################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiWI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MroI MseI MspA1I MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1144 GTCGAC GT^[AC][GT]AC AccII 26 CGCG CG^CG ApaI 880 GGGCCC GGGCC^C Asp700I 839 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 658 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 554 GGCACC G^G[CT][GA]CC BsaBI 957 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 427 GAGGAG GAGGAG(10/8) BsiEI 999 CGGCCG CG[GA][CT]^CG BsmBI 1255 GAGACG CGTCTC(1/5) BspHI 314 TCATGA T^CATGA BstAPI 854 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 26 CGCG CG^CG BstXI 559 CCAGCCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 999 CGGCCG C^GGCCG EclXI 999 CGGCCG C^GGCCG Eco52I 999 CGGCCG C^GGCCG EcoRI 581 GAATTC G^AATTC HaeII 1266 GGCGCT [GA]GCGC^[CT] HgaI 24 GACGC GACGC(5/10) HincII 1144 GTCGAC GT[CT]^[GA]AC HindII 1144 GTCGAC GT[CT]^[GA]AC HindIII 278 AAGCTT A^AGCTT MaeII 1258 ACGT A^CGT MamI 957 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 26 CGCG CG^CG PflFI 658 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 314 TCATGA T^CATGA SalI 1144 GTCGAC G^TCGAC SexAI 119 ACCAGGT A^CC[AT]GGT SmaI 590 CCCGGG CCC^GGG StuI 1064 AGGCCT AGG^CCT Tsp45I 709 GTCAC ^GT[GC]AC Tth111I 658 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 590 CCCGGG C^CCGGG XmaI 590 CCCGGG C^CCGGG XmnI 839 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 331 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 813 CAGGAACTG AvaI 166 CTCGGG C^[CT]CG[GA]G 590 CCCGGG BalI 137 TGGCCA TGG^CCA 1127 TGGCCA BbsI 350 GAAGAC GAAGAC(2/6) 67 GTCTTC BpuAI 350 GAAGAC GAAGAC(2/6) 67 GTCTTC BsmI 681 GAATGC GAATGC(1/-1) 465 GCATTC BsoBI 166 CTCGGG C^[CT]CG[GA]G 590 CCCGGG BstYI 171 GGATCT [GA]^GATC[CT] 804 GGATCT Cac8I 374 GCCGGC GC[ACGT]^[ACGT]GC 1224 GCCGGC DraII 179 AGGGCCT [GA]G^G[ACGT]CC[CT] 880 GGGCCCT EcoO109I 179 AGGGCCT [GA]G^G[ACGT]CC[CT] 880 GGGCCCT FauI 371 CCCGC CCCGC(4/6) 20 GCGGG MaeIII 404 GTTAC ^GT[ACGT]AC 709 GTCAC MflI 171 GGATCT [GA]^GATC[CT] 804 GGATCT MluNI 137 TGGCCA TGG^CCA 1127 TGGCCA MscI 137 TGGCCA TGG^CCA 1127 TGGCCA MslI 327 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 795 CATCGGCGTG NaeI 374 GCCGGC GCC^GGC 1224 GCCGGC NgoMIV 374 GCCGGC G^CCGGC 1224 GCCGGC PflMI 192 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 417 CCACACCCTGG PleI 131 GAGTC GAGTC(4/5) 289 GAGTC SacI 703 GAGCTC GAGCT^C 1189 GAGCTC TfiI 785 GAATC G^A[AT]TC 1296 GAATC Van91I 192 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 417 CCACACCCTGG XhoII 171 GGATCT [GA]^GATC[CT] 804 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp509I TspRI TthHB8I XcmI