(Circular) (Six-base) MAPSORT of: pFN21AE5741.seq Check: 5141 from: 1 to: 6574 pFN21AE5741 6574 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2875 2875 Size: 6574 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4384 278 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2156 2420 2676 3334 3882 4637 6021 Size: 404 120 264 256 658 548 755 1384 Cuts at: 6021 6289 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 755 658 548 404 268 264 256 120 AflII C'TTAA_G Cuts at: 820 1017 2509 820 Size: 197 1492 4885 Fragments arranged by size: 4885 1492 197 AflIII A'CryG_T Cuts at: 1203 5473 6450 1203 Size: 4270 977 1327 Fragments arranged by size: 4270 1327 977 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 2719 5303 2719 Size: 2584 3990 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AlwNI CAG_nnn'CTG Cuts at: 2425 4317 5889 2425 Size: 1892 1572 3110 Fragments arranged by size: 3110 1892 1572 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 3215 3315 3883 4109 4261 6370 1493 Size: 1722 100 568 226 152 2109 1697 Fragments arranged by size: 2109 1722 1697 568 226 152 100 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 3899 1870 Size: 73 1956 4545 Fragments arranged by size: 4545 1956 73 BamHI G'GATC_C Cuts at: 3904 4271 3904 Size: 367 6207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3895 5350 618 Size: 325 217 627 2108 1455 1842 Fragments arranged by size: 2108 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2976 3893 1391 Size: 1585 917 4072 Fragments arranged by size: 4072 1585 917 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2368 2574 2682 2924 3663 3783 3834 Size: 971 469 206 108 242 739 120 51 Cuts at: 3834 928 Size: 3668 Fragments arranged by size: 3668 971 739 469 242 206 120 108 51 BbvCI CC'TCA_GC Cuts at: 2031 2073 3720 2031 Size: 42 1647 4885 Fragments arranged by size: 4885 1647 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4466 5676 4466 Size: 1210 5364 BclI T'GATC_A Cuts at: 1548 2022 1548 Size: 474 6100 BfrBI ATG'CAT Cuts at: 3626 3626 Size: 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5185 136 Size: 107 122 71 631 605 3513 1525 Fragments arranged by size: 3513 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3865 6569 3865 Size: 2704 3870 BlpI GC'TnA_GC Cuts at: 2429 3991 2429 Size: 1562 5012 Bme1580I G_kGCm'C Cuts at: 1790 3138 4635 5791 1790 Size: 1348 1497 1156 2573 Fragments arranged by size: 2573 1497 1348 1156 BmgBI CAC'GTC Cuts at: 1318 3745 1318 Size: 2427 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2745 3080 4305 Size: 416 53 83 399 24 1328 335 1225 Cuts at: 4305 5263 442 Size: 958 1753 Fragments arranged by size: 1753 1328 1225 958 416 399 335 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3075 3075 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2393 2571 5234 1239 Size: 1139 15 178 2663 2579 Fragments arranged by size: 2663 2579 1139 178 15 Bpu10I CC'TnA_GC Cuts at: 2031 2073 3720 2031 Size: 42 1647 4885 Fragments arranged by size: 4885 1647 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2484 4699 5564 5862 2484 Size: 2215 865 298 3196 Fragments arranged by size: 3196 2215 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5237 882 Size: 617 3738 2219 Fragments arranged by size: 3738 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4365 493 Size: 711 3161 2702 Fragments arranged by size: 3161 2702 711 BsaBI GATnn'nnATC Cuts at: 6568 6568 Size: 6574 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4381 4763 5398 275 Size: 53 83 186 936 2848 382 635 1451 Fragments arranged by size: 2848 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5007 5405 5679 5826 6237 5007 Size: 398 274 147 411 5344 Fragments arranged by size: 5344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2600 2630 1178 Size: 30 165 30 1197 30 5122 Fragments arranged by size: 5122 1197 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2061 2608 2620 2744 1186 Size: 875 547 12 124 5016 Fragments arranged by size: 5016 875 547 124 12 BseYI C'CCAG_C Cuts at: 2364 3124 5777 6273 2364 Size: 760 2653 496 2665 Fragments arranged by size: 2665 2653 760 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3386 1163 Size: 573 1650 4351 Fragments arranged by size: 4351 1650 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4635 4720 5791 4635 Size: 85 1071 5418 Fragments arranged by size: 5418 1071 85 BsmI GAATG_Cn' Cuts at: 3382 4085 4178 3382 Size: 703 93 5778 Fragments arranged by size: 5778 703 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2976 3138 3893 4635 4720 5791 1391 Size: 399 1186 162 755 742 85 1071 2174 Fragments arranged by size: 2174 1186 1071 755 742 399 162 85 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 4463 6193 4463 Size: 1730 4844 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2875 3929 844 Size: 2031 1054 3489 Fragments arranged by size: 3489 2031 1054 BsrBI CCG'CTC Cuts at: 1394 1823 4461 1394 Size: 429 2638 3507 Fragments arranged by size: 3507 2638 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2946 3697 5069 5243 65 Size: 1183 1698 751 1372 174 1396 Fragments arranged by size: 1698 1396 1372 1183 751 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5218 5405 1700 Size: 110 3408 187 2869 Fragments arranged by size: 3408 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6574 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 2425 2890 794 Size: 1631 465 4478 Fragments arranged by size: 4478 1631 465 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstYI r'GATC_y Cuts at: 1972 2046 3865 3904 4271 4656 4673 6125 6211 Size: 74 1819 39 367 385 17 1452 86 Cuts at: 6211 6223 6569 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1819 1452 385 367 346 86 74 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2761 513 Size: 552 1696 4326 Fragments arranged by size: 4326 1696 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 2859 4090 4884 4904 2859 Size: 1231 794 20 4529 Fragments arranged by size: 4529 1231 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 2820 3878 2820 Size: 1058 5516 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2467 2758 2777 Size: 54 1425 50 275 18 637 291 19 Cuts at: 2777 4021 4910 6539 8 Size: 1244 889 1629 43 Fragments arranged by size: 1629 1425 1244 889 637 291 275 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3747 3843 4504 1353 Size: 2394 96 661 3423 Fragments arranged by size: 3423 2394 661 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2156 2378 2393 2420 2571 2676 Size: 393 404 120 222 15 27 151 105 Cuts at: 2676 3334 3882 4637 5234 6021 6289 1239 Size: 658 548 755 597 787 268 1524 Fragments arranged by size: 1524 787 755 658 597 548 404 393 268 222 151 120 105 27 15 EcoNI CCTnn'n_nnAGG Cuts at: 3641 3641 Size: 6574 EcoO109I rG'GnC_Cy Cuts at: 2739 3107 2739 Size: 368 6206 EcoRI G'AATT_C Cuts at: 3883 4261 3883 Size: 378 6196 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 5721 1678 Size: 4043 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2014 2046 2705 2737 3842 Size: 32 135 32 442 32 659 32 1105 Cuts at: 3842 3874 5215 5247 5289 5321 1373 Size: 32 1341 32 42 32 2626 Fragments arranged by size: 2626 1341 1105 659 442 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3918 4164 669 Size: 988 281 1365 615 246 3079 Fragments arranged by size: 3079 1365 988 615 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6574 HpaI GTT'AAC Cuts at: 3303 4164 3303 Size: 861 5713 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 2354 4173 2354 Size: 1819 4755 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2119 2658 3015 3527 5688 5872 1305 Size: 814 539 357 512 2161 184 2007 Fragments arranged by size: 2161 2007 814 539 512 357 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2469 2760 2779 10 Size: 54 1425 343 637 291 19 3805 Fragments arranged by size: 3805 1425 637 343 291 54 19 MslI CAynn'nnrTG Cuts at: 518 1166 3185 4532 4891 5050 518 Size: 648 2019 1347 359 159 2042 Fragments arranged by size: 2042 2019 1347 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3324 3990 4667 5815 6060 1606 Size: 1718 666 677 1148 245 2120 Fragments arranged by size: 2120 1718 1148 677 666 245 NcoI C'CATG_G Cuts at: 513 1065 2761 513 Size: 552 1696 4326 Fragments arranged by size: 4326 1696 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6574 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NsiI A_TGCA'T Cuts at: 3628 3628 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2962 3932 5477 1132 Size: 97 1733 970 1545 2229 Fragments arranged by size: 2229 1733 1545 970 97 PasI CC'CwG_GG Cuts at: 1348 3103 1348 Size: 1755 4819 PciI A'CATG_T Cuts at: 5473 5473 Size: 6574 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6574 PfoI T'CCnGG_A Cuts at: 2305 3607 2305 Size: 1302 5272 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4643 4675 6156 6188 4643 Size: 32 1481 32 5029 Fragments arranged by size: 5029 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3107 3107 Size: 6574 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 3222 4144 3222 Size: 922 5652 PstI C_TGCA'G Cuts at: 830 2121 3645 3926 830 Size: 1291 1524 281 3478 Fragments arranged by size: 3478 1524 1291 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 3324 3324 Size: 6574 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SanDI GG'GwC_CC Cuts at: 3107 3107 Size: 6574 SapI GCTCTTCn'nnn_ Cuts at: 3747 3843 3747 Size: 96 6478 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SfcI C'TryA_G Cuts at: 826 1045 2117 3641 3922 5057 5738 5929 826 Size: 219 1072 1524 281 1135 681 191 1471 Fragments arranged by size: 1524 1471 1135 1072 681 281 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2499 2509 4678 5579 5841 820 Size: 197 926 556 10 2169 901 262 1553 Fragments arranged by size: 2169 1553 926 901 556 262 197 10 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 4498 5 Size: 47 4446 2081 Fragments arranged by size: 4446 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 2761 3573 4013 513 Size: 552 1696 812 440 3074 Fragments arranged by size: 3074 1696 812 552 440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2868 4601 4618 4771 4956 5401 325 Size: 1255 1288 1733 17 153 185 445 1498 Fragments arranged by size: 1733 1498 1288 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4820 6532 96 Size: 274 80 33 51 1443 2843 1712 138 Fragments arranged by size: 2843 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 6534 6566 2469 Size: 32 4033 32 2477 Fragments arranged by size: 4033 2477 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 2931 4703 2931 Size: 1772 4802 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4382 276 Size: 53 83 186 936 2848 2468 Fragments arranged by size: 2848 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI