(Circular) (Six-base) MAPSORT of: pFN21AE9524.seq Check: 3693 from: 1 to: 5749 pFN21AE9524 5749 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3029 3092 1937 Size: 1092 63 4594 Fragments arranged by size: 4594 1092 63 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3048 3876 4249 1563 Size: 1485 828 373 3063 Fragments arranged by size: 3063 1485 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2037 2422 3812 5196 5464 1632 Size: 405 385 1390 1384 268 1917 Fragments arranged by size: 1917 1390 1384 405 385 268 AfeI AGC'GCT Cuts at: 2399 2399 Size: 5749 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 2229 4648 5625 1203 Size: 1026 2419 977 1327 Fragments arranged by size: 2419 1327 1026 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2674 3492 5064 2674 Size: 818 1572 3359 Fragments arranged by size: 3359 1572 818 ApaI G_GGCC'C Cuts at: 2683 2683 Size: 5749 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 3074 1870 Size: 73 1131 4545 Fragments arranged by size: 4545 1131 73 BamHI G'GATC_C Cuts at: 2771 3079 3446 2771 Size: 308 367 5074 Fragments arranged by size: 5074 367 308 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 3070 4525 618 Size: 325 217 627 792 491 1455 1842 Fragments arranged by size: 1842 1455 792 627 491 325 217 BanII G_rGCy'C Cuts at: 1391 2683 3068 1391 Size: 1292 385 4072 Fragments arranged by size: 4072 1292 385 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2128 2260 2269 2299 928 Size: 971 229 132 9 30 4378 Fragments arranged by size: 4378 971 229 132 30 9 BbvCI CC'TCA_GC Cuts at: 2675 2675 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2291 3641 4851 2291 Size: 1350 1210 3189 Fragments arranged by size: 3189 1350 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2387 4360 136 Size: 107 122 71 631 605 715 1973 1525 Fragments arranged by size: 1973 1525 715 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 2700 3166 2700 Size: 466 5283 Bme1580I G_kGCm'C Cuts at: 1790 2132 2348 2582 2683 2796 3810 4966 1790 Size: 342 216 234 101 113 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 342 234 216 113 101 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2264 2264 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2539 4409 1239 Size: 857 443 1870 2579 Fragments arranged by size: 2579 1870 857 443 Bpu10I CC'TnA_GC Cuts at: 2675 2675 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2607 2791 3540 493 Size: 711 1403 184 749 2702 Fragments arranged by size: 2702 1403 749 711 184 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 3556 3938 4573 Size: 53 83 186 936 960 1063 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1063 960 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 1178 Size: 30 165 30 609 30 4885 Fragments arranged by size: 4885 609 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 1186 Size: 1054 4695 BseYI C'CCAG_C Cuts at: 4952 5448 4952 Size: 496 5253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2314 3199 3960 4988 1540 Size: 275 186 313 885 761 1028 2301 Fragments arranged by size: 2301 1028 885 761 313 275 186 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 BsmBI CGTCTCn'nnnn_ Cuts at: 2500 2500 Size: 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2348 2582 2683 2796 3068 3810 Size: 399 342 216 234 101 113 272 742 Cuts at: 3810 3895 4966 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 342 272 234 216 113 101 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 2919 2946 3638 5368 2919 Size: 27 692 1730 3300 Fragments arranged by size: 3300 1730 692 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2345 3104 844 Size: 1501 759 3489 Fragments arranged by size: 3489 1501 759 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2085 4244 4418 65 Size: 1183 837 2159 174 1396 Fragments arranged by size: 2159 1396 1183 837 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 4393 4580 1700 Size: 110 713 1870 187 2869 Fragments arranged by size: 2869 1870 713 187 110 BsrGI T'GTAC_A Cuts at: 96 2814 96 Size: 2718 3031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2818 2170 Size: 648 5101 BstYI r'GATC_y Cuts at: 1972 2771 3079 3446 3831 3848 5300 5386 5398 Size: 799 308 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 799 385 367 346 308 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2535 2535 Size: 5749 BtgI C'CryG_G Cuts at: 513 1065 2063 2163 2574 2836 513 Size: 552 998 100 411 262 3426 Fragments arranged by size: 3426 998 552 411 262 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2180 2552 3265 4059 4079 2180 Size: 372 713 794 20 3850 Fragments arranged by size: 3850 794 713 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 3196 4085 Size: 54 1425 50 275 18 345 1021 889 Cuts at: 4085 5714 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1021 889 345 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2019 2056 2621 3679 1353 Size: 666 37 565 1058 3423 Fragments arranged by size: 3423 1058 666 565 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2037 2096 2422 2539 3812 4409 5196 Size: 393 405 59 326 117 1273 597 787 Cuts at: 5196 5464 1239 Size: 268 1524 Fragments arranged by size: 1524 1273 787 597 405 393 326 268 117 59 EcoO109I rG'GnC_Cy Cuts at: 2539 2679 2680 2539 Size: 140 1 5608 Fragments arranged by size: 5608 140 1 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2401 4896 1678 Size: 723 2495 2531 Fragments arranged by size: 2531 2495 723 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2289 2321 4390 4422 4464 Size: 32 135 32 717 32 2069 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 2069 717 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2824 3093 3339 669 Size: 988 281 289 597 269 246 3079 Fragments arranged by size: 3079 988 597 289 281 269 246 HindIII A'AGCT_T Cuts at: 748 2937 748 Size: 2189 3560 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1998 3009 4863 5047 1305 Size: 693 1011 1854 184 2007 Fragments arranged by size: 2007 1854 1011 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 10 Size: 54 1425 343 345 3582 Fragments arranged by size: 3582 1425 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2322 2816 2951 3707 4066 4225 518 Size: 648 1156 494 135 756 359 159 2042 Fragments arranged by size: 2042 1156 756 648 494 359 159 135 MspA1I CmG'CkG Cuts at: 1606 2289 2671 3165 3842 4990 5235 1606 Size: 683 382 494 677 1148 245 2120 Fragments arranged by size: 2120 1148 683 677 494 382 245 NcoI C'CATG_G Cuts at: 513 1065 2163 2836 513 Size: 552 1098 673 3426 Fragments arranged by size: 3426 1098 673 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2453 2994 3107 4652 1132 Size: 97 1224 541 113 1545 2229 Fragments arranged by size: 2229 1545 1224 541 113 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2842 1108 Size: 1734 4015 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2462 1613 Size: 849 4900 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PspOMI G'GGCC_C Cuts at: 2679 2679 Size: 5749 PstI C_TGCA'G Cuts at: 830 2547 3101 830 Size: 1717 554 3478 Fragments arranged by size: 3478 1717 554 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2289 2671 2289 Size: 382 5367 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2349 2543 3097 4232 4913 5104 826 Size: 219 1304 194 554 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 681 554 219 194 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2387 2387 Size: 5749 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 2453 3107 1132 Size: 1321 654 3774 Fragments arranged by size: 3774 1321 654 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2163 2836 3188 513 Size: 552 1098 673 352 3074 Fragments arranged by size: 3074 1098 673 552 352 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2300 3776 3793 3946 4131 4576 325 Size: 1255 720 1476 17 153 185 445 1498 Fragments arranged by size: 1498 1476 1255 720 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2814 3995 5707 Size: 274 80 33 51 1443 837 1181 1712 Cuts at: 5707 96 Size: 138 Fragments arranged by size: 1712 1443 1181 837 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2367 2399 5709 5741 2367 Size: 32 3310 32 2375 Fragments arranged by size: 3310 2375 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2170 2170 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI