(Circular) (Six-base) MAPSORT of: pFN21AE5537.seq Check: 1574 from: 1 to: 6013 pFN21AE5537 6013 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3150 3356 1937 Size: 1213 206 4594 Fragments arranged by size: 4594 1213 206 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2059 2389 2555 3169 4076 5460 5728 1632 Size: 427 330 166 614 907 1384 268 1917 Fragments arranged by size: 1917 1384 907 614 427 330 268 166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 2341 2823 3756 5328 2341 Size: 482 933 1572 3026 Fragments arranged by size: 3026 1572 933 482 ApaI G_GGCC'C Cuts at: 2889 2889 Size: 6013 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 2110 2490 2586 3322 3548 3700 5809 1493 Size: 617 380 96 736 226 152 2109 1697 Fragments arranged by size: 2109 1697 736 617 380 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2595 3338 1870 Size: 73 228 424 743 4545 Fragments arranged by size: 4545 743 424 228 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2559 3334 4789 618 Size: 325 217 627 772 775 1455 1842 Fragments arranged by size: 1842 1455 775 772 627 325 217 BanII G_rGCy'C Cuts at: 1391 2712 2889 3021 3198 3332 1391 Size: 1321 177 132 177 134 4072 Fragments arranged by size: 4072 1321 177 177 134 132 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2065 2362 928 Size: 971 166 297 4579 Fragments arranged by size: 4579 971 297 166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4204 4238 1903 Size: 34 50 34 2183 34 3678 Fragments arranged by size: 3678 2183 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2015 2889 4074 5230 1790 Size: 225 874 1185 1156 2573 Fragments arranged by size: 2573 1185 1156 874 225 BmgBI CAC'GTC Cuts at: 1318 3264 1318 Size: 1946 4067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3163 3216 3744 Size: 416 53 83 399 24 1746 53 528 Cuts at: 3744 4702 442 Size: 958 1753 Fragments arranged by size: 1753 1746 958 528 416 399 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2201 2312 2405 2450 2568 3035 4673 Size: 801 161 111 93 45 118 467 1638 Cuts at: 4673 1239 Size: 2579 Fragments arranged by size: 2579 1638 801 467 161 118 111 93 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 5003 5301 4138 Size: 865 298 4850 Fragments arranged by size: 4850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4676 882 Size: 617 3177 2219 Fragments arranged by size: 3177 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 2966 6007 2966 Size: 3041 2972 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3820 4202 4837 275 Size: 53 83 186 936 2287 382 635 1451 Fragments arranged by size: 2287 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2873 2903 3059 3089 1178 Size: 30 165 30 1470 30 156 30 4102 Fragments arranged by size: 4102 1470 165 156 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 1186 Size: 1261 4752 BseYI C'CCAG_C Cuts at: 2221 2231 2563 5216 5712 2221 Size: 10 332 2653 496 2522 Fragments arranged by size: 2653 2522 496 332 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3007 3463 4224 5252 1540 Size: 275 186 1006 456 761 1028 2301 Fragments arranged by size: 2301 1028 1006 761 456 275 186 BsiHKAI G_wGCw'C Cuts at: 2712 3198 3294 4074 4159 5230 2712 Size: 486 96 780 85 1071 3495 Fragments arranged by size: 3495 1071 780 486 96 85 BsmI GAATG_Cn' Cuts at: 2470 2692 3524 3617 2470 Size: 222 832 93 4866 Fragments arranged by size: 4866 832 222 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3254 3254 Size: 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2712 2889 3021 3198 3294 3332 Size: 399 225 697 177 132 177 96 38 Cuts at: 3332 4074 4159 5230 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 697 399 225 177 177 132 96 85 38 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 2319 3902 5632 2319 Size: 1583 1730 2700 Fragments arranged by size: 2700 1730 1583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3368 844 Size: 2524 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2379 2618 3229 4657 4844 1700 Size: 110 569 239 611 1428 187 2869 Fragments arranged by size: 2869 1428 611 569 239 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2865 794 Size: 2071 3942 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstXI CCAn_nnnn'nTGG Cuts at: 2571 2571 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2176 2809 3343 3710 4095 4112 5564 5650 Size: 204 633 534 367 385 17 1452 86 Cuts at: 5650 5662 6008 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 633 534 385 367 346 204 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2049 2364 2691 2941 3085 3249 513 Size: 552 984 315 327 250 144 164 3277 Fragments arranged by size: 3277 984 552 327 315 250 164 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 3529 4323 4343 3529 Size: 794 20 5199 Fragments arranged by size: 5199 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3004 3132 Size: 54 1425 50 275 18 312 862 128 Cuts at: 3132 3231 3460 4349 5978 8 Size: 99 229 889 1629 43 Fragments arranged by size: 1629 1425 889 862 312 275 229 128 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3004 3460 1537 Size: 275 1192 456 4090 Fragments arranged by size: 4090 1192 456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3943 1353 Size: 2590 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2074 2779 4600 4974 5120 387 Size: 1064 138 485 705 1821 374 146 1280 Fragments arranged by size: 1821 1280 1064 705 485 374 146 138 EcoICRI GAG'CTC Cuts at: 2710 3196 2710 Size: 486 5527 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2059 2201 2312 2389 2405 2450 Size: 393 408 19 142 111 77 16 45 Cuts at: 2450 2555 2568 3035 3169 4076 4673 5460 5728 Size: 105 13 467 134 907 597 787 268 Cuts at: 5728 1239 Size: 1524 Fragments arranged by size: 1524 907 787 597 467 408 393 268 142 134 111 105 77 45 19 16 13 EcoO109I rG'GnC_Cy Cuts at: 2185 2886 2185 Size: 701 5312 EcoRI G'AATT_C Cuts at: 2586 3322 3700 2586 Size: 736 378 4899 Fragments arranged by size: 4899 736 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2224 3199 2224 Size: 975 5038 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 3275 5160 1678 Size: 1597 1885 2531 Fragments arranged by size: 2531 1885 1597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 2775 2807 4654 Size: 32 135 32 896 32 275 32 1847 Cuts at: 4654 4686 4728 4760 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1847 896 275 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3151 3357 3603 669 Size: 988 281 1213 206 246 3079 Fragments arranged by size: 3079 1213 988 281 246 206 HindIII A'AGCT_T Cuts at: 748 2283 748 Size: 1535 4478 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2880 5127 5311 1305 Size: 1575 2247 184 2007 Fragments arranged by size: 2247 2007 1575 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 3134 10 Size: 54 1425 343 312 990 2889 Fragments arranged by size: 2889 1425 990 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 2804 3971 4330 4489 518 Size: 648 1170 468 1167 359 159 2042 Fragments arranged by size: 2042 1170 1167 648 468 359 159 MspA1I CmG'CkG Cuts at: 1606 3429 4106 5254 5499 1606 Size: 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 245 NaeI GCC'GGC Cuts at: 2381 3231 2381 Size: 850 5163 NcoI C'CATG_G Cuts at: 513 1065 2364 2691 2941 3085 513 Size: 552 1299 327 250 144 3441 Fragments arranged by size: 3441 1299 552 327 250 144 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NgoMIV G'CCGG_C Cuts at: 2379 3229 2379 Size: 850 5163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 3371 4916 1132 Size: 97 2142 1545 2229 Fragments arranged by size: 2229 2142 1545 97 PasI CC'CwG_GG Cuts at: 1348 2036 1348 Size: 688 5325 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 2428 1108 Size: 1095 225 4693 Fragments arranged by size: 4693 1095 225 PfoI T'CCnGG_A Cuts at: 2017 3198 2017 Size: 1181 4832 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 5595 5627 4082 Size: 32 1481 32 4468 Fragments arranged by size: 4468 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspOMI G'GGCC_C Cuts at: 2885 2885 Size: 6013 PstI C_TGCA'G Cuts at: 830 3365 830 Size: 2535 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SacI G_AGCT'C Cuts at: 2712 3198 2712 Size: 486 5527 SalI G'TCGA_C Cuts at: 1936 3149 3355 1936 Size: 1213 206 4594 Fragments arranged by size: 4594 1213 206 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SexAI A'CCwGG_T Cuts at: 2124 2124 Size: 6013 SfcI C'TryA_G Cuts at: 826 1045 3361 4496 5177 5368 826 Size: 219 2316 1135 681 191 1471 Fragments arranged by size: 2316 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 2597 3340 1872 Size: 725 743 4545 Fragments arranged by size: 4545 743 725 SmlI C'TyrA_G Cuts at: 820 1017 1943 4117 5018 5280 820 Size: 197 926 2174 901 262 1553 Fragments arranged by size: 2174 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 3071 1517 Size: 1554 4459 StyI C'CwwG_G Cuts at: 513 1065 2364 2691 2941 3054 3085 3452 513 Size: 552 1299 327 250 113 31 367 3074 Fragments arranged by size: 3074 1299 552 367 327 250 113 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2577 2624 4040 4057 4210 4395 4840 Size: 1255 997 47 1416 17 153 185 445 Cuts at: 4840 325 Size: 1498 Fragments arranged by size: 1498 1416 1255 997 445 185 153 47 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3096 4259 5971 Size: 274 80 33 51 1443 1119 1163 1712 Cuts at: 5971 96 Size: 138 Fragments arranged by size: 1712 1443 1163 1119 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2873 2905 3228 3260 5973 6005 2873 Size: 32 323 32 2713 32 2881 Fragments arranged by size: 2881 2713 323 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2666 1316 Size: 1350 4663 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 3061 3127 2239 Size: 822 66 5125 Fragments arranged by size: 5125 822 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 2595 3338 1870 Size: 725 743 4545 Fragments arranged by size: 4545 743 725 XmnI GAAnn'nnTTC Cuts at: 2848 4142 2848 Size: 1294 4719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI