(Circular) (Six-base) MAPSORT of: pFN21AE6043.seq Check: 6813 from: 1 to: 5656 pFN21AE6043 5656 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2795 3719 5103 5371 1632 Size: 1163 924 1384 268 1917 Fragments arranged by size: 1917 1384 1163 924 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2721 2723 4555 5532 1203 Size: 1518 2 1832 977 1327 Fragments arranged by size: 1832 1518 1327 977 2 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 2724 1166 Size: 1558 4098 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2807 2839 2807 Size: 32 5624 AlwNI CAG_nnn'CTG Cuts at: 2064 2248 3399 4971 2064 Size: 184 1151 1572 2749 Fragments arranged by size: 2749 1572 1151 184 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2036 2924 2965 3191 3343 5452 1493 Size: 543 888 41 226 152 2109 1697 Fragments arranged by size: 2109 1697 888 543 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2981 1870 Size: 73 130 908 4545 Fragments arranged by size: 4545 908 130 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2977 4432 618 Size: 325 217 627 1190 1455 1842 Fragments arranged by size: 1842 1455 1190 627 325 217 BanII G_rGCy'C Cuts at: 1391 2975 1391 Size: 1584 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BbvCI CC'TCA_GC Cuts at: 2241 2241 Size: 5656 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 2148 2527 1548 Size: 600 379 4677 Fragments arranged by size: 4677 600 379 BfrBI ATG'CAT Cuts at: 2413 2413 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 3717 4873 1790 Size: 1927 1156 2573 Fragments arranged by size: 2573 1927 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2074 2296 3387 Size: 416 53 83 399 24 657 222 1091 Cuts at: 3387 4345 442 Size: 958 1753 Fragments arranged by size: 1753 1091 958 657 416 399 222 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2779 4316 1239 Size: 1540 1537 2579 Fragments arranged by size: 2579 1540 1537 Bpu10I CC'TnA_GC Cuts at: 2241 2241 Size: 5656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2083 4319 882 Size: 617 584 2236 2219 Fragments arranged by size: 2236 2219 617 584 BsaAI yAC'GTr Cuts at: 493 1204 2724 3447 493 Size: 711 1520 723 2702 Fragments arranged by size: 2702 1520 723 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 2807 2837 1178 Size: 30 165 30 1164 30 210 30 3997 Fragments arranged by size: 3997 1164 210 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2036 2572 1186 Size: 850 536 4270 Fragments arranged by size: 4270 850 536 BseYI C'CCAG_C Cuts at: 2122 4859 5355 2122 Size: 2737 496 2423 Fragments arranged by size: 2737 2423 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 2498 2530 1163 Size: 573 580 182 32 4289 Fragments arranged by size: 4289 580 573 182 32 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3717 3802 4873 3717 Size: 85 1071 4500 Fragments arranged by size: 4500 1071 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2975 3717 3802 4873 1391 Size: 399 1185 742 85 1071 2174 Fragments arranged by size: 2174 1185 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2113 2593 1184 Size: 929 480 4247 Fragments arranged by size: 4247 929 480 BstYI r'GATC_y Cuts at: 1972 2685 2986 3353 3738 3755 5207 5293 5305 Size: 713 301 367 385 17 1452 86 12 Cuts at: 5305 5651 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 301 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2879 2879 Size: 5656 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 3172 3966 3986 3172 Size: 794 20 4842 Fragments arranged by size: 4842 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2075 4663 809 Size: 507 759 2588 1802 Fragments arranged by size: 2588 1802 759 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 2436 2766 Size: 54 1425 50 275 18 573 33 330 Cuts at: 2766 3103 3992 5621 8 Size: 337 889 1629 43 Fragments arranged by size: 1629 1425 889 573 337 330 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3586 1353 Size: 2233 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2779 2795 3719 4316 5103 5371 1239 Size: 393 1147 16 924 597 787 268 1524 Fragments arranged by size: 1524 1147 924 787 597 393 268 16 EcoO109I rG'GnC_Cy Cuts at: 2901 2901 Size: 5656 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2519 2551 2567 2599 4297 Size: 32 135 32 947 32 16 32 1698 Cuts at: 4297 4329 4371 4403 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1698 947 135 42 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2937 3000 3246 669 Size: 988 281 999 63 246 3079 Fragments arranged by size: 3079 999 988 281 246 63 HindIII A'AGCT_T Cuts at: 748 2712 748 Size: 1964 3692 HpaI GTT'AAC Cuts at: 2937 3246 2937 Size: 309 5347 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2438 2768 10 Size: 54 1425 343 606 330 2898 Fragments arranged by size: 2898 1425 606 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2177 2600 2603 2724 3614 3973 Size: 648 942 69 423 3 121 890 359 Cuts at: 3973 4132 518 Size: 159 2042 Fragments arranged by size: 2042 942 890 648 423 359 159 121 69 3 MspA1I CmG'CkG Cuts at: 1606 2245 2500 3072 3749 4897 5142 1606 Size: 639 255 572 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 639 572 255 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NsiI A_TGCA'T Cuts at: 2415 2415 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 1108 Size: 956 4700 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PmlI CAC'GTG Cuts at: 2724 2724 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2807 2839 3725 3757 5238 5270 2807 Size: 32 886 32 1481 32 3193 Fragments arranged by size: 3193 1481 886 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2245 2500 2245 Size: 255 5401 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 3004 4139 4820 5011 826 Size: 219 1959 1135 681 191 1471 Fragments arranged by size: 1959 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2004 2322 3095 513 Size: 552 939 318 773 3074 Fragments arranged by size: 3074 939 773 552 318 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2017 2315 3683 3700 3853 4038 4483 Size: 1255 437 298 1368 17 153 185 445 Cuts at: 4483 325 Size: 1498 Fragments arranged by size: 1498 1368 1255 445 437 298 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2040 2714 2822 3785 2040 Size: 674 108 963 3911 Fragments arranged by size: 3911 963 674 108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI