################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeIII MamI MfeI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 383 TCCGGA T^CCGGA AcyI 109 GGCGTC G[GA]^CG[CT]C AseI 733 ATTAAT AT^TAAT AsnI 733 ATTAAT AT^TAAT Asp700I 180 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 65 GGATCC G^GATCC BanII 248 GGGCTC G[GA]GC[CT]^C BfaI 475 CTAG C^TAG BglI 277 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 496 CTGGAG CTGGAG(16/14) BsaHI 109 GGCGTC G[GA]^CG[CT]C BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG BseAI 383 TCCGGA T^CCGGA BsgI 209 GTGCAG GTGCAG(16/14) BsmAI 558 GTCTC GTCTC(1/5) BsmI 147 GAATGC GAATGC(1/-1) BspEI 383 TCCGGA T^CCGGA BspMI 517 ACCTGC ACCTGC(4/8) BsrBI 69 CCGCTC CCGCTC(-3/-3) BstAPI 834 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 74 CCACGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 65 GGATCC [GA]^GATC[CT] CfoI 286 GCGC GCG^C DdeI 761 CTCAG C^T[ACGT]AG DrdI 658 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 53 ATGCAT ATGCA^T FauI 544 GCGGG CCCGC(4/6) HgaI 110 GCGTC GACGC(5/10) HhaI 286 GCGC GCG^C Hin1I 109 GGCGTC G[GA]^CG[CT]C HinP1I 286 GCGC G^CGC MaeI 475 CTAG C^TAG MaeII 487 ACGT A^CGT MflI 65 GGATCC [GA]^GATC[CT] MluI 205 ACGCGT A^CGCGT MroI 383 TCCGGA T^CCGGA MslI 75 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NsiI 53 ATGCAT ATGCA^T PshBI 733 ATTAAT AT^TAAT SphI 273 GCATGC GCATG^C StuI 240 AGGCCT AGG^CCT XcmI 485 CCACGTTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 65 GGATCC [GA]^GATC[CT] XmnI 180 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 206 CGCG CG^CG 287 CGCG AlwI 66 GATCC GGATC(4/5) 65 GGATC AlwNI 580 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 835 CAGCTGCTG AspHI 228 GAGCAC G[AT]GC[AT]^C 504 GAGCAC BsaWI 383 TCCGGA [AT]^CCGG[AT] 777 TCCGGT BsiHKAI 228 GAGCAC G[AT]GC[AT]^C 504 GAGCAC BspLU11I 596 ACATGT A^CATGT 629 ACATGT BstF5I 56 CATCC GGATG(2/0) 530 GGATG BstUI 206 CGCG CG^CG 287 CGCG DpnII 66 GATC ^GATC 378 GATC DraII 191 GGGACCT [GA]G^G[ACGT]CC[CT] 260 GGGACCC EarI 138 CTCTTC CTCTTC(1/4) 849 GAAGAG EcoO109I 191 GGGACCT [GA]G^G[ACGT]CC[CT] 260 GGGACCC FokI 56 CATCC GGATG(9/13) 530 GGATG HapII 384 CCGG C^CGG 778 CCGG HpaII 384 CCGG C^CGG 778 CCGG Ksp632I 138 CTCTTC CTCTTC(1/4) 849 GAAGAG MboI 66 GATC ^GATC 378 GATC MseI 538 TTAA T^TAA 734 TTAA MspA1I 130 CAGCTG C[AC]G^C[GT]G 835 CAGCTG MspI 384 CCGG C^CGG 778 CCGG MvnI 206 CGCG CG^CG 287 CGCG NdeII 66 GATC ^GATC 378 GATC PleI 759 GACTC GAGTC(4/5) 556 GAGTC PpuMI 191 GGGACCT [GA]G^G[AT]CC[CT] 260 GGGACCC PvuII 130 CAGCTG CAG^CTG 835 CAGCTG Sau3AI 66 GATC ^GATC 378 GATC SfaNI 531 GATGC GCATC(5/9) 55 GCATC Tru9I 538 TTAA T^TAA 734 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AflIII AluI AvaII BalI BbsI BbvI BmyI BpuAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I EaeI EcoRII Fnu4HI HaeIII HinfI ItaI MboII MluNI MnlI MscI MvaI MwoI NlaIII NlaIV NspI Sau96I ScrFI TfiI TseI TspRI