(Circular) (Six-base) MAPSORT of: pFN21AE5812.seq Check: 8560 from: 1 to: 8314 pFN21AE5812 8314 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2575 2575 Size: 8314 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5439 6124 278 Size: 53 83 186 936 3903 685 2468 Fragments arranged by size: 3903 2468 936 685 186 83 53 AccI GT'mk_AC Cuts at: 1937 5657 1937 Size: 3720 4594 Acc65I G'GTAC_C Cuts at: 4206 5635 4206 Size: 1429 6885 AclI AA'CG_TT Cuts at: 1563 6441 6814 1563 Size: 4878 373 3063 Fragments arranged by size: 4878 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3046 4159 6377 7761 8029 1632 Size: 1414 1113 2218 1384 268 1917 Fragments arranged by size: 2218 1917 1414 1384 1113 268 AfeI AGC'GCT Cuts at: 2705 2705 Size: 8314 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8117 AflIII A'CryG_T Cuts at: 1203 5334 7213 8190 1203 Size: 4131 1879 977 1327 Fragments arranged by size: 4131 1879 1327 977 AgeI A'CCGG_T Cuts at: 7145 7145 Size: 8314 AhdI GACnn_n'nnGTC Cuts at: 2608 3679 7043 2608 Size: 1071 3364 3879 Fragments arranged by size: 3879 3364 1071 AleI CACnn'nnGTG Cuts at: 1166 3254 4401 1166 Size: 2088 1147 5079 Fragments arranged by size: 5079 2088 1147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5129 1184 Size: 3945 4369 AlwNI CAG_nnn'CTG Cuts at: 2374 3069 3616 3945 4243 4672 5051 6057 7629 Size: 695 547 329 298 429 379 1006 1572 Cuts at: 7629 2374 Size: 3059 Fragments arranged by size: 3059 1572 1006 695 547 429 379 329 298 ApaI G_GGCC'C Cuts at: 2096 2128 2202 2283 3598 3928 4102 4913 2096 Size: 32 74 81 1315 330 174 811 5497 Fragments arranged by size: 5497 1315 811 330 174 81 74 32 ApaLI G'TGCA_C Cuts at: 4262 4561 5303 6371 7527 4262 Size: 299 742 1068 1156 5049 Fragments arranged by size: 5049 1156 1068 742 299 ApoI r'AATT_y Cuts at: 1493 5623 5849 6001 8110 1493 Size: 4130 226 152 2109 1697 Fragments arranged by size: 4130 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6868 160 Size: 872 5836 1606 Fragments arranged by size: 5836 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 AvaI C'yCGr_G Cuts at: 1870 1943 2167 2385 2598 2924 3361 3648 3966 Size: 73 224 218 213 326 437 287 318 Cuts at: 3966 4164 5238 5321 5639 1870 Size: 198 1074 83 318 4545 Fragments arranged by size: 4545 1074 437 326 318 318 287 224 218 213 198 83 73 BamHI G'GATC_C Cuts at: 2565 3736 4690 5644 6011 2565 Size: 1171 954 954 367 4868 Fragments arranged by size: 4868 1171 954 954 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2486 2941 3464 3932 4206 Size: 325 217 627 699 455 523 468 274 Cuts at: 4206 4468 4837 5635 7090 618 Size: 262 369 798 1455 1842 Fragments arranged by size: 1842 1455 798 699 627 523 468 455 369 325 274 262 217 BanII G_rGCy'C Cuts at: 1391 2096 2118 2128 2174 2202 2283 2309 2823 Size: 705 22 10 46 28 81 26 514 Cuts at: 2823 2901 3598 3928 3960 4102 4482 4656 4913 Size: 78 697 330 32 142 380 174 257 Cuts at: 4913 4993 5490 5633 1391 Size: 80 497 143 4072 Fragments arranged by size: 4072 705 697 514 497 380 330 257 174 143 142 81 80 78 46 32 28 26 22 10 BbeI G_GCGC'C Cuts at: 4472 4472 Size: 8314 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3491 5481 928 Size: 971 1592 1990 3761 Fragments arranged by size: 3761 1990 1592 971 BbvCI CC'TCA_GC Cuts at: 2255 4617 4682 2255 Size: 2362 65 5887 Fragments arranged by size: 5887 2362 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3070 3104 6505 6539 1903 Size: 34 1133 34 3401 34 3678 Fragments arranged by size: 3678 3401 1133 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3154 6206 7416 3154 Size: 3052 1210 4052 Fragments arranged by size: 4052 3052 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2105 2474 3005 Size: 107 122 71 631 605 433 369 531 Cuts at: 3005 3275 4115 4420 4518 6925 136 Size: 270 840 305 98 2407 1525 Fragments arranged by size: 2407 1525 840 631 605 531 433 369 305 270 122 107 98 71 BglII A'GATC_T Cuts at: 3516 8309 3516 Size: 4793 3521 BlpI GC'TnA_GC Cuts at: 2526 4214 4445 4634 4943 5731 2526 Size: 1688 231 189 309 788 5109 Fragments arranged by size: 5109 1688 788 309 231 189 Bme1580I G_kGCm'C Cuts at: 1790 2096 2128 2202 2283 2946 3354 3467 3539 Size: 306 32 74 81 663 408 113 72 Cuts at: 3539 3598 3928 3935 4102 4266 4565 4913 4974 Size: 59 330 7 167 164 299 348 61 Cuts at: 4974 5307 5574 6375 7531 1790 Size: 333 267 801 1156 2573 Fragments arranged by size: 2573 1156 801 663 408 348 333 330 306 299 267 167 164 113 81 74 72 61 59 32 7 BmgBI CAC'GTC Cuts at: 1318 2866 3676 1318 Size: 1548 810 5956 Fragments arranged by size: 5956 1548 810 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2233 3346 4034 Size: 416 53 83 399 24 816 1113 688 Cuts at: 4034 6045 7003 442 Size: 2011 958 1753 Fragments arranged by size: 2011 1753 1113 958 816 688 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3242 1056 Size: 2186 6128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2969 2984 3062 3152 3317 4037 4520 4694 Size: 1730 15 78 90 165 720 483 174 Cuts at: 4694 4760 4769 4793 6974 1239 Size: 66 9 24 2181 2579 Fragments arranged by size: 2579 2181 1730 720 483 174 165 90 78 66 24 15 9 Bpu10I CC'TnA_GC Cuts at: 2112 2255 2364 2498 4617 4682 2112 Size: 143 109 134 2119 65 5744 Fragments arranged by size: 5744 2119 143 134 109 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 4937 6439 7304 7602 2957 Size: 1980 1502 865 298 3669 Fragments arranged by size: 3669 1980 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3578 4121 4956 6977 882 Size: 617 2079 543 835 2021 2219 Fragments arranged by size: 2219 2079 2021 835 617 543 BsaAI yAC'GTr Cuts at: 493 1204 4261 4938 5266 6105 493 Size: 711 3057 677 328 839 2702 Fragments arranged by size: 3057 2702 839 711 677 328 BsaBI GATnn'nnATC Cuts at: 8308 8308 Size: 8314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4469 5436 6121 6503 Size: 53 83 186 936 2936 967 685 382 Cuts at: 6503 7138 275 Size: 635 1451 Fragments arranged by size: 2936 1451 967 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3630 4358 6747 7145 7419 7566 7977 3630 Size: 728 2389 398 274 147 411 3967 Fragments arranged by size: 3967 2389 728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2396 2426 3785 3815 3836 Size: 30 165 30 993 30 1359 30 21 Cuts at: 3836 3866 4586 4616 4700 4730 4953 4983 5540 Size: 30 720 30 84 30 223 30 557 Cuts at: 5540 5570 1178 Size: 30 3922 Fragments arranged by size: 3922 1359 993 720 557 223 165 84 30 30 30 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2437 2733 4094 4721 4778 1186 Size: 1251 296 1361 627 57 4722 Fragments arranged by size: 4722 1361 1251 627 296 57 BseYI C'CCAG_C Cuts at: 2059 2065 2095 2117 2128 2184 2797 3616 4048 Size: 6 30 22 11 56 613 819 432 Cuts at: 4048 4095 4533 4610 4794 4841 4906 5018 5196 Size: 47 438 77 184 47 65 112 178 Cuts at: 5196 7517 8013 2059 Size: 2321 496 2360 Fragments arranged by size: 2360 2321 819 613 496 438 432 184 178 112 77 65 56 47 47 30 22 11 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2269 2598 3209 3939 4901 5207 5288 Size: 573 533 329 611 730 962 306 81 Cuts at: 5288 5405 1163 Size: 117 4072 Fragments arranged by size: 4072 962 730 611 573 533 329 306 117 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2857 3592 3812 5764 6525 7553 Size: 275 186 856 735 220 1952 761 1028 Cuts at: 7553 1540 Size: 2301 Fragments arranged by size: 2301 1952 1028 856 761 735 275 220 186 BsiHKAI G_wGCw'C Cuts at: 2174 2823 2955 3083 4266 4565 5307 6375 6460 Size: 649 132 128 1183 299 742 1068 85 Cuts at: 6460 7531 2174 Size: 1071 2957 Fragments arranged by size: 2957 1183 1071 1068 742 649 299 132 128 85 BsmI GAATG_Cn' Cuts at: 2826 5825 5918 2826 Size: 2999 93 5222 Fragments arranged by size: 5222 2999 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3689 3689 Size: 8314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2118 2128 2174 2202 2283 2309 Size: 399 306 22 10 46 28 81 26 Cuts at: 2309 2823 2901 2946 2955 3083 3354 3467 3539 Size: 514 78 45 9 128 271 113 72 Cuts at: 3539 3598 3928 3935 3960 4102 4266 4482 4565 Size: 59 330 7 25 142 164 216 83 Cuts at: 4565 4656 4913 4974 4993 5307 5490 5574 5633 Size: 91 257 61 19 314 183 84 59 Cuts at: 5633 6375 6460 7531 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 399 330 314 306 271 257 216 183 164 142 128 113 91 85 84 83 81 78 72 61 59 59 46 45 28 26 25 22 19 10 9 7 BspEI T'CCGG_A Cuts at: 3630 7977 3630 Size: 4347 3967 BspHI T'CATG_A Cuts at: 3285 6203 7933 3285 Size: 2918 1730 3666 Fragments arranged by size: 3666 2918 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2575 2598 4317 5669 844 Size: 1731 23 1719 1352 3489 Fragments arranged by size: 3489 1731 1719 1352 23 BsrBI CCG'CTC Cuts at: 1394 1823 2427 4036 4296 5086 5326 6201 1394 Size: 429 604 1609 260 790 240 875 3507 Fragments arranged by size: 3507 1609 875 790 604 429 260 240 BsrDI GCAATG_nn' Cuts at: 65 1248 5055 6809 6983 65 Size: 1183 3807 1754 174 1396 Fragments arranged by size: 3807 1754 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2337 2766 3467 3587 4209 4419 4465 Size: 110 527 429 701 120 622 210 46 Cuts at: 4465 5429 6958 7145 1700 Size: 964 1529 187 2869 Fragments arranged by size: 2869 1529 964 701 622 527 429 210 187 120 110 46 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8314 BssSI C'ACGA_G Cuts at: 2211 2441 3094 3692 3996 4327 6374 7386 2211 Size: 230 653 598 304 331 2047 1012 3139 Fragments arranged by size: 3139 2047 1012 653 598 331 304 230 BstAPI GCAn_nnn'nTGC Cuts at: 794 3855 4243 5311 5383 794 Size: 3061 388 1068 72 3725 Fragments arranged by size: 3725 3061 1068 388 72 BstBI TT'CG_AA Cuts at: 7118 7118 Size: 8314 BstEII G'GTnAC_C Cuts at: 1184 3712 1184 Size: 2528 5786 BstXI CCAn_nnnn'nTGG Cuts at: 2036 3441 4342 4738 5134 2036 Size: 1405 901 396 396 5216 Fragments arranged by size: 5216 1405 901 396 396 BstYI r'GATC_y Cuts at: 1972 2565 3516 3736 3834 4690 4860 5644 6011 Size: 593 951 220 98 856 170 784 367 Cuts at: 6011 6396 6413 7865 7951 7963 8309 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 951 856 784 593 385 367 346 220 170 98 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3938 5279 3938 Size: 1341 6973 BtgI C'CryG_G Cuts at: 513 1065 2004 2321 3273 4002 4482 5358 513 Size: 552 939 317 952 729 480 876 3469 Fragments arranged by size: 3469 952 939 876 729 552 480 317 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5364 487 Size: 683 56 4138 3437 Fragments arranged by size: 4138 3437 683 56 BtsI GCAGTG_nn' Cuts at: 3015 3207 4781 5574 5830 6624 6644 3015 Size: 192 1574 793 256 794 20 4685 Fragments arranged by size: 4685 1574 794 793 256 192 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8279 DraI TTT'AAA Cuts at: 3493 5618 3493 Size: 2125 6189 DraIII CAC_nnn'GTG Cuts at: 5311 5311 Size: 8314 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3175 7321 809 Size: 507 1859 4146 1802 Fragments arranged by size: 4146 1859 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2682 2713 3216 Size: 54 1425 50 275 18 852 31 503 Cuts at: 3216 3501 3589 3809 4072 5215 5761 6650 8279 Size: 285 88 220 263 1143 546 889 1629 Cuts at: 8279 8 Size: 43 Fragments arranged by size: 1629 1425 1143 889 852 546 503 285 275 263 220 88 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 3809 5761 1537 Size: 275 1777 220 1952 4090 Fragments arranged by size: 4090 1952 1777 275 220 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2915 3632 3884 4644 6244 1353 Size: 659 903 717 252 760 1600 3423 Fragments arranged by size: 3423 1600 903 760 717 659 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3511 3901 4285 6901 7275 7421 Size: 1064 138 1922 390 384 2616 374 146 Cuts at: 7421 387 Size: 1280 Fragments arranged by size: 2616 1922 1280 1064 390 384 374 146 138 EcoICRI GAG'CTC Cuts at: 2172 2821 2172 Size: 649 7665 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2969 2984 3046 3062 3152 3317 4037 Size: 393 1337 15 62 16 90 165 720 Cuts at: 4037 4159 4520 4694 4760 4769 4793 6377 6974 Size: 122 361 174 66 9 24 1584 597 Cuts at: 6974 7761 8029 1239 Size: 787 268 1524 Fragments arranged by size: 1584 1524 1337 787 720 597 393 361 268 174 165 122 90 66 62 24 16 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 2715 4433 4685 2715 Size: 1718 252 6344 Fragments arranged by size: 6344 1718 252 EcoO109I rG'GnC_Cy Cuts at: 2092 2125 2198 2280 2794 2887 3037 3652 3924 Size: 33 73 82 514 93 150 615 272 Cuts at: 3924 3925 4231 4459 4543 4678 4910 2092 Size: 1 306 228 84 135 232 5496 Fragments arranged by size: 5496 615 514 306 272 232 228 150 135 93 84 82 73 33 1 EcoRI G'AATT_C Cuts at: 5623 6001 5623 Size: 378 7936 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3505 3856 3505 Size: 351 7963 FseI GG_CCGG'CC Cuts at: 2341 2341 Size: 8314 FspI TGC'GCA Cuts at: 5498 6820 5498 Size: 1322 6992 HaeII r_GCGC'y Cuts at: 1678 2153 2262 2707 4201 4221 4442 4472 4772 Size: 475 109 445 1494 20 221 30 300 Cuts at: 4772 7461 1678 Size: 2689 2531 Fragments arranged by size: 2689 2531 1494 475 445 300 221 109 30 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2594 2626 3665 3697 4586 Size: 32 135 32 1022 32 1039 32 889 Cuts at: 4586 4618 6955 6987 7029 7061 1373 Size: 32 2337 32 42 32 2626 Fragments arranged by size: 2626 2337 1039 1022 889 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3400 5658 5904 669 Size: 988 281 1462 2258 246 3079 Fragments arranged by size: 3079 2258 1462 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8314 HpaI GTT'AAC Cuts at: 5904 5904 Size: 8314 KasI G'GCGC_C Cuts at: 4468 4468 Size: 8314 KpnI G_GTAC'C Cuts at: 4210 5639 4210 Size: 1429 6885 MfeI C'AATT_G Cuts at: 5913 5913 Size: 8314 MluI A'CGCG_T Cuts at: 8190 8190 Size: 8314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3039 7428 7612 1305 Size: 1734 4389 184 2007 Fragments arranged by size: 4389 2007 1734 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3503 10 Size: 54 1425 343 1671 4821 Fragments arranged by size: 4821 1671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 3254 3542 4343 4401 5036 5126 Size: 648 1524 564 288 801 58 635 90 Cuts at: 5126 5444 6272 6631 6790 518 Size: 318 828 359 159 2042 Fragments arranged by size: 2042 1524 828 801 648 635 564 359 318 288 159 90 58 MspA1I CmG'CkG Cuts at: 1606 2371 2449 2553 2746 3616 3767 4048 4498 Size: 765 78 104 193 870 151 281 450 Cuts at: 4498 4725 4798 5730 6407 7555 7800 1606 Size: 227 73 932 677 1148 245 2120 Fragments arranged by size: 2120 1148 932 870 765 677 450 281 245 227 193 151 104 78 73 NaeI GCC'GGC Cuts at: 2339 4467 5431 2339 Size: 2128 964 5222 Fragments arranged by size: 5222 2128 964 NarI GG'CG_CC Cuts at: 4469 4469 Size: 8314 NcoI C'CATG_G Cuts at: 513 1065 2004 5358 513 Size: 552 939 3354 3469 Fragments arranged by size: 3469 3354 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8314 NgoMIV G'CCGG_C Cuts at: 2337 4465 5429 2337 Size: 2128 964 5222 Fragments arranged by size: 5222 2128 964 NheI G'CTAG_C Cuts at: 1052 3238 1052 Size: 2186 6128 NotI GC'GGCC_GC Cuts at: 5761 5761 Size: 8314 NspI r_CATG'y Cuts at: 1132 1229 3022 5338 5672 7217 1132 Size: 97 1793 2316 334 1545 2229 Fragments arranged by size: 2316 2229 1793 1545 334 97 PasI CC'CwG_GG Cuts at: 1348 2980 3928 4656 5574 1348 Size: 1632 948 728 918 4088 Fragments arranged by size: 4088 1632 948 918 728 PciI A'CATG_T Cuts at: 5334 7213 5334 Size: 1879 6435 PflMI CCAn_nnn'nTGG Cuts at: 1108 5051 1108 Size: 3943 4371 PfoI T'CCnGG_A Cuts at: 3129 4590 3129 Size: 1461 6853 PmeI GTTT'AAAC Cuts at: 5618 5618 Size: 8314 PmlI CAC'GTG Cuts at: 4261 4938 4261 Size: 677 7637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2501 2533 6383 6415 7896 7928 2501 Size: 32 3850 32 1481 32 2887 Fragments arranged by size: 3850 2887 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 3652 4459 4543 2794 Size: 858 807 84 6565 Fragments arranged by size: 6565 858 807 84 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8314 PsiI TTA'TAA Cuts at: 3831 5884 3831 Size: 2053 6261 PspOMI G'GGCC_C Cuts at: 2092 2124 2198 2279 3594 3924 4098 4909 2092 Size: 32 74 81 1315 330 174 811 5497 Fragments arranged by size: 5497 1315 811 330 174 81 74 32 PspXI vC'TCGA_Gb Cuts at: 5321 5321 Size: 8314 PstI C_TGCA'G Cuts at: 830 2586 2609 2886 3334 4047 4239 4380 5118 Size: 1756 23 277 448 713 192 141 738 Cuts at: 5118 5379 5666 830 Size: 261 287 3478 Fragments arranged by size: 3478 1756 738 713 448 287 277 261 192 141 23 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8314 PvuII CAG'CTG Cuts at: 2371 2746 3616 3767 4048 4498 4798 2371 Size: 375 870 151 281 450 300 5887 Fragments arranged by size: 5887 870 450 375 300 281 151 SacI G_AGCT'C Cuts at: 2174 2823 2174 Size: 649 7665 SalI G'TCGA_C Cuts at: 1936 5656 1936 Size: 3720 4594 SanDI GG'GwC_CC Cuts at: 2794 4543 2794 Size: 1749 6565 SapI GCTCTTCn'nnn_ Cuts at: 2012 4644 2012 Size: 2632 5682 SbfI CC_TGCA'GG Cuts at: 5666 5666 Size: 8314 ScaI AGT'ACT Cuts at: 2405 6562 8274 2405 Size: 4157 1712 2445 Fragments arranged by size: 4157 2445 1712 SfcI C'TryA_G Cuts at: 826 1045 2330 2407 2582 2605 2882 3330 4043 Size: 219 1285 77 175 23 277 448 713 Cuts at: 4043 4235 4376 4872 5114 5375 5662 6797 7478 Size: 192 141 496 242 261 287 1135 681 Cuts at: 7478 7669 826 Size: 191 1471 Fragments arranged by size: 1471 1285 1135 713 681 496 448 287 277 261 242 219 192 191 175 141 77 23 SfoI GGC'GCC Cuts at: 4470 4470 Size: 8314 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 SgrAI Cr'CCGG_yG Cuts at: 1700 4465 1700 Size: 2765 5549 SmaI CCC'GGG Cuts at: 1872 2387 2600 3650 3968 5240 5641 1872 Size: 515 213 1050 318 1272 401 4545 Fragments arranged by size: 4545 1272 1050 515 401 318 213 SmlI C'TyrA_G Cuts at: 820 1017 1943 2936 4952 5321 6418 7319 7581 Size: 197 926 993 2016 369 1097 901 262 Cuts at: 7581 820 Size: 1553 Fragments arranged by size: 2016 1553 1097 993 926 901 369 262 197 SnaBI TAC'GTA Cuts at: 493 6105 493 Size: 5612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8314 SphI G_CATG'C Cuts at: 1132 5672 1132 Size: 4540 3774 SspI AAT'ATT Cuts at: 5 52 6238 5 Size: 47 6186 2081 Fragments arranged by size: 6186 2081 47 StuI AGG'CCT Cuts at: 1517 2534 4085 1517 Size: 1017 1551 5746 Fragments arranged by size: 5746 1551 1017 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 3459 4152 4641 5358 5753 Size: 552 939 67 1388 693 489 717 395 Cuts at: 5753 513 Size: 3074 Fragments arranged by size: 3074 1388 939 717 693 552 489 395 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4525 4624 4808 4855 4862 4866 5516 Size: 1255 2945 99 184 47 7 4 650 Cuts at: 5516 5543 6341 6358 6511 6696 7141 325 Size: 27 798 17 153 185 445 1498 Fragments arranged by size: 2945 1498 1255 798 650 445 185 184 153 99 47 27 17 7 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 6560 8272 Size: 274 80 33 51 1443 426 4157 1712 Cuts at: 8272 96 Size: 138 Fragments arranged by size: 4157 1712 1443 426 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3091 3123 3436 3468 4700 4732 4953 4985 8274 Size: 32 313 32 1232 32 221 32 3289 Cuts at: 8274 8306 3091 Size: 32 3099 Fragments arranged by size: 3289 3099 1232 313 221 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8314 XbaI T'CTAG_A Cuts at: 5650 5650 Size: 8314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 4480 3022 Size: 1458 6856 XhoI C'TCGA_G Cuts at: 1943 5321 1943 Size: 3378 4936 XmaI C'CCGG_G Cuts at: 1870 2385 2598 3648 3966 5238 5639 1870 Size: 515 213 1050 318 1272 401 4545 Fragments arranged by size: 4545 1272 1050 515 401 318 213 XmnI GAAnn'nnTTC Cuts at: 4277 4718 6443 4277 Size: 441 1725 6148 Fragments arranged by size: 6148 1725 441 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5437 6122 276 Size: 53 83 186 936 3903 685 2468 Fragments arranged by size: 3903 2468 936 685 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FspAI NruI NsiI PacI PsrI RsrII SacII SexAI SfiI SrfI SwaI