(Circular) (Six-base) MAPSORT of: pFN21AE2379.seq Check: 6872 from: 1 to: 8182 pFN21AE2379 8182 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2207 2207 Size: 8182 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4386 5992 278 Size: 53 83 186 936 2850 1606 2468 Fragments arranged by size: 2850 2468 1606 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5525 1937 Size: 3588 4594 Acc65I G'GTAC_C Cuts at: 5503 5503 Size: 8182 AclI AA'CG_TT Cuts at: 1563 6309 6682 1563 Size: 4746 373 3063 Fragments arranged by size: 4746 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 3323 3596 3844 4175 4630 6245 7629 Size: 887 804 273 248 331 455 1615 1384 Cuts at: 7629 7897 1632 Size: 268 1917 Fragments arranged by size: 1917 1615 1384 887 804 455 331 273 268 248 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7985 AflIII A'CryG_T Cuts at: 1203 3978 4533 7081 8058 1203 Size: 2775 555 2548 977 1327 Fragments arranged by size: 2775 2548 1327 977 555 AgeI A'CCGG_T Cuts at: 7013 7013 Size: 8182 AhdI GACnn_n'nnGTC Cuts at: 6911 6911 Size: 8182 AleI CACnn'nnGTG Cuts at: 1166 3875 4310 1166 Size: 2709 435 5038 Fragments arranged by size: 5038 2709 435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2963 1184 Size: 1779 6403 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3374 3406 3374 Size: 32 8150 AlwNI CAG_nnn'CTG Cuts at: 2260 3136 3358 4000 4036 5004 5452 5925 7497 Size: 876 222 642 36 968 448 473 1572 Cuts at: 7497 2260 Size: 2945 Fragments arranged by size: 2945 1572 968 876 642 473 448 222 36 ApaI G_GGCC'C Cuts at: 2453 2685 4264 4477 4841 5377 5437 2453 Size: 232 1579 213 364 536 60 5198 Fragments arranged by size: 5198 1579 536 364 232 213 60 ApaLI G'TGCA_C Cuts at: 3667 6239 7395 3667 Size: 2572 1156 4454 Fragments arranged by size: 4454 2572 1156 ApoI r'AATT_y Cuts at: 1493 5491 5717 5869 7978 1493 Size: 3998 226 152 2109 1697 Fragments arranged by size: 3998 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6736 160 Size: 872 5704 1606 Fragments arranged by size: 5704 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8182 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2529 2575 2678 2757 2785 2820 Size: 73 163 423 46 103 79 28 35 Cuts at: 2820 4006 4094 4353 4609 5092 5106 5254 5368 Size: 1186 88 259 256 483 14 148 114 Cuts at: 5368 5473 5507 1870 Size: 105 34 4545 Fragments arranged by size: 4545 1186 483 423 259 256 163 148 114 105 103 88 79 73 46 35 34 28 14 BamHI G'GATC_C Cuts at: 5512 5879 5512 Size: 367 7815 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2267 2341 2570 2614 3146 Size: 325 217 627 480 74 229 44 532 Cuts at: 3146 3178 4870 4970 5503 6958 618 Size: 32 1692 100 533 1455 1842 Fragments arranged by size: 1842 1692 1455 627 533 532 480 325 229 217 100 74 44 32 BanII G_rGCy'C Cuts at: 1391 2453 2679 2685 2792 4264 4361 4477 4616 Size: 1062 226 6 107 1472 97 116 139 Cuts at: 4616 4796 4841 4920 5040 5377 5437 5501 1391 Size: 180 45 79 120 337 60 64 4072 Fragments arranged by size: 4072 1472 1062 337 226 180 139 120 116 107 97 79 64 60 45 6 BbeI G_GCGC'C Cuts at: 2271 2574 3150 4974 2271 Size: 303 576 1824 5479 Fragments arranged by size: 5479 1824 576 303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3265 3584 928 Size: 971 1366 319 5526 Fragments arranged by size: 5526 1366 971 319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2811 2845 6373 6407 1903 Size: 34 50 34 790 34 3528 34 3678 Fragments arranged by size: 3678 3528 790 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6074 7284 6074 Size: 1210 6972 BclI T'GATC_A Cuts at: 1548 1548 Size: 8182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2579 2855 3462 Size: 107 122 71 631 605 907 276 607 Cuts at: 3462 3813 4777 6793 136 Size: 351 964 2016 1525 Fragments arranged by size: 2016 1525 964 907 631 607 605 351 276 122 107 71 BglII A'GATC_T Cuts at: 3918 8177 3918 Size: 4259 3923 BlpI GC'TnA_GC Cuts at: 5599 5599 Size: 8182 Bme1580I G_kGCm'C Cuts at: 1790 2346 2453 2685 3671 4013 4264 4477 4841 Size: 556 107 232 986 342 251 213 364 Cuts at: 4841 4875 5377 5437 6243 7399 1790 Size: 34 502 60 806 1156 2573 Fragments arranged by size: 2573 1156 986 806 556 502 364 342 251 232 213 107 60 34 BmgBI CAC'GTC Cuts at: 1318 2026 2596 3154 5116 1318 Size: 708 570 558 1962 4384 Fragments arranged by size: 4384 1962 708 570 558 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5335 5913 6871 Size: 416 53 83 399 24 3918 578 958 Cuts at: 6871 442 Size: 1753 Fragments arranged by size: 3918 1753 958 578 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3698 3966 1056 Size: 2642 268 5272 Fragments arranged by size: 5272 2642 268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2776 4541 4592 4795 4837 4864 5071 5123 Size: 1537 1765 51 203 42 27 207 52 Cuts at: 5123 5180 6842 1239 Size: 57 1662 2579 Fragments arranged by size: 2579 1765 1662 1537 207 203 57 52 51 42 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 6307 7172 7470 3017 Size: 3290 865 298 3729 Fragments arranged by size: 3729 3290 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 6845 882 Size: 617 3657 1689 2219 Fragments arranged by size: 3657 2219 1689 617 BsaAI yAC'GTr Cuts at: 493 1204 2668 5973 493 Size: 711 1464 3305 2702 Fragments arranged by size: 3305 2702 1464 711 BsaBI GATnn'nnATC Cuts at: 8176 8176 Size: 8182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2268 2571 2606 2781 Size: 53 83 186 936 735 303 35 175 Cuts at: 2781 3147 4383 4971 5407 5989 6371 7006 275 Size: 366 1236 588 436 582 382 635 1451 Fragments arranged by size: 1451 1236 936 735 635 588 582 436 382 366 303 186 175 83 53 35 BsaWI w'CCGG_w Cuts at: 2301 6615 7013 7287 7434 7845 2301 Size: 4314 398 274 147 411 2638 Fragments arranged by size: 4314 2638 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4334 4364 4781 4811 1178 Size: 30 165 30 2931 30 417 30 4549 Fragments arranged by size: 4549 2931 417 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2705 3458 3572 4112 5060 5372 1186 Size: 1102 417 753 114 540 948 312 3996 Fragments arranged by size: 3996 1102 948 753 540 417 312 114 BseYI C'CCAG_C Cuts at: 2263 2618 4319 5063 5428 5445 7385 7881 2263 Size: 355 1701 744 365 17 1940 496 2564 Fragments arranged by size: 2564 1940 1701 744 496 365 355 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2179 2195 3746 4178 5196 5438 1163 Size: 573 443 16 1551 432 1018 242 3907 Fragments arranged by size: 3907 1551 1018 573 443 432 242 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2549 2859 3466 3648 4772 5632 Size: 275 186 548 310 607 182 1124 860 Cuts at: 5632 6393 7421 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1124 1028 860 761 607 548 310 275 186 182 BsiHKAI G_wGCw'C Cuts at: 3671 5040 6243 6328 7399 3671 Size: 1369 1203 85 1071 4454 Fragments arranged by size: 4454 1369 1203 1071 85 BsmI GAATG_Cn' Cuts at: 5693 5786 5693 Size: 93 8089 BsmBI CGTCTCn'nnnn_ Cuts at: 3302 3898 3302 Size: 596 7586 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2453 2679 2685 2792 3671 4013 Size: 399 556 107 226 6 107 879 342 Cuts at: 4013 4264 4361 4477 4616 4796 4841 4875 4920 Size: 251 97 116 139 180 45 34 45 Cuts at: 4920 5040 5377 5437 5501 6243 6328 7399 1391 Size: 120 337 60 64 742 85 1071 2174 Fragments arranged by size: 2174 1071 879 742 556 399 342 337 251 226 180 139 120 116 107 107 97 85 64 60 45 45 34 6 BspEI T'CCGG_A Cuts at: 7845 7845 Size: 8182 BspHI T'CATG_A Cuts at: 2431 6071 7801 2431 Size: 3640 1730 2812 Fragments arranged by size: 3640 2812 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 3540 4335 4524 4596 5537 844 Size: 1363 1333 795 189 72 941 3489 Fragments arranged by size: 3489 1363 1333 941 795 189 72 BsrBI CCG'CTC Cuts at: 1394 1823 2695 4206 6069 1394 Size: 429 872 1511 1863 3507 Fragments arranged by size: 3507 1863 1511 872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6677 6851 65 Size: 1183 5429 174 1396 Fragments arranged by size: 5429 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 4767 6826 7013 1700 Size: 110 198 2759 2059 187 2869 Fragments arranged by size: 2869 2759 2059 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8182 BssHII G'CGCG_C Cuts at: 1925 2733 2878 1925 Size: 808 145 7229 Fragments arranged by size: 7229 808 145 BssSI C'ACGA_G Cuts at: 2975 6242 7254 2975 Size: 3267 1012 3903 Fragments arranged by size: 3903 3267 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2260 2935 794 Size: 1466 675 6041 Fragments arranged by size: 6041 1466 675 BstBI TT'CG_AA Cuts at: 6986 6986 Size: 8182 BstEII G'GTnAC_C Cuts at: 1184 3124 1184 Size: 1940 6242 BstXI CCAn_nnnn'nTGG Cuts at: 2467 2467 Size: 8182 BstYI r'GATC_y Cuts at: 1972 3918 4068 4368 4500 5512 5879 6264 6281 Size: 1946 150 300 132 1012 367 385 17 Cuts at: 6281 7733 7819 7831 8177 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1946 1452 1012 385 367 346 300 150 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2583 2610 2655 2685 4781 513 Size: 552 1518 27 45 30 2096 3914 Fragments arranged by size: 3914 2096 1518 552 45 30 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2080 2736 3454 3667 487 Size: 683 56 854 656 718 213 5002 Fragments arranged by size: 5002 854 718 683 656 213 56 BtsI GCAGTG_nn' Cuts at: 2258 2933 4208 4219 4831 5485 5698 6492 6512 Size: 675 1275 11 612 654 213 794 20 Cuts at: 6512 2258 Size: 3928 Fragments arranged by size: 3928 1275 794 675 654 612 213 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8147 DraI TTT'AAA Cuts at: 5486 5486 Size: 8182 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7189 809 Size: 507 5873 1802 Fragments arranged by size: 5873 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 2856 3463 Size: 54 1425 50 275 18 910 116 607 Cuts at: 3463 3513 3645 3984 4266 4392 4769 5184 5629 Size: 50 132 339 282 126 377 415 445 Cuts at: 5629 6518 8147 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 607 445 415 377 339 282 275 132 126 116 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2856 3463 3645 4769 5629 1537 Size: 275 1044 607 182 1124 860 4090 Fragments arranged by size: 4090 1124 1044 860 607 275 182 EarI CTCTTCn'nnn_ Cuts at: 1353 4934 6112 1353 Size: 3581 1178 3423 Fragments arranged by size: 3581 3423 1178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2483 2712 4853 6769 7143 7289 Size: 1064 138 894 229 2141 1916 374 146 Cuts at: 7289 387 Size: 1280 Fragments arranged by size: 2141 1916 1280 1064 894 374 229 146 138 EcoICRI GAG'CTC Cuts at: 5038 5038 Size: 8182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2776 3323 3596 3844 4175 4541 Size: 393 887 257 547 273 248 331 366 Cuts at: 4541 4592 4630 4795 4837 4864 5071 5123 5180 Size: 51 38 165 42 27 207 52 57 Cuts at: 5180 6245 6842 7629 7897 1239 Size: 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 887 787 597 547 393 366 331 273 268 257 248 207 165 57 52 51 42 38 27 EcoNI CCTnn'n_nnAGG Cuts at: 3302 4520 4678 4925 3302 Size: 1218 158 247 6559 Fragments arranged by size: 6559 1218 247 158 EcoO109I rG'GnC_Cy Cuts at: 2421 2449 2450 2711 3133 4261 4324 4474 4837 Size: 28 1 261 422 1128 63 150 363 Cuts at: 4837 4890 5258 5373 5374 5396 5433 5434 2421 Size: 53 368 115 1 22 37 1 5169 Fragments arranged by size: 5169 1128 422 368 363 261 150 115 63 53 37 28 22 1 1 1 EcoRI G'AATT_C Cuts at: 5491 5869 5491 Size: 378 7804 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4690 4690 Size: 8182 FseI GG_CCGG'CC Cuts at: 4771 4771 Size: 8182 FspI TGC'GCA Cuts at: 2939 6688 2939 Size: 3749 4433 HaeII r_GCGC'y Cuts at: 1678 2233 2271 2522 2574 3150 3848 4093 4974 Size: 555 38 251 52 576 698 245 881 Cuts at: 4974 5146 7329 1678 Size: 172 2183 2531 Fragments arranged by size: 2531 2183 881 698 576 555 251 245 172 52 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3614 3646 4060 4092 4781 Size: 32 135 32 2042 32 414 32 689 Cuts at: 4781 4813 6823 6855 6897 6929 1373 Size: 32 2010 32 42 32 2626 Fragments arranged by size: 2626 2042 2010 689 414 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5526 5772 669 Size: 988 281 3588 246 3079 Fragments arranged by size: 3588 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8182 HpaI GTT'AAC Cuts at: 5772 5772 Size: 8182 KasI G'GCGC_C Cuts at: 2267 2570 3146 4970 2267 Size: 303 576 1824 5479 Fragments arranged by size: 5479 1824 576 303 KpnI G_GTAC'C Cuts at: 5507 5507 Size: 8182 MfeI C'AATT_G Cuts at: 5781 5781 Size: 8182 MluI A'CGCG_T Cuts at: 8058 8058 Size: 8182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2994 4575 5013 7296 7480 1305 Size: 1689 1581 438 2283 184 2007 Fragments arranged by size: 2283 2007 1689 1581 438 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4268 4394 5186 10 Size: 54 1425 343 2436 126 792 3006 Fragments arranged by size: 3006 2436 1425 792 343 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3506 3875 4031 4139 4310 6140 Size: 648 843 1497 369 156 108 171 1830 Cuts at: 6140 6499 6658 518 Size: 359 159 2042 Fragments arranged by size: 2042 1830 1497 843 648 369 359 171 159 156 108 MspA1I CmG'CkG Cuts at: 1606 2463 2612 2687 2796 2943 3195 3358 3804 Size: 857 149 75 109 147 252 163 446 Cuts at: 3804 3997 5200 5284 5428 5598 6275 7423 7668 Size: 193 1203 84 144 170 677 1148 245 Cuts at: 7668 1606 Size: 2120 Fragments arranged by size: 2120 1203 1148 857 677 446 252 245 193 170 163 149 147 144 109 84 75 NaeI GCC'GGC Cuts at: 4769 4769 Size: 8182 NarI GG'CG_CC Cuts at: 2268 2571 3147 4971 2268 Size: 303 576 1824 5479 Fragments arranged by size: 5479 1824 576 303 NcoI C'CATG_G Cuts at: 513 1065 2655 513 Size: 552 1590 6040 Fragments arranged by size: 6040 1590 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8182 NgoMIV G'CCGG_C Cuts at: 4767 4767 Size: 8182 NheI G'CTAG_C Cuts at: 1052 3694 3962 1052 Size: 2642 268 5272 Fragments arranged by size: 5272 2642 268 NotI GC'GGCC_GC Cuts at: 5629 5629 Size: 8182 NspI r_CATG'y Cuts at: 1132 1229 4292 4319 4537 5540 7085 1132 Size: 97 3063 27 218 1003 1545 2229 Fragments arranged by size: 3063 2229 1545 1003 218 97 27 PasI CC'CwG_GG Cuts at: 1348 3142 1348 Size: 1794 6388 PciI A'CATG_T Cuts at: 4533 7081 4533 Size: 2548 5634 PflMI CCAn_nnn'nTGG Cuts at: 1108 2463 3358 5347 1108 Size: 1355 895 1989 3943 Fragments arranged by size: 3943 1989 1355 895 PfoI T'CCnGG_A Cuts at: 2046 2046 Size: 8182 PmeI GTTT'AAAC Cuts at: 5486 5486 Size: 8182 PmlI CAC'GTG Cuts at: 2668 2668 Size: 8182 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6251 6283 7764 7796 6251 Size: 32 1481 32 6637 Fragments arranged by size: 6637 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2421 2711 3133 4324 2421 Size: 290 422 1191 6279 Fragments arranged by size: 6279 1191 422 290 PshAI GACnn'nnGTC Cuts at: 1613 5222 1613 Size: 3609 4573 PsiI TTA'TAA Cuts at: 5752 5752 Size: 8182 PspOMI G'GGCC_C Cuts at: 2449 2681 4260 4473 4837 5373 5433 2449 Size: 232 1579 213 364 536 60 5198 Fragments arranged by size: 5198 1579 536 364 232 213 60 PspXI vC'TCGA_Gb Cuts at: 4094 4094 Size: 8182 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4529 4561 4529 Size: 32 8150 PstI C_TGCA'G Cuts at: 830 2801 3177 4179 4218 5199 5534 830 Size: 1971 376 1002 39 981 335 3478 Fragments arranged by size: 3478 1971 1002 981 376 335 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8182 PvuII CAG'CTG Cuts at: 2463 2796 5200 5428 2463 Size: 333 2404 228 5217 Fragments arranged by size: 5217 2404 333 228 SacI G_AGCT'C Cuts at: 5040 5040 Size: 8182 SacII CC_GC'GG Cuts at: 2613 2688 2613 Size: 75 8107 SalI G'TCGA_C Cuts at: 1936 5524 1936 Size: 3588 4594 SanDI GG'GwC_CC Cuts at: 2421 2711 2421 Size: 290 7892 SapI GCTCTTCn'nnn_ Cuts at: 4934 4934 Size: 8182 SbfI CC_TGCA'GG Cuts at: 4179 5534 4179 Size: 1355 6827 ScaI AGT'ACT Cuts at: 4103 6430 8142 4103 Size: 2327 1712 4143 Fragments arranged by size: 4143 2327 1712 SfcI C'TryA_G Cuts at: 826 1045 2797 3173 4175 4214 5195 5204 5236 Size: 219 1752 376 1002 39 981 9 32 Cuts at: 5236 5530 6665 7346 7537 826 Size: 294 1135 681 191 1471 Fragments arranged by size: 1752 1471 1135 1002 981 681 376 294 219 191 39 32 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3813 4777 3813 Size: 964 7218 SfoI GGC'GCC Cuts at: 2269 2572 3148 4972 2269 Size: 303 576 1824 5479 Fragments arranged by size: 5479 1824 576 303 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8182 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8182 SmaI CCC'GGG Cuts at: 1872 2531 2577 2759 4355 4611 5094 5108 5256 Size: 659 46 182 1596 256 483 14 148 Cuts at: 5256 5370 5475 5509 1872 Size: 114 105 34 4545 Fragments arranged by size: 4545 1596 659 483 256 182 148 114 105 46 34 14 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 4094 6286 7187 7449 820 Size: 197 926 1089 1062 2192 901 262 1553 Fragments arranged by size: 2192 1553 1089 1062 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5973 493 Size: 5480 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8182 SphI G_CATG'C Cuts at: 1132 4319 5540 1132 Size: 3187 1221 3774 Fragments arranged by size: 3774 3187 1221 SspI AAT'ATT Cuts at: 5 52 6106 5 Size: 47 6054 2081 Fragments arranged by size: 6054 2081 47 StuI AGG'CCT Cuts at: 1517 4447 1517 Size: 2930 5252 StyI C'CwwG_G Cuts at: 513 1065 2655 4245 4725 5316 5621 513 Size: 552 1590 1590 480 591 305 3074 Fragments arranged by size: 3074 1590 1590 591 552 480 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2632 2946 3143 6209 6226 6379 6564 Size: 1255 1052 314 197 3066 17 153 185 Cuts at: 6564 7009 325 Size: 445 1498 Fragments arranged by size: 3066 1498 1255 1052 445 314 197 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3165 4002 4101 Size: 274 80 33 51 1443 1188 837 99 Cuts at: 4101 4341 6428 8140 96 Size: 240 2087 1712 138 Fragments arranged by size: 2087 1712 1443 1188 837 274 240 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3171 3203 4103 4135 4547 4579 8142 8174 3171 Size: 32 900 32 412 32 3563 32 3179 Fragments arranged by size: 3563 3179 900 412 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4385 1316 Size: 3069 5113 XbaI T'CTAG_A Cuts at: 5518 5518 Size: 8182 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2107 2464 2800 3445 3721 5314 2107 Size: 357 336 645 276 1593 4975 Fragments arranged by size: 4975 1593 645 357 336 276 XhoI C'TCGA_G Cuts at: 1943 4094 1943 Size: 2151 6031 XmaI C'CCGG_G Cuts at: 1870 2529 2575 2757 4353 4609 5092 5106 5254 Size: 659 46 182 1596 256 483 14 148 Cuts at: 5254 5368 5473 5507 1870 Size: 114 105 34 4545 Fragments arranged by size: 4545 1596 659 483 256 182 148 114 105 46 34 14 XmnI GAAnn'nnTTC Cuts at: 2042 3920 4160 6311 2042 Size: 1878 240 2151 3913 Fragments arranged by size: 3913 2151 1878 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4384 5990 276 Size: 53 83 186 936 2850 1606 2468 Fragments arranged by size: 2850 2468 1606 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI PacI RsrII SexAI SrfI SwaI