at: 2245 2245 Size: 4909 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4909 XmaI C'CCGG_G Cuts at: 1870 2234 1870 Size: 364 4545 XmnI GAAnn'nnTTC Cuts at: 3038 3038 Size: 4909 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2717 276 Size: 53 83 186 936 1183 2468 Fragments arranged by size: 2468 1183 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI