(Linear) (Six-base) MAPSORT of: KIEE5537.seq Check: 9484 from: 1 to: 1308 KIEE5537.orf With 182 enzymes: SgfI * January 29, 2014 12:04 .. AccI GT'mk_AC Cuts at: 0 1145 1308 Size: 1145 163 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 54 384 550 1164 1308 Size: 54 330 166 614 144 Fragments arranged by size: 614 330 166 144 54 AlwNI CAG_nnn'CTG Cuts at: 0 336 818 1308 Size: 336 482 490 Fragments arranged by size: 490 482 336 ApaI G_GGCC'C Cuts at: 0 884 1308 Size: 884 424 ApoI r'AATT_y Cuts at: 0 105 485 581 1308 Size: 105 380 96 727 Fragments arranged by size: 727 380 105 96 AvaI C'yCGr_G Cuts at: 0 166 590 1308 Size: 166 424 718 Fragments arranged by size: 718 424 166 BanI G'GyrC_C Cuts at: 0 554 1308 Size: 554 754 BanII G_rGCy'C Cuts at: 0 707 884 1016 1193 1308 Size: 707 177 132 177 115 Fragments arranged by size: 707 177 177 132 115 BbsI GAAGACnn'nnnn_ Cuts at: 0 60 357 1308 Size: 60 297 951 Fragments arranged by size: 951 297 60 Bme1580I G_kGCm'C Cuts at: 0 10 884 1308 Size: 10 874 424 Fragments arranged by size: 874 424 10 BmgBI CAC'GTC Cuts at: 0 1259 1308 Size: 1259 49 BmrI ACTGGGnnnn_n' Cuts at: 0 1158 1211 1308 Size: 1158 53 97 Fragments arranged by size: 1158 97 53 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 196 307 400 445 563 1030 1308 Size: 35 161 111 93 45 118 467 278 Fragments arranged by size: 467 278 161 118 111 93 45 35 BsaBI GATnn'nnATC Cuts at: 0 961 1308 Size: 961 347 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 868 898 1054 1084 1308 Size: 868 30 156 30 224 Fragments arranged by size: 868 224 156 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 442 1308 Size: 442 866 BseYI C'CCAG_C Cuts at: 0 216 226 558 1308 Size: 216 10 332 750 Fragments arranged by size: 750 332 216 10 BsiEI CG_ry'CG Cuts at: 0 1002 1308 Size: 1002 306 BsiHKAI G_wGCw'C Cuts at: 0 707 1193 1289 1308 Size: 707 486 96 19 Fragments arranged by size: 707 486 96 19 BsmI GAATG_Cn' Cuts at: 0 465 687 1308 Size: 465 222 621 Fragments arranged by size: 621 465 222 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1249 1308 Size: 1249 59 Bsp1286I G_dGCh'C Cuts at: 0 10 707 884 1016 1193 1289 1308 Size: 10 697 177 132 177 96 19 Fragments arranged by size: 697 177 177 132 96 19 10 BspHI T'CATG_A Cuts at: 0 314 1308 Size: 314 994 BsrFI r'CCGG_y Cuts at: 0 374 613 1224 1308 Size: 374 239 611 84 Fragments arranged by size: 611 374 239 84 BstAPI GCAn_nnn'nTGC Cuts at: 0 860 1308 Size: 860 448 BstXI CCAn_nnnn'nTGG Cuts at: 0 566 1308 Size: 566 742 BstYI r'GATC_y Cuts at: 0 171 804 1308 Size: 171 633 504 Fragments arranged by size: 633 504 171 BtgI C'CryG_G Cuts at: 0 44 359 686 936 1080 1244 1308 Size: 44 315 327 250 144 164 64 Fragments arranged by size: 327 315 250 164 144 64 44 EaeI y'GGCC_r Cuts at: 0 137 999 1127 1226 1308 Size: 137 862 128 99 82 Fragments arranged by size: 862 137 128 99 82 EagI C'GGCC_G Cuts at: 0 999 1308 Size: 999 309 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 69 774 1308 Size: 69 705 534 Fragments arranged by size: 705 534 69 EcoICRI GAG'CTC Cuts at: 0 705 1191 1308 Size: 705 486 117 Fragments arranged by size: 705 486 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 35 54 196 307 384 400 445 550 Size: 35 19 142 111 77 16 45 105 Cuts at: 550 563 1030 1164 1308 Size: 13 467 134 144 Fragments arranged by size: 467 144 142 134 111 105 77 45 35 19 16 13 EcoO109I rG'GnC_Cy Cuts at: 0 180 881 1308 Size: 180 701 427 Fragments arranged by size: 701 427 180 EcoRI G'AATT_C Cuts at: 0 581 1308 Size: 581 727 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 219 1194 1308 Size: 219 975 114 Fragments arranged by size: 975 219 114 HaeII r_GCGC'y Cuts at: 0 1270 1308 Size: 1270 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 770 802 1308 Size: 463 32 275 32 506 Fragments arranged by size: 506 463 275 32 32 HincII GTy'rAC Cuts at: 0 1146 1308 Size: 1146 162 HindIII A'AGCT_T Cuts at: 0 278 1308 Size: 278 1030 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 875 1308 Size: 875 433 MscI TGG'CCA Cuts at: 0 139 1129 1308 Size: 139 990 179 Fragments arranged by size: 990 179 139 MslI CAynn'nnrTG Cuts at: 0 331 799 1308 Size: 331 468 509 Fragments arranged by size: 509 468 331 NaeI GCC'GGC Cuts at: 0 376 1226 1308 Size: 376 850 82 Fragments arranged by size: 850 376 82 NcoI C'CATG_G Cuts at: 0 359 686 936 1080 1308 Size: 359 327 250 144 228 Fragments arranged by size: 359 327 250 228 144 NgoMIV G'CCGG_C Cuts at: 0 374 1224 1308 Size: 374 850 84 Fragments arranged by size: 850 374 84 PasI CC'CwG_GG Cuts at: 0 31 1308 Size: 31 1277 PflMI CCAn_nnn'nTGG Cuts at: 0 198 423 1308 Size: 198 225 885 Fragments arranged by size: 885 225 198 PfoI T'CCnGG_A Cuts at: 0 12 1193 1308 Size: 12 1181 115 Fragments arranged by size: 1181 115 12 PspOMI G'GGCC_C Cuts at: 0 880 1308 Size: 880 428 SacI G_AGCT'C Cuts at: 0 707 1193 1308 Size: 707 486 115 Fragments arranged by size: 707 486 115 SalI G'TCGA_C Cuts at: 0 1144 1308 Size: 1144 164 SexAI A'CCwGG_T Cuts at: 0 119 1308 Size: 119 1189 SmaI CCC'GGG Cuts at: 0 592 1308 Size: 592 716 StuI AGG'CCT Cuts at: 0 1066 1308 Size: 1066 242 StyI C'CwwG_G Cuts at: 0 359 686 936 1049 1080 1308 Size: 359 327 250 113 31 228 Fragments arranged by size: 359 327 250 228 113 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 572 619 1308 Size: 572 47 689 Fragments arranged by size: 689 572 47 TatI w'GTAC_w Cuts at: 0 1091 1308 Size: 1091 217 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 868 900 1223 1255 1308 Size: 868 32 323 32 53 Fragments arranged by size: 868 323 53 32 32 Tth111I GACn'n_nGTC Cuts at: 0 661 1308 Size: 661 647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 234 1056 1122 1308 Size: 234 822 66 186 Fragments arranged by size: 822 234 186 66 XmaI C'CCGG_G Cuts at: 0 590 1308 Size: 590 718 XmnI GAAnn'nnTTC Cuts at: 0 843 1308 Size: 843 465 Enzymes that do cut: AccI AcuI AlwNI ApaI ApoI AvaI BanI BanII BbsI Bme1580I BmgBI BmrI BpmI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI BstAPI BstXI BstYI BtgI EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII MmeI MscI MslI NaeI NcoI NgoMIV PasI PflMI PfoI PspOMI SacI SalI SexAI SmaI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsgI BsiWI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoNI EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI MluI MspA1I NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI