(Circular) (Six-base) MAPSORT of: pFN21AE3220.seq Check: 5085 from: 1 to: 6193 pFN21AE3220 6193 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2462 2830 2880 3036 4256 5640 5908 1632 Size: 830 368 50 156 1220 1384 268 1917 Fragments arranged by size: 1917 1384 1220 830 368 268 156 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2184 2898 5092 6069 1203 Size: 981 714 2194 977 1327 Fragments arranged by size: 2194 1327 981 977 714 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6193 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AlwNI CAG_nnn'CTG Cuts at: 2242 2853 3936 5508 2242 Size: 611 1083 1572 2927 Fragments arranged by size: 2927 1572 1083 611 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2674 3502 3728 3880 5989 1493 Size: 1181 828 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 828 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BamHI G'GATC_C Cuts at: 3523 3890 3523 Size: 367 5826 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3514 4969 618 Size: 325 217 627 1727 1455 1842 Fragments arranged by size: 1842 1727 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3512 1391 Size: 2121 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2607 928 Size: 971 708 4514 Fragments arranged by size: 4514 971 708 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2674 4085 5295 2674 Size: 1411 1210 3572 Fragments arranged by size: 3572 1411 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BfrBI ATG'CAT Cuts at: 3350 3350 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 4254 5410 1790 Size: 2464 1156 2573 Fragments arranged by size: 2573 2464 1156 BmgBI CAC'GTC Cuts at: 1318 2987 1318 Size: 1669 4524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3145 3924 4882 Size: 416 53 83 399 24 1728 779 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1728 958 779 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2594 1056 Size: 1538 4655 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3172 3172 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 2273 2976 4853 1239 Size: 919 115 703 1877 2579 Fragments arranged by size: 2579 1877 919 703 115 Bpu10I CC'TnA_GC Cuts at: 2808 3442 2808 Size: 634 5559 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2315 3166 3440 4318 5183 5481 2315 Size: 851 274 878 865 298 3027 Fragments arranged by size: 3027 878 865 851 298 274 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4856 882 Size: 617 3357 2219 Fragments arranged by size: 3357 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2185 3112 3984 493 Size: 711 981 927 872 2702 Fragments arranged by size: 2702 981 927 872 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4000 4382 5017 275 Size: 53 83 186 936 2467 382 635 1451 Fragments arranged by size: 2467 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5968 Fragments arranged by size: 5968 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2973 1186 Size: 1787 4406 BseYI C'CCAG_C Cuts at: 3067 5396 5892 3067 Size: 2329 496 3368 Fragments arranged by size: 3368 2329 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2255 1163 Size: 573 483 36 5101 Fragments arranged by size: 5101 573 483 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4254 4339 5410 4254 Size: 85 1071 5037 Fragments arranged by size: 5037 1071 85 BsmI GAATG_Cn' Cuts at: 2353 3704 3797 2353 Size: 1351 93 4749 Fragments arranged by size: 4749 1351 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3512 4254 4339 5410 1391 Size: 399 1722 742 85 1071 2174 Fragments arranged by size: 2174 1722 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3548 844 Size: 2704 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2166 2215 4080 1394 Size: 429 343 49 1865 3507 Fragments arranged by size: 3507 1865 429 343 49 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3233 4837 5024 1700 Size: 110 1423 1604 187 2869 Fragments arranged by size: 2869 1604 1423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 2464 4253 5265 2464 Size: 1789 1012 3392 Fragments arranged by size: 3392 1789 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 4745 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 3044 1184 Size: 1860 4333 BstYI r'GATC_y Cuts at: 1972 3523 3890 4275 4292 5744 5830 5842 6188 Size: 1551 367 385 17 1452 86 12 346 Cuts at: 6188 1972 Size: 1977 Fragments arranged by size: 1977 1551 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2715 513 Size: 552 1650 3991 Fragments arranged by size: 3991 1650 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 3709 4503 4523 3709 Size: 794 20 5379 Fragments arranged by size: 5379 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2452 2487 529 Size: 35 1888 35 4235 Fragments arranged by size: 4235 1888 35 35 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DraIII CAC_nnn'GTG Cuts at: 2280 2280 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2788 5200 809 Size: 507 1472 2412 1802 Fragments arranged by size: 2412 1802 1472 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 3640 4529 Size: 54 1425 50 275 18 219 1591 889 Cuts at: 4529 6158 8 Size: 1629 43 Fragments arranged by size: 1629 1591 1425 889 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2069 2173 2631 2919 4123 1353 Size: 716 104 458 288 1204 3423 Fragments arranged by size: 3423 1204 716 458 288 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2273 2462 2830 2880 2976 3036 Size: 393 526 115 189 368 50 96 60 Cuts at: 3036 4256 4853 5640 5908 1239 Size: 1220 597 787 268 1524 Fragments arranged by size: 1524 1220 787 597 526 393 368 268 189 115 96 60 50 EcoO109I rG'GnC_Cy Cuts at: 2121 2812 2121 Size: 691 5502 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 5340 1678 Size: 3662 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2023 2055 4834 4866 4908 Size: 32 135 32 451 32 2779 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2779 2626 451 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2430 2904 3537 3783 669 Size: 988 281 492 474 633 246 3079 Fragments arranged by size: 3079 988 633 492 474 281 246 HindIII A'AGCT_T Cuts at: 748 2291 748 Size: 1543 4650 HpaI GTT'AAC Cuts at: 2430 2904 3783 2430 Size: 474 879 4840 Fragments arranged by size: 4840 879 474 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2641 3387 3502 5307 5491 1305 Size: 1336 746 115 1805 184 2007 Fragments arranged by size: 2007 1805 1336 746 184 115 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3467 4151 4510 4669 518 Size: 648 2301 684 359 159 2042 Fragments arranged by size: 2301 2042 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2411 3609 4286 5434 5679 1606 Size: 805 1198 677 1148 245 2120 Fragments arranged by size: 2120 1198 1148 805 677 245 NaeI GCC'GGC Cuts at: 3235 3235 Size: 6193 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5641 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 3233 3233 Size: 6193 NheI G'CTAG_C Cuts at: 1052 2590 1052 Size: 1538 4655 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NsiI A_TGCA'T Cuts at: 3352 3352 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3354 3551 5096 1132 Size: 97 2125 197 1545 2229 Fragments arranged by size: 2229 2125 1545 197 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6193 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PfoI T'CCnGG_A Cuts at: 2307 2307 Size: 6193 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2466 2481 2498 2513 4262 4294 5775 5807 2466 Size: 15 17 15 1749 32 1481 32 2852 Fragments arranged by size: 2852 1749 1481 32 32 17 15 15 PpuMI rG'GwC_Cy Cuts at: 2121 2812 2121 Size: 691 5502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3362 3394 3362 Size: 32 6161 PstI C_TGCA'G Cuts at: 830 2129 3545 830 Size: 1299 1416 3478 Fragments arranged by size: 3478 1416 1299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SapI GCTCTTCn'nnn_ Cuts at: 2069 2173 2069 Size: 104 6089 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SfcI C'TryA_G Cuts at: 826 1045 2125 2621 2651 2919 3541 4676 5357 Size: 219 1080 496 30 268 622 1135 681 Cuts at: 5357 5548 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1080 681 622 496 268 219 191 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 3181 3455 4297 5198 5460 Size: 197 926 351 887 274 842 901 262 Cuts at: 5460 820 Size: 1553 Fragments arranged by size: 1553 926 901 887 842 351 274 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 3062 4117 5 Size: 47 3010 1055 2081 Fragments arranged by size: 3010 2081 1055 47 StuI AGG'CCT Cuts at: 1517 2936 1517 Size: 1419 4774 StyI C'CwwG_G Cuts at: 513 1065 3632 513 Size: 552 2567 3074 Fragments arranged by size: 3074 2567 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4220 4237 4390 4575 5020 325 Size: 1255 2640 17 153 185 445 1498 Fragments arranged by size: 2640 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2353 3378 4439 Size: 274 80 33 51 1443 376 1025 1061 Cuts at: 4439 6151 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1061 1025 376 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 2570 4322 2570 Size: 1752 4441 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI