################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccII 475 CGCG CG^CG AciI 474 CCGC CCGC(-3/-1) AfaI 354 GTAC GT^AC AlwNI 694 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AspI 472 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 606 CTCGAG C^[CT]CG[GA]G AvaII 32 GGACC G^G[AT]CC BcgI 36 CGAGCTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaJI 333 CCTGGG C^C[ACGT][ACGT]GG BseRI 485 GAGGAG GAGGAG(10/8) BsmFI 31 GGGAC GGGAC(10/14) BsoBI 606 CTCGAG C^[CT]CG[GA]G Bst1107I 194 GTATAC GTA^TAC BstF5I 713 GGATG GGATG(2/0) BstUI 475 CGCG CG^CG BstZ17I 194 GTATAC GTA^TAC EcoNI 11 CCTTCCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 713 GGATG GGATG(9/13) HapII 270 CCGG C^CGG HgaI 476 GCGTC GACGC(5/10) HindIII 544 AAGCTT A^AGCTT HpaII 270 CCGG C^CGG MaeII 771 ACGT A^CGT MspI 270 CCGG C^CGG MvnI 475 CGCG CG^CG MwoI 359 GCAGTAGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 31 GGGACC GG[ACGT]^[ACGT]CC PaeR7I 606 CTCGAG C^TCGAG PflFI 472 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 357 CTGCAG CTGCA^G RsaI 354 GTAC GT^AC Tsp45I 416 GTCAC ^GT[GC]AC TspRI 532 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 472 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 606 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 194 GTATAC GT^[AC][GT]AC 510 GTCTAC AcsI 133 GAATTT [GA]^AATT[CT] 832 GAATTT ApoI 133 GAATTT [GA]^AATT[CT] 832 GAATTT BanII 37 GAGCTC G[GA]GC[CT]^C 609 GAGCTC BbvI 693 GCAGC GCAGC(8/12) 746 GCAGC BfaI 274 CTAG C^TAG 397 CTAG BsiYI 11 CCTTCCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 112 CCTGTGCATGG BslI 11 CCTTCCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 112 CCTGTGCATGG BsrI 464 ACTGG ACTGG(1/-1) 699 ACTGG Cfr13I 32 GGACC G^G[ACGT]CC 142 GGCCC DraI 777 TTTAAA TTT^AAA 792 TTTAAA Fnu4HI 693 GCAGC GC^[ACGT]GC 746 GCAGC HaeIII 142 GGCC GG^CC 217 GGCC ItaI 693 GCAGC GC^[ACGT]GC 746 GCAGC MaeI 274 CTAG C^TAG 397 CTAG MseI 778 TTAA T^TAA 793 TTAA PleI 25 GAGTC GAGTC(4/5) 508 GAGTC SacI 37 GAGCTC GAGCT^C 609 GAGCTC Sau96I 32 GGACC G^G[ACGT]CC 142 GGCCC SfcI 357 CTGCAG C^T[GA][CT]AG 666 CTACAG SmlI 286 CTTGAG C^T[CT][GA]AG 606 CTCGAG TaqI 607 TCGA T^CGA 639 TCGA TfiI 106 GATTC G^A[AT]TC 736 GATTC Tru9I 778 TTAA T^TAA 793 TTAA TseI 693 GCAGC G^C[AT]GC 746 GCAGC TthHB8I 607 TCGA T^CGA 639 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI AspHI BmyI BsiHKAI Bsp1286I BssKI BstNI DdeI EcoRII HinfI HphI MaeIII MboII MnlI MvaI NlaIII ScrFI Tsp509I