################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AccI AclI AcsI AflII AgeI ApoI AscI AseI AsnI AspI AvrII BclI BfrI BlnI BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRI EcoRV EcoT22I FbaI HindIII HpaI KspI MamI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 3430 GACGTC GACGT^C Acc65I 2201 GGTACC G^GTACC AccIII 1625 TCCGGA T^CCGGA AflIII 3329 ACATGT A^C[GA][CT]GT Aor51HI 698 AGCGCT AGC^GCT Asp718I 2201 GGTACC G^GTACC AviII 3491 TGCGCA TGC^GCA BalI 1496 TGGCCA TGG^CCA BbeI 2463 GGCGCC GGCGC^C BcgI 1076 GCACTGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1155 GGATAC GTATCC(6/5) BfaI 1234 CTAG C^TAG BglII 1511 AGATCT A^GATCT BseAI 1625 TCCGGA T^CCGGA BsmBI 1690 GAGACG CGTCTC(1/5) BsmI 821 GCATTC GAATGC(1/-1) BspEI 1625 TCCGGA T^CCGGA BspHI 1280 TCATGA T^CATGA BspLU11I 3329 ACATGT A^CATGT BsrDI 3051 CATTGC GCAATG(2/0) BstEII 1707 GGTAACC G^GT[ACGT]ACC BstPI 1707 GGTAACC G^GT[ACGT]ACC DraI 1486 TTTAAA TTT^AAA DraIII 3301 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1164 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 698 AGCGCT AGC^GCT EcoO65I 1707 GGTAACC G^GT[ACGT]ACC FseI 331 GGCCGGCC GGCCGG^CC FspI 3491 TGCGCA TGC^GCA HincII 1393 GTCAAC GT[CT]^[GA]AC HindII 1393 GTCAAC GT[CT]^[GA]AC KasI 2463 GGCGCC G^GCGCC KpnI 2201 GGTACC GGTAC^C MaeI 1234 CTAG C^TAG MluNI 1496 TGGCCA TGG^CCA MroI 1625 TCCGGA T^CCGGA MscI 1496 TGGCCA TGG^CCA NarI 2463 GGCGCC GG^CGCC NcoI 3353 CCATGG C^CATGG NheI 1233 GCTAGC G^CTAGC PaeR7I 3316 CTCGAG C^TCGAG PflMI 3040 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1280 TCATGA T^CATGA ScaI 398 AGTACT AGT^ACT SfoI 2463 GGCGCC GGC^GCC SgrAI 2459 CGCCGGCG C[GA]^CCGG[CT]G Tsp509I 1026 AATT ^AATT Van91I 3040 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 3316 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 2463 GGCGCC G[GA]^CG[CT]C 3430 GACGTC AhdI 598 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1669 GACGTGGTGTC AocI 1932 CCTCAGG CC^T[ACGT]AGG 3273 CCTGAGG Asp700I 2268 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2709 GAAGTGGTTC AspEI 598 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1669 GACGTGGTGTC BbrPI 2254 CACGTG CAC^GTG 2931 CACGTG BbsI 1479 GAAGAC GAAGAC(2/6) 3469 GAAGAC BpuAI 1479 GAAGAC GAAGAC(2/6) 3469 GAAGAC BsaHI 2463 GGCGCC G[GA]^CG[CT]C 3430 GACGTC BsaWI 1625 TCCGGA [AT]^CCGG[AT] 2353 ACCGGA Bsu36I 1932 CCTCAGG CC^T[ACGT]AGG 3273 CCTGAGG EagI 1584 CGGCCG C^GGCCG 1804 CGGCCG Eam1105I 598 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1669 GACGTGGTGTC EclXI 1584 CGGCCG C^GGCCG 1804 CGGCCG Eco52I 1584 CGGCCG C^GGCCG 1804 CGGCCG Eco81I 1932 CCTCAGG CC^T[ACGT]AGG 3273 CCTGAGG Hin1I 2463 GGCGCC G[GA]^CG[CT]C 3430 GACGTC MseI 413 TTAA T^TAA 1487 TTAA NspI 1013 ACATGC [GA]CATG^[CT] 3329 ACATGT PmaCI 2254 CACGTG CAC^GTG 2931 CACGTG PmlI 2254 CACGTG CAC^GTG 2931 CACGTG SacI 165 GAGCTC GAGCT^C 814 GAGCTC SapI 12 GAAGAGC GCTCTTC(1/4) 2644 GAAGAGC StuI 527 AGGCCT AGG^CCT 2078 AGGCCT TfiI 725 GAATC G^A[AT]TC 3068 GATTC Tru9I 413 TTAA T^TAA 1487 TTAA XcmI 1010 CCAACATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2468 CCACTGAGCCCGTGG XmnI 2268 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2709 GAAGTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI AspHI AvaI AvaII BamHI BanI BanII BbvCI BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI