(Linear) (Six-base) MAPSORT of: KIEE5812.seq Check: 1710 from: 1 to: 3609 KIEE5812.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 570 3609 Size: 570 3039 AatII G_ACGT'C Cuts at: 0 3434 3609 Size: 3434 175 Acc65I G'GTAC_C Cuts at: 0 2201 3609 Size: 2201 1408 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1041 2154 3609 Size: 1041 1113 1455 Fragments arranged by size: 1455 1113 1041 AfeI AGC'GCT Cuts at: 0 700 3609 Size: 700 2909 AflIII A'CryG_T Cuts at: 0 3329 3609 Size: 3329 280 AhdI GACnn_n'nnGTC Cuts at: 0 603 1674 3609 Size: 603 1071 1935 Fragments arranged by size: 1935 1071 603 AleI CACnn'nnGTG Cuts at: 0 1249 2396 3609 Size: 1249 1147 1213 Fragments arranged by size: 1249 1213 1147 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3124 3609 Size: 3124 485 AlwNI CAG_nnn'CTG Cuts at: 0 369 1064 1611 1940 2238 2667 3046 3609 Size: 369 695 547 329 298 429 379 563 Fragments arranged by size: 695 563 547 429 379 369 329 298 ApaI G_GGCC'C Cuts at: 0 91 123 197 278 1593 1923 2097 2908 Size: 91 32 74 81 1315 330 174 811 Cuts at: 2908 3609 Size: 701 Fragments arranged by size: 1315 811 701 330 174 91 81 74 32 ApaLI G'TGCA_C Cuts at: 0 2257 2556 3298 3609 Size: 2257 299 742 311 Fragments arranged by size: 2257 742 311 299 AvaI C'yCGr_G Cuts at: 0 162 380 593 919 1356 1643 1961 2159 Size: 162 218 213 326 437 287 318 198 Cuts at: 2159 3233 3316 3609 Size: 1074 83 293 Fragments arranged by size: 1074 437 326 318 293 287 218 213 198 162 83 BamHI G'GATC_C Cuts at: 0 560 1731 2685 3609 Size: 560 1171 954 924 Fragments arranged by size: 1171 954 924 560 BanI G'GyrC_C Cuts at: 0 481 936 1459 1927 2201 2463 2832 3609 Size: 481 455 523 468 274 262 369 777 Fragments arranged by size: 777 523 481 468 455 369 274 262 BanII G_rGCy'C Cuts at: 0 91 113 123 169 197 278 304 818 Size: 91 22 10 46 28 81 26 514 Cuts at: 818 896 1593 1923 1955 2097 2477 2651 2908 Size: 78 697 330 32 142 380 174 257 Cuts at: 2908 2988 3485 3609 Size: 80 497 124 Fragments arranged by size: 697 514 497 380 330 257 174 142 124 91 81 80 78 46 32 28 26 22 10 BbeI G_GCGC'C Cuts at: 0 2467 3609 Size: 2467 1142 BbsI GAAGACnn'nnnn_ Cuts at: 0 1486 3476 3609 Size: 1486 1990 133 Fragments arranged by size: 1990 1486 133 BbvCI CC'TCA_GC Cuts at: 0 250 2612 2677 3609 Size: 250 2362 65 932 Fragments arranged by size: 2362 932 250 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1065 1099 3609 Size: 1065 34 2510 Fragments arranged by size: 2510 1065 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1149 3609 Size: 1149 2460 BglI GCCn_nnn'nGGC Cuts at: 0 100 469 1000 1270 2110 2415 2513 3609 Size: 100 369 531 270 840 305 98 1096 Fragments arranged by size: 1096 840 531 369 305 270 100 98 BglII A'GATC_T Cuts at: 0 1511 3609 Size: 1511 2098 BlpI GC'TnA_GC Cuts at: 0 521 2209 2440 2629 2938 3609 Size: 521 1688 231 189 309 671 Fragments arranged by size: 1688 671 521 309 231 189 Bme1580I G_kGCm'C Cuts at: 0 91 123 197 278 941 1349 1462 1534 Size: 91 32 74 81 663 408 113 72 Cuts at: 1534 1593 1923 1930 2097 2261 2560 2908 2969 Size: 59 330 7 167 164 299 348 61 Cuts at: 2969 3302 3569 3609 Size: 333 267 40 Fragments arranged by size: 663 408 348 333 330 299 267 167 164 113 91 81 74 72 61 59 40 32 7 BmgBI CAC'GTC Cuts at: 0 861 1671 3609 Size: 861 810 1938 Fragments arranged by size: 1938 861 810 BmrI ACTGGGnnnn_n' Cuts at: 0 228 1341 2029 3609 Size: 228 1113 688 1580 Fragments arranged by size: 1580 1113 688 228 BmtI G_CTAG'C Cuts at: 0 1237 3609 Size: 1237 2372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 979 1057 1147 1312 2032 2515 2689 Size: 964 15 78 90 165 720 483 174 Cuts at: 2689 2755 2764 2788 3609 Size: 66 9 24 821 Fragments arranged by size: 964 821 720 483 174 165 90 78 66 24 15 9 Bpu10I CC'TnA_GC Cuts at: 0 107 250 359 493 2612 2677 3609 Size: 107 143 109 134 2119 65 932 Fragments arranged by size: 2119 932 143 134 109 107 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 952 2932 3609 Size: 952 1980 677 Fragments arranged by size: 1980 952 677 BsaI GGTCTCn'nnnn_ Cuts at: 0 1573 2116 2951 3609 Size: 1573 543 835 658 Fragments arranged by size: 1573 835 658 543 BsaAI yAC'GTr Cuts at: 0 2256 2933 3261 3609 Size: 2256 677 328 348 Fragments arranged by size: 2256 677 348 328 BsaHI Gr'CG_yC Cuts at: 0 2464 3431 3609 Size: 2464 967 178 Fragments arranged by size: 2464 967 178 BsaWI w'CCGG_w Cuts at: 0 1625 2353 3609 Size: 1625 728 1256 Fragments arranged by size: 1625 1256 728 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 391 421 1780 1810 1831 1861 2581 2611 Size: 391 30 1359 30 21 30 720 30 Cuts at: 2611 2695 2725 2948 2978 3535 3565 3609 Size: 84 30 223 30 557 30 44 Fragments arranged by size: 1359 720 557 391 223 84 44 30 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 432 728 2089 2716 2773 3609 Size: 432 296 1361 627 57 836 Fragments arranged by size: 1361 836 627 432 296 57 BseYI C'CCAG_C Cuts at: 0 54 60 90 112 123 179 792 1611 Size: 54 6 30 22 11 56 613 819 Cuts at: 1611 2043 2090 2528 2605 2789 2836 2901 3013 Size: 432 47 438 77 184 47 65 112 Cuts at: 3013 3191 3609 Size: 178 418 Fragments arranged by size: 819 613 438 432 418 184 178 112 77 65 56 54 47 47 30 22 11 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 264 593 1204 1934 2896 3202 3283 3400 Size: 264 329 611 730 962 306 81 117 Cuts at: 3400 3609 Size: 209 Fragments arranged by size: 962 730 611 329 306 264 209 117 81 BsiEI CG_ry'CG Cuts at: 0 852 1587 1807 3609 Size: 852 735 220 1802 Fragments arranged by size: 1802 852 735 220 BsiHKAI G_wGCw'C Cuts at: 0 169 818 950 1078 2261 2560 3302 3609 Size: 169 649 132 128 1183 299 742 307 Fragments arranged by size: 1183 742 649 307 299 169 132 128 BsmI GAATG_Cn' Cuts at: 0 821 3609 Size: 821 2788 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1684 3609 Size: 1684 1925 Bsp1286I G_dGCh'C Cuts at: 0 91 113 123 169 197 278 304 818 Size: 91 22 10 46 28 81 26 514 Cuts at: 818 896 941 950 1078 1349 1462 1534 1593 Size: 78 45 9 128 271 113 72 59 Cuts at: 1593 1923 1930 1955 2097 2261 2477 2560 2651 Size: 330 7 25 142 164 216 83 91 Cuts at: 2651 2908 2969 2988 3302 3485 3569 3609 Size: 257 61 19 314 183 84 40 Fragments arranged by size: 514 330 314 271 257 216 183 164 142 128 113 91 91 84 83 81 78 72 61 59 46 45 40 28 26 25 22 19 10 9 7 BspEI T'CCGG_A Cuts at: 0 1625 3609 Size: 1625 1984 BspHI T'CATG_A Cuts at: 0 1280 3609 Size: 1280 2329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 570 593 2312 3609 Size: 570 23 1719 1297 Fragments arranged by size: 1719 1297 570 23 BsrBI CCG'CTC Cuts at: 0 422 2031 2291 3081 3321 3609 Size: 422 1609 260 790 240 288 Fragments arranged by size: 1609 790 422 288 260 240 BsrDI GCAATG_nn' Cuts at: 0 3050 3609 Size: 3050 559 BsrFI r'CCGG_y Cuts at: 0 332 761 1462 1582 2204 2414 2460 3424 Size: 332 429 701 120 622 210 46 964 Cuts at: 3424 3609 Size: 185 Fragments arranged by size: 964 701 622 429 332 210 185 120 46 BssSI C'ACGA_G Cuts at: 0 206 436 1089 1687 1991 2322 3609 Size: 206 230 653 598 304 331 1287 Fragments arranged by size: 1287 653 598 331 304 230 206 BstAPI GCAn_nnn'nTGC Cuts at: 0 1850 2238 3306 3378 3609 Size: 1850 388 1068 72 231 Fragments arranged by size: 1850 1068 388 231 72 BstEII G'GTnAC_C Cuts at: 0 1707 3609 Size: 1707 1902 BstXI CCAn_nnnn'nTGG Cuts at: 0 31 1436 2337 2733 3129 3609 Size: 31 1405 901 396 396 480 Fragments arranged by size: 1405 901 480 396 396 31 BstYI r'GATC_y Cuts at: 0 560 1511 1731 1829 2685 2855 3609 Size: 560 951 220 98 856 170 754 Fragments arranged by size: 951 856 754 560 220 170 98 Bsu36I CC'TnA_GG Cuts at: 0 1933 3274 3609 Size: 1933 1341 335 Fragments arranged by size: 1933 1341 335 BtgI C'CryG_G Cuts at: 0 316 1268 1997 2477 3353 3609 Size: 316 952 729 480 876 256 Fragments arranged by size: 952 876 729 480 316 256 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3359 3609 Size: 3359 250 BtsI GCAGTG_nn' Cuts at: 0 1010 1202 2776 3569 3609 Size: 1010 192 1574 793 40 Fragments arranged by size: 1574 1010 793 192 40 DraI TTT'AAA Cuts at: 0 1488 3609 Size: 1488 2121 DraIII CAC_nnn'GTG Cuts at: 0 3306 3609 Size: 3306 303 DrdI GACnn_nn'nnGTC Cuts at: 0 1170 3609 Size: 1170 2439 EaeI y'GGCC_r Cuts at: 0 677 708 1211 1496 1584 1804 2067 3210 Size: 677 31 503 285 88 220 263 1143 Cuts at: 3210 3609 Size: 399 Fragments arranged by size: 1143 677 503 399 285 263 220 88 31 EagI C'GGCC_G Cuts at: 0 1584 1804 3609 Size: 1584 220 1805 Fragments arranged by size: 1805 1584 220 EarI CTCTTCn'nnn_ Cuts at: 0 7 910 1627 1879 2639 3609 Size: 7 903 717 252 760 970 Fragments arranged by size: 970 903 760 717 252 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1506 1896 2280 3609 Size: 1506 390 384 1329 Fragments arranged by size: 1506 1329 390 384 EcoICRI GAG'CTC Cuts at: 0 167 816 3609 Size: 167 649 2793 Fragments arranged by size: 2793 649 167 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 979 1041 1057 1147 1312 2032 2154 Size: 964 15 62 16 90 165 720 122 Cuts at: 2154 2515 2689 2755 2764 2788 3609 Size: 361 174 66 9 24 821 Fragments arranged by size: 964 821 720 361 174 165 122 90 66 62 24 16 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 710 2428 2680 3609 Size: 710 1718 252 929 Fragments arranged by size: 1718 929 710 252 EcoO109I rG'GnC_Cy Cuts at: 0 87 120 193 275 789 882 1032 1647 Size: 87 33 73 82 514 93 150 615 Cuts at: 1647 1919 1920 2226 2454 2538 2673 2905 3609 Size: 272 1 306 228 84 135 232 704 Fragments arranged by size: 704 615 514 306 272 232 228 150 135 93 87 84 82 73 33 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1500 1851 3609 Size: 1500 351 1758 Fragments arranged by size: 1758 1500 351 FseI GG_CCGG'CC Cuts at: 0 336 3609 Size: 336 3273 FspI TGC'GCA Cuts at: 0 3493 3609 Size: 3493 116 HaeII r_GCGC'y Cuts at: 0 148 257 702 2196 2216 2437 2467 2767 Size: 148 109 445 1494 20 221 30 300 Cuts at: 2767 3609 Size: 842 Fragments arranged by size: 1494 842 445 300 221 148 109 30 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 589 621 1660 1692 2581 2613 3609 Size: 589 32 1039 32 889 32 996 Fragments arranged by size: 1039 996 889 589 32 32 32 HincII GTy'rAC Cuts at: 0 1395 3609 Size: 1395 2214 KasI G'GCGC_C Cuts at: 0 2463 3609 Size: 2463 1146 KpnI G_GTAC'C Cuts at: 0 2205 3609 Size: 2205 1404 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1034 3609 Size: 1034 2575 MscI TGG'CCA Cuts at: 0 1498 3609 Size: 1498 2111 MslI CAynn'nnrTG Cuts at: 0 685 1249 1537 2338 2396 3031 3121 3439 Size: 685 564 288 801 58 635 90 318 Cuts at: 3439 3609 Size: 170 Fragments arranged by size: 801 685 635 564 318 288 170 90 58 MspA1I CmG'CkG Cuts at: 0 366 444 548 741 1611 1762 2043 2493 Size: 366 78 104 193 870 151 281 450 Cuts at: 2493 2720 2793 3609 Size: 227 73 816 Fragments arranged by size: 870 816 450 366 281 227 193 151 104 78 73 NaeI GCC'GGC Cuts at: 0 334 2462 3426 3609 Size: 334 2128 964 183 Fragments arranged by size: 2128 964 334 183 NarI GG'CG_CC Cuts at: 0 2464 3609 Size: 2464 1145 NcoI C'CATG_G Cuts at: 0 3353 3609 Size: 3353 256 NgoMIV G'CCGG_C Cuts at: 0 332 2460 3424 3609 Size: 332 2128 964 185 Fragments arranged by size: 2128 964 332 185 NheI G'CTAG_C Cuts at: 0 1233 3609 Size: 1233 2376 NspI r_CATG'y Cuts at: 0 1017 3333 3609 Size: 1017 2316 276 Fragments arranged by size: 2316 1017 276 PasI CC'CwG_GG Cuts at: 0 975 1923 2651 3569 3609 Size: 975 948 728 918 40 Fragments arranged by size: 975 948 918 728 40 PciI A'CATG_T Cuts at: 0 3329 3609 Size: 3329 280 PflMI CCAn_nnn'nTGG Cuts at: 0 3046 3609 Size: 3046 563 PfoI T'CCnGG_A Cuts at: 0 1124 2585 3609 Size: 1124 1461 1024 Fragments arranged by size: 1461 1124 1024 PmlI CAC'GTG Cuts at: 0 2256 2933 3609 Size: 2256 677 676 Fragments arranged by size: 2256 677 676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 496 528 3609 Size: 496 32 3081 Fragments arranged by size: 3081 496 32 PpuMI rG'GwC_Cy Cuts at: 0 789 1647 2454 2538 3609 Size: 789 858 807 84 1071 Fragments arranged by size: 1071 858 807 789 84 PsiI TTA'TAA Cuts at: 0 1826 3609 Size: 1826 1783 PspOMI G'GGCC_C Cuts at: 0 87 119 193 274 1589 1919 2093 2904 Size: 87 32 74 81 1315 330 174 811 Cuts at: 2904 3609 Size: 705 Fragments arranged by size: 1315 811 705 330 174 87 81 74 32 PspXI vC'TCGA_Gb Cuts at: 0 3316 3609 Size: 3316 293 PstI C_TGCA'G Cuts at: 0 581 604 881 1329 2042 2234 2375 3113 Size: 581 23 277 448 713 192 141 738 Cuts at: 3113 3374 3609 Size: 261 235 Fragments arranged by size: 738 713 581 448 277 261 235 192 141 23 PvuII CAG'CTG Cuts at: 0 366 741 1611 1762 2043 2493 2793 3609 Size: 366 375 870 151 281 450 300 816 Fragments arranged by size: 870 816 450 375 366 300 281 151 SacI G_AGCT'C Cuts at: 0 169 818 3609 Size: 169 649 2791 Fragments arranged by size: 2791 649 169 SanDI GG'GwC_CC Cuts at: 0 789 2538 3609 Size: 789 1749 1071 Fragments arranged by size: 1749 1071 789 SapI GCTCTTCn'nnn_ Cuts at: 0 7 2639 3609 Size: 7 2632 970 Fragments arranged by size: 2632 970 7 ScaI AGT'ACT Cuts at: 0 400 3609 Size: 400 3209 SfcI C'TryA_G Cuts at: 0 325 402 577 600 877 1325 2038 2230 Size: 325 77 175 23 277 448 713 192 Cuts at: 2230 2371 2867 3109 3370 3609 Size: 141 496 242 261 239 Fragments arranged by size: 713 496 448 325 277 261 242 239 192 175 141 77 23 SfoI GGC'GCC Cuts at: 0 2465 3609 Size: 2465 1144 SgrAI Cr'CCGG_yG Cuts at: 0 2460 3609 Size: 2460 1149 SmaI CCC'GGG Cuts at: 0 382 595 1645 1963 3235 3609 Size: 382 213 1050 318 1272 374 Fragments arranged by size: 1272 1050 382 374 318 213 SmlI C'TyrA_G Cuts at: 0 931 2947 3316 3609 Size: 931 2016 369 293 Fragments arranged by size: 2016 931 369 293 StuI AGG'CCT Cuts at: 0 529 2080 3609 Size: 529 1551 1529 Fragments arranged by size: 1551 1529 529 StyI C'CwwG_G Cuts at: 0 66 1454 2147 2636 3353 3609 Size: 66 1388 693 489 717 256 Fragments arranged by size: 1388 717 693 489 256 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2520 2619 2803 2850 2857 2861 3511 3538 Size: 2520 99 184 47 7 4 650 27 Cuts at: 3538 3609 Size: 71 Fragments arranged by size: 2520 650 184 99 71 47 27 7 4 TatI w'GTAC_w Cuts at: 0 398 3609 Size: 398 3211 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1086 1118 1431 1463 2695 2727 2948 2980 Size: 1086 32 313 32 1232 32 221 32 Cuts at: 2980 3609 Size: 629 Fragments arranged by size: 1232 1086 629 313 221 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1017 2475 3609 Size: 1017 1458 1134 Fragments arranged by size: 1458 1134 1017 XhoI C'TCGA_G Cuts at: 0 3316 3609 Size: 3316 293 XmaI C'CCGG_G Cuts at: 0 380 593 1643 1961 3233 3609 Size: 380 213 1050 318 1272 376 Fragments arranged by size: 1272 1050 380 376 318 213 XmnI GAAnn'nnTTC Cuts at: 0 2272 2713 3609 Size: 2272 441 896 Fragments arranged by size: 2272 896 441 ZraI GAC'GTC Cuts at: 0 3432 3609 Size: 3432 177 Enzymes that do cut: AarI AatII Acc65I AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SanDI SapI ScaI SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI AclI AflII AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI BclI BfrBI BplI BsaBI BsiWI BsrGI BssHII BstBI BstZ17I ClaI CspCI EcoRI EcoRV FspAI HindIII HpaI MfeI MluI NdeI NotI NruI NsiI PacI PmeI PshAI PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI