################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 919 CGCG CG^CG AciI 1720 CCGC CCGC(-3/-1) AfaI 1378 GTAC GT^AC AflII 504 CTTAAG C^TTAAG AhdI 709 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 415 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 922 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 709 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 1102 GGACC G^G[AT]CC BanII 967 GAGCCC G[GA]GC[CT]^C BclI 17 TGATCA T^GATCA BcnI 1702 CCGGG CC^[GC]GG BfrI 504 CTTAAG C^TTAAG BglII 1860 AGATCT A^GATCT BlpI 423 GCTCAGC GC^T[ACGT]AGC Bpu1102I 423 GCTCAGC GC^T[ACGT]AGC BsgI 1396 CTGCAC GTGCAG(16/14) BsmI 1371 GAATGC GAATGC(1/-1) BspMI 879 GCAGGT ACCTGC(4/8) BstUI 919 CGCG CG^CG CelII 423 GCTCAGC GC^T[ACGT]AGC DraI 813 TTTAAA TTT^AAA DsaI 756 CCATGG C^C[GA][CT]GG Eam1105I 709 GACAGCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1632 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1619 ATGCAT ATGCA^T FbaI 17 TGATCA T^GATCA HapII 1702 CCGG C^CGG HgaI 435 GCGTC GACGC(5/10) HincII 1296 GTTAAC GT[CT]^[GA]AC HindII 1296 GTTAAC GT[CT]^[GA]AC HpaI 1296 GTTAAC GTT^AAC HpaII 1702 CCGG C^CGG MfeI 349 CAATTG C^AATTG MslI 1176 CATTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 1317 CAGCTG C[AC]G^C[GT]G MspI 1702 CCGG C^CGG MunI 349 CAATTG C^AATTG MvnI 919 CGCG CG^CG NciI 1702 CCGGG CC^[GC]GG NcoI 756 CCATGG C^CATGG NsiI 1619 ATGCAT ATGCA^T NspI 953 ACATGC [GA]CATG^[CT] PpuMI 1101 GGGACCC [GA]G^G[AT]CC[CT] PvuII 1317 CAGCTG CAG^CTG RsaI 1378 GTAC GT^AC TfiI 519 GATTC G^A[AT]TC XmnI 922 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 1210 GAATTT [GA]^AATT[CT] 1310 AAATTT AlwI 42 GATCC GGATC(4/5) 1671 GGATC ApoI 1210 GAATTT [GA]^AATT[CT] 1310 AAATTT BfaI 589 CTAG C^TAG 1004 CTAG BmyI 967 GAGCCC G[AGT]GC[ACT]^C 1129 GTGCCC BsmFI 441 GGGAC GGGAC(10/14) 1101 GGGAC Bsp1286I 967 GAGCCC G[AGT]GC[ACT]^C 1129 GTGCCC BsrDI 934 GCAATG GCAATG(2/0) 1685 GCAATG BstAPI 414 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 879 GCAGGTGGTGC BstYI 41 AGATCC [GA]^GATC[CT] 1860 AGATCT DraII 733 GGGGCCT [GA]G^G[ACGT]CC[CT] 1101 GGGACCC EarI 1747 GAAGAG CTCTTC(1/4) 1843 GAAGAG EcoO109I 733 GGGGCCT [GA]G^G[ACGT]CC[CT] 1101 GGGACCC EcoT14I 756 CCATGG C^C[AT][AT]GG 1568 CCTTGG Ksp632I 1747 GAAGAG CTCTTC(1/4) 1843 GAAGAG MaeI 589 CTAG C^TAG 1004 CTAG MflI 41 AGATCC [GA]^GATC[CT] 1860 AGATCT PstI 112 CTGCAG CTGCA^G 1636 CTGCAG SapI 1747 GAAGAGC GCTCTTC(1/4) 1843 GAAGAGC SfcI 112 CTGCAG C^T[GA][CT]AG 1636 CTGCAG SmlI 494 CTCAAG C^T[CT][GA]AG 504 CTTAAG StyI 756 CCATGG C^C[AT][AT]GG 1568 CCTTGG XhoII 41 AGATCC [GA]^GATC[CT] 1860 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AluI BalI BbsI BbvCI BbvI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I