################################## # NO CUT ENZYMES ( 208 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssKI BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MroI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 138 GTATAC GT^[AC][GT]AC AfaI 81 GTAC GT^AC AluI 164 AGCT AG^CT BalI 340 TGGCCA TGG^CCA BbvI 124 GCAGC GCAGC(8/12) BseRI 56 CTCCTC GAGGAG(10/8) BsiYI 326 CCGATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 326 CCGATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1407I 80 TGTACA T^GTACA BsrGI 80 TGTACA T^GTACA BsrI 25 ACTGG ACTGG(1/-1) BssSI 61 CACGAG CACGAG(-5/-1) Bst1107I 138 GTATAC GTA^TAC BstZ17I 138 GTATAC GTA^TAC Cac8I 165 GCTGGC GC[ACGT]^[ACGT]GC DdeI 70 CTAAG C^T[ACGT]AG DpnII 328 GATC ^GATC EaeI 340 TGGCCA [CT]^GGCC[GA] FauI 4 GCGGG CCCGC(4/6) Fnu4HI 124 GCAGC GC^[ACGT]GC HphI 237 GGTGA GGTGA(8/7) ItaI 124 GCAGC GC^[ACGT]GC MboI 328 GATC ^GATC MluNI 340 TGGCCA TGG^CCA MscI 340 TGGCCA TGG^CCA NdeII 328 GATC ^GATC NlaIII 256 CATG CATG^ RsaI 81 GTAC GT^AC SapI 313 GAAGAGC GCTCTTC(1/4) Sau3AI 328 GATC ^GATC SmlI 103 CTTGAG C^T[CT][GA]AG SspBI 80 TGTACA T^GTACA SspI 75 AATATT AAT^ATT TseI 124 GCAGC G^C[AT]GC XcmI 23 CCACTGGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbsI 18 GAAGAC GAAGAC(2/6) 205 GAAGAC BpuAI 18 GAAGAC GAAGAC(2/6) 205 GAAGAC EarI 232 GAAGAG CTCTTC(1/4) 313 GAAGAG HaeIII 28 GGCC GG^CC 341 GGCC HinfI 90 GATTC G^A[ACGT]TC 240 GATTC Ksp632I 232 GAAGAG CTCTTC(1/4) 313 GAAGAG MseI 176 TTAA T^TAA 194 TTAA NlaIV 183 GGAACC GG[ACGT]^[ACGT]CC 300 GGAGCC TfiI 90 GATTC G^A[AT]TC 240 GATTC Tru9I 176 TTAA T^TAA 194 TTAA Tsp509I 200 AATT ^AATT 227 AATT TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^ 331 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AciI MboII MnlI