(Circular) (Six-base) MAPSORT of: pFN21AE1892.seq Check: 7430 from: 1 to: 5968 pFN21AE1892 5968 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3077 3077 Size: 5968 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2693 3311 1937 Size: 756 618 4594 Fragments arranged by size: 4594 756 618 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 3251 4031 5415 5683 1632 Size: 875 744 780 1384 268 1917 Fragments arranged by size: 1917 1384 875 780 744 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 4867 5844 1203 Size: 3664 977 1327 Fragments arranged by size: 3664 1327 977 AgeI A'CCGG_T Cuts at: 4799 4799 Size: 5968 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5968 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5968 AlwNI CAG_nnn'CTG Cuts at: 2049 2944 3711 5283 2049 Size: 895 767 1572 2734 Fragments arranged by size: 2734 1572 895 767 ApaI G_GGCC'C Cuts at: 2032 2032 Size: 5968 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2063 2137 2902 3131 3277 3503 3655 5764 Size: 570 74 765 229 146 226 152 2109 Cuts at: 5764 1493 Size: 1697 Fragments arranged by size: 2109 1697 765 570 229 226 152 146 74 AseI AT'TA_AT Cuts at: 160 1032 2091 4522 160 Size: 872 1059 2431 1606 Fragments arranged by size: 2431 1606 1059 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 2550 3293 1870 Size: 73 607 743 4545 Fragments arranged by size: 4545 743 607 73 BamHI G'GATC_C Cuts at: 2617 3298 3665 2617 Size: 681 367 4920 Fragments arranged by size: 4920 681 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3289 4744 618 Size: 325 217 627 1502 1455 1842 Fragments arranged by size: 1842 1502 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2032 3287 1391 Size: 641 1255 4072 Fragments arranged by size: 4072 1255 641 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2729 3239 928 Size: 971 830 510 3657 Fragments arranged by size: 3657 971 830 510 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2007 2961 3860 5070 2007 Size: 954 899 1210 2905 Fragments arranged by size: 2905 1210 954 899 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 3385 3385 Size: 5968 Bme1580I G_kGCm'C Cuts at: 1790 2032 3047 4029 5185 1790 Size: 242 1015 982 1156 2573 Fragments arranged by size: 2573 1156 1015 982 242 BmgBI CAC'GTC Cuts at: 1318 3219 1318 Size: 1901 4067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2508 3118 3699 Size: 416 53 83 399 24 1091 610 581 Cuts at: 3699 4657 442 Size: 958 1753 Fragments arranged by size: 1753 1091 958 610 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3093 1056 Size: 2037 3931 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4628 1239 Size: 3389 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4093 4958 5256 4093 Size: 865 298 4805 Fragments arranged by size: 4805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4631 882 Size: 617 3132 2219 Fragments arranged by size: 3132 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3775 4157 4792 275 Size: 53 83 186 936 2242 382 635 1451 Fragments arranged by size: 2242 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4401 4799 5073 5220 5631 4401 Size: 398 274 147 411 4738 Fragments arranged by size: 4738 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2807 2837 1178 Size: 30 165 30 1404 30 4309 Fragments arranged by size: 4309 1404 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2167 2452 3227 1186 Size: 981 285 775 3927 Fragments arranged by size: 3927 981 775 285 BseYI C'CCAG_C Cuts at: 3223 5171 5667 3223 Size: 1948 496 3524 Fragments arranged by size: 3524 1948 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 3055 1163 Size: 573 1173 146 4076 Fragments arranged by size: 4076 1173 573 146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2474 2519 2523 3418 4179 5207 Size: 275 186 473 45 4 895 761 1028 Cuts at: 5207 1540 Size: 2301 Fragments arranged by size: 2301 1028 895 761 473 275 186 45 4 BsiHKAI G_wGCw'C Cuts at: 4029 4114 5185 4029 Size: 85 1071 4812 Fragments arranged by size: 4812 1071 85 BsmI GAATG_Cn' Cuts at: 2539 3137 3479 3572 2539 Size: 598 342 93 4935 Fragments arranged by size: 4935 598 342 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2100 3209 2100 Size: 1109 4859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 3047 3287 4029 4114 5185 1391 Size: 399 242 1015 240 742 85 1071 2174 Fragments arranged by size: 2174 1071 1015 742 399 242 240 85 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2292 2931 3077 3323 844 Size: 1448 639 146 246 3489 Fragments arranged by size: 3489 1448 639 246 146 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2409 4463 4637 65 Size: 1183 1161 2054 174 1396 Fragments arranged by size: 2054 1396 1183 1161 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2469 2518 4612 4799 1700 Size: 110 659 49 2094 187 2869 Fragments arranged by size: 2869 2094 659 187 110 49 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 2396 4028 5040 2396 Size: 1632 1012 3324 Fragments arranged by size: 3324 1632 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5968 BstBI TT'CG_AA Cuts at: 4772 4772 Size: 5968 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5968 BstXI CCAn_nnnn'nTGG Cuts at: 2897 3028 2897 Size: 131 5837 BstYI r'GATC_y Cuts at: 1972 2246 2559 2617 3298 3665 4050 4067 5519 Size: 274 313 58 681 367 385 17 1452 Cuts at: 5519 5605 5617 5963 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 681 385 367 346 313 274 86 58 17 12 BstZ17I GTA'TAC Cuts at: 2694 2694 Size: 5968 BtgI C'CryG_G Cuts at: 513 1065 2836 513 Size: 552 1771 3645 Fragments arranged by size: 3645 1771 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 2274 2571 3204 3484 4278 4298 2274 Size: 297 633 280 794 20 3944 Fragments arranged by size: 3944 794 633 297 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 2233 3272 2233 Size: 1039 4929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4975 809 Size: 507 3659 1802 Fragments arranged by size: 3659 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2471 2516 Size: 54 1425 50 275 18 223 418 45 Cuts at: 2516 2520 2670 3018 3415 4304 5933 8 Size: 4 150 348 397 889 1629 43 Fragments arranged by size: 1629 1425 889 418 397 348 275 223 150 54 50 45 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2471 2516 2520 3415 1537 Size: 275 659 45 4 895 4090 Fragments arranged by size: 4090 895 659 275 45 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3898 1353 Size: 2545 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4555 4929 5075 387 Size: 1064 138 2966 374 146 1280 Fragments arranged by size: 2966 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2507 3251 4031 4628 5415 5683 1239 Size: 393 875 744 780 597 787 268 1524 Fragments arranged by size: 1524 875 787 780 744 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2028 2029 2281 2290 2977 3062 3165 2028 Size: 1 252 9 687 85 103 4831 Fragments arranged by size: 4831 687 252 103 85 9 1 EcoRI G'AATT_C Cuts at: 2137 3277 3655 2137 Size: 1140 378 4450 Fragments arranged by size: 4450 1140 378 FseI GG_CCGG'CC Cuts at: 2522 2522 Size: 5968 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 5115 1678 Size: 3437 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2544 2576 2855 Size: 32 135 32 525 32 415 32 279 Cuts at: 2855 2887 4609 4641 4683 4715 1373 Size: 32 1722 32 42 32 2626 Fragments arranged by size: 2626 1722 525 415 279 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2129 3312 3558 669 Size: 988 281 191 1183 246 3079 Fragments arranged by size: 3079 1183 988 281 246 191 HindIII A'AGCT_T Cuts at: 748 748 Size: 5968 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 2274 3567 2274 Size: 1293 4675 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5082 5266 1305 Size: 3777 184 2007 Fragments arranged by size: 3777 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 2672 10 Size: 54 1425 343 223 617 3306 Fragments arranged by size: 3306 1425 617 343 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 3926 4285 4444 518 Size: 648 2760 359 159 2042 Fragments arranged by size: 2760 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2941 3384 4061 5209 5454 1606 Size: 1335 443 677 1148 245 2120 Fragments arranged by size: 2120 1335 1148 677 443 245 NaeI GCC'GGC Cuts at: 2520 2520 Size: 5968 NcoI C'CATG_G Cuts at: 513 1065 2836 513 Size: 552 1771 3645 Fragments arranged by size: 3645 1771 552 NdeI CA'TA_TG Cuts at: 387 2956 387 Size: 2569 3399 NgoMIV G'CCGG_C Cuts at: 2518 2518 Size: 5968 NheI G'CTAG_C Cuts at: 1052 3089 1052 Size: 2037 3931 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 3251 3326 4871 1132 Size: 97 2022 75 1545 2229 Fragments arranged by size: 2229 2022 1545 97 75 PasI CC'CwG_GG Cuts at: 1348 2251 1348 Size: 903 5065 PciI A'CATG_T Cuts at: 4867 4867 Size: 5968 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2380 2401 1108 Size: 902 370 21 4675 Fragments arranged by size: 4675 902 370 21 PfoI T'CCnGG_A Cuts at: 2740 2740 Size: 5968 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4037 4069 5550 5582 4037 Size: 32 1481 32 4423 Fragments arranged by size: 4423 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 3062 3165 2281 Size: 781 103 5084 Fragments arranged by size: 5084 781 103 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PspOMI G'GGCC_C Cuts at: 2028 2028 Size: 5968 PstI C_TGCA'G Cuts at: 830 2226 2289 3211 3320 830 Size: 1396 63 922 109 3478 Fragments arranged by size: 3478 1396 922 109 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 PvuII CAG'CTG Cuts at: 2941 2941 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SanDI GG'GwC_CC Cuts at: 3062 3062 Size: 5968 SbfI CC_TGCA'GG Cuts at: 2289 3320 2289 Size: 1031 4937 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 2222 2285 3207 3316 4451 5132 5323 Size: 219 1177 63 922 109 1135 681 191 Cuts at: 5323 826 Size: 1471 Fragments arranged by size: 1471 1177 1135 922 681 219 191 109 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 3295 1872 Size: 1423 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4072 4973 5235 820 Size: 197 926 2129 901 262 1553 Fragments arranged by size: 2129 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 2131 152 Size: 1979 3989 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5968 StyI C'CwwG_G Cuts at: 513 1065 2836 3407 513 Size: 552 1771 571 3074 Fragments arranged by size: 3074 1771 571 552 SwaI ATTT'AAAT Cuts at: 2233 2233 Size: 5968 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3995 4012 4165 4350 4795 325 Size: 1255 2415 17 153 185 445 1498 Fragments arranged by size: 2415 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2482 4214 5926 Size: 274 80 33 51 1443 505 1732 1712 Cuts at: 5926 96 Size: 138 Fragments arranged by size: 1732 1712 1443 505 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3067 3099 5928 5960 3067 Size: 32 2829 32 3075 Fragments arranged by size: 3075 2829 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2728 1316 Size: 1412 4556 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2775 2857 2775 Size: 82 5886 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5968 XmaI C'CCGG_G Cuts at: 1870 3293 1870 Size: 1423 4545 XmnI GAAnn'nnTTC Cuts at: 4097 4097 Size: 5968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI