################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BcgI BclI BcnI BfaI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 1023 GTCTAC GT^[AC][GT]AC AccII 795 CGCG CG^CG AcyI 487 GGCGTC G[GA]^CG[CT]C AflIII 224 ACGTGT A^C[GA][CT]GT AlwNI 664 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 529 CCTGAGG CC^T[ACGT]AGG Aor51HI 392 AGCGCT AGC^GCT ApaI 674 GGGCCC GGGCC^C BalI 170 TGGCCA TGG^CCA BamHI 766 GGATCC G^GATCC BanI 574 GGCACC G^G[CT][GA]CC BanII 674 GGGCCC G[GA]GC[CT]^C BbvCI 669 GCTGAGG CCTCAGC(-5/-2) BciVI 292 GGATAC GTATCC(6/5) BglI 376 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 694 GCTCAGC GC^T[ACGT]AGC Bpu1102I 694 GCTCAGC GC^T[ACGT]AGC BsaHI 487 GGCGTC G[GA]^CG[CT]C BseRI 244 CTCCTC GAGGAG(10/8) BsiEI 306 CGGTCG CG[GA][CT]^CG BsmBI 489 CGTCTC CGTCTC(1/5) Bsp1407I 809 TGTACA T^GTACA BspMI 349 GCAGGT ACCTGC(4/8) BsrDI 81 CATTGC GCAATG(2/0) BsrFI 518 GCCGGT [GA]^CCGG[CT] BsrGI 809 TGTACA T^GTACA BstUI 795 CGCG CG^CG BstYI 766 GGATCC [GA]^GATC[CT] Bsu36I 529 CCTGAGG CC^T[ACGT]AGG CelII 694 GCTCAGC GC^T[ACGT]AGC Cfr10I 518 GCCGGT [GA]^CCGG[CT] DpnII 767 GATC ^GATC EaeI 170 TGGCCA [CT]^GGCC[GA] Eco47III 392 AGCGCT AGC^GCT Eco81I 529 CCTGAGG CC^T[ACGT]AGG HaeII 392 AGCGCT [GA]GCGC^[CT] HapII 519 CCGG C^CGG HgaI 488 GCGTC GACGC(5/10) Hin1I 487 GGCGTC G[GA]^CG[CT]C HindIII 932 AAGCTT A^AGCTT HpaII 519 CCGG C^CGG MboI 767 GATC ^GATC MflI 766 GGATCC [GA]^GATC[CT] MluNI 170 TGGCCA TGG^CCA MscI 170 TGGCCA TGG^CCA MspI 519 CCGG C^CGG MvnI 795 CGCG CG^CG NdeII 767 GATC ^GATC PflMI 831 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 453 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 538 CTGCAG CTGCA^G Sau3AI 767 GATC ^GATC SfiI 375 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 444 GCATGC GCATG^C SspBI 809 TGTACA T^GTACA TfiI 976 GAATC G^A[AT]TC Van91I 831 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 158 CCATGGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 766 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwI 767 GATCC GGATC(4/5) 766 GGATC AvaII 459 GGTCC G^G[AT]CC 473 GGACC BpmI 106 CTCCAG CTGGAG(16/14) 513 CTGGAG BsaAI 600 TACGTG [CT]AC^GT[GA] 784 TACGTG BsmAI 490 GTCTC GTCTC(1/5) 1018 GTCTC BspHI 914 TCATGA T^CATGA 941 TCATGA BsrI 372 ACTGG ACTGG(1/-1) 560 ACTGG BstXI 158 CCATGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 806 CCATGTACATGG CfoI 180 GCGC GCG^C 393 GCGC EcoT14I 158 CCATGG C^C[AT][AT]GG 831 CCATGG HhaI 180 GCGC GCG^C 393 GCGC HinP1I 180 GCGC G^CGC 393 GCGC HincII 220 GTCAAC GT[CT]^[GA]AC 817 GTCAAC HindII 220 GTCAAC GT[CT]^[GA]AC 817 GTCAAC HphI 738 GGTGA GGTGA(8/7) 854 TCACC MspA1I 282 CAGCTG C[AC]G^C[GT]G 664 CAGCTG NcoI 158 CCATGG C^CATGG 831 CCATGG NspI 444 GCATGC [GA]CATG^[CT] 985 ACATGC PleI 592 GAGTC GAGTC(4/5) 706 GAGTC PvuII 282 CAGCTG CAG^CTG 664 CAGCTG RcaI 914 TCATGA T^CATGA 941 TCATGA SfcI 344 CTGTAG C^T[GA][CT]AG 538 CTGCAG StyI 158 CCATGG C^C[AT][AT]GG 831 CCATGG Tsp45I 369 GTCAC ^GT[GC]AC 853 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeII MaeIII MboII MnlI MslI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI TseI TspRI