################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I FbaI FseI FspI HaeII Hin1I KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI NarI NciI NcoI NdeI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 669 AAATTC [GA]^AATT[CT] AlwI 1470 GATCC GGATC(4/5) ApoI 669 AAATTC [GA]^AATT[CT] Asp700I 561 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 595 GAAGAC GAAGAC(2/6) BciVI 658 GTATCC GTATCC(6/5) BpuAI 595 GAAGAC GAAGAC(2/6) BsaJI 710 CCACGG C^C[ACGT][ACGT]GG BseRI 953 GAGGAG GAGGAG(10/8) BsiYI 399 CCAAACAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 399 CCAAACAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 342 GAATGC GAATGC(1/-1) BsrFI 1228 GCCGGC [GA]^CCGG[CT] BssSI 459 CACGAG CACGAG(-5/-1) BstAPI 231 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1039 GGTAACC G^GT[ACGT]ACC BstPI 1039 GGTAACC G^GT[ACGT]ACC CfoI 205 GCGC GCG^C Cfr10I 1228 GCCGGC [GA]^CCGG[CT] DraIII 270 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 777 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 710 CCACGG C^C[GA][CT]GG EaeI 44 TGGCCG [CT]^GGCC[GA] EcoO65I 1039 GGTAACC G^GT[ACGT]ACC EcoT22I 1343 ATGCAT ATGCA^T FauI 959 GCGGG CCCGC(4/6) HapII 1229 CCGG C^CGG HgaI 202 GACGC GACGC(5/10) HhaI 205 GCGC GCG^C HinP1I 205 GCGC G^CGC HindIII 286 AAGCTT A^AGCTT HpaII 1229 CCGG C^CGG MslI 1458 CACAACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 404 CAGCGG C[AC]G^C[GT]G MspI 1229 CCGG C^CGG NaeI 1228 GCCGGC GCC^GGC NgoMIV 1228 GCCGGC G^CCGGC NheI 585 GCTAGC G^CTAGC NsiI 1343 ATGCAT ATGCA^T NspI 1345 GCATGT [GA]CATG^[CT] PleI 223 GACTC GAGTC(4/5) PstI 120 CTGCAG CTGCA^G SspI 1055 AATATT AAT^ATT StuI 929 AGGCCT AGG^CCT TfiI 1200 GATTC G^A[AT]TC XmnI 561 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 204 CGCG CG^CG 416 CGCG AflIII 179 ACGTGT A^C[GA][CT]GT 893 ACACGT AlwNI 232 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 843 CAGAGACTG BsaAI 178 TACGTG [CT]AC^GT[GA] 1105 TACGTG BsgI 193 GTGCAG GTGCAG(16/14) 229 GTGCAG BsrBI 159 CCGCTC CCGCTC(-3/-3) 208 CCGCTC BssKI 303 CCAGG ^CC[ACGT]GG 1255 CCAGG BstNI 303 CCAGG CC^[AT]GG 1255 CCAGG BstUI 204 CGCG CG^CG 416 CGCG Cac8I 585 GCTAGC GC[ACGT]^[ACGT]GC 1228 GCCGGC DraII 115 GGGTCCT [GA]G^G[ACGT]CC[CT] 806 AGGACCC EcoO109I 115 GGGTCCT [GA]G^G[ACGT]CC[CT] 806 AGGACCC EcoRII 303 CCAGG ^CC[AT]GG 1255 CCAGG HincII 423 GTTAAC GT[CT]^[GA]AC 897 GTTAAC HindII 423 GTTAAC GT[CT]^[GA]AC 897 GTTAAC HinfI 223 GACTC G^A[ACGT]TC 1200 GATTC HpaI 423 GTTAAC GTT^AAC 897 GTTAAC HphI 714 GGTGA GGTGA(8/7) 1203 TCACC MvaI 303 CCAGG CC^[AT]GG 1255 CCAGG MvnI 204 CGCG CG^CG 416 CGCG NlaIV 115 GGGTCC GG[ACGT]^[ACGT]CC 807 GGACCC PpuMI 115 GGGTCCT [GA]G^G[AT]CC[CT] 806 AGGACCC SapI 69 GAAGAGC GCTCTTC(1/4) 161 GCTCTTC ScrFI 303 CCAGG CC^[ACGT]GG 1255 CCAGG TaqI 212 TCGA T^CGA 496 TCGA Tsp45I 703 GTGAC ^GT[GC]AC 938 GTGAC TthHB8I 212 TCGA T^CGA 496 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BfaI BpmI BsmAI BsmFI BsrI BstF5I Cfr13I DdeI DpnII EarI Fnu4HI FokI HaeIII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII RsaI Sau3AI Sau96I SfaNI SfcI SmlI Tru9I TseI Tsp509I TspRI