################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI AocI ApaI AscI Asp718I AspEI AspI AviII AvrII BamHI BbvCI BciVI BglI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoT22I FspI KpnI KspI MamI MluI MroI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflII 1747 CTTAAG C^TTAAG AflIII 1448 ACATGT A^C[GA][CT]GT Alw44I 502 GTGCAC G^TGCAC ApaLI 502 GTGCAC G^TGCAC AseI 2932 ATTAAT AT^TAAT AsnI 2932 ATTAAT AT^TAAT AvaI 393 CCCGGG C^[CT]CG[GA]G BfrI 1747 CTTAAG C^TTAAG BsaWI 386 ACCGGA [AT]^CCGG[AT] BsiEI 1071 CGACCG CG[GA][CT]^CG BsmI 3113 GAATGC GAATGC(1/-1) BsoBI 393 CCCGGG C^[CT]CG[GA]G BspLU11I 1448 ACATGT A^CATGT BsrDI 1926 CATTGC GCAATG(2/0) BssSI 1036 CACGAG CACGAG(-5/-1) BstEII 540 GGTGACC G^GT[ACGT]ACC BstPI 540 GGTGACC G^GT[ACGT]ACC BstXI 1550 CCATTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 406 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 540 GGTGACC G^GT[ACGT]ACC EcoRI 2325 GAATTC G^AATTC FseI 183 GGCCGGCC GGCCGG^CC NaeI 184 GCCGGC GCC^GGC NcoI 101 CCATGG C^CATGG NgoMIV 184 GCCGGC G^CCGGC PshBI 2932 ATTAAT AT^TAAT SacI 106 GAGCTC GAGCT^C SmaI 393 CCCGGG CCC^GGG SspI 2851 AATATT AAT^ATT StuI 1331 AGGCCT AGG^CCT XmaCI 393 CCCGGG C^CCGGG XmaI 393 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Aor51HI 231 AGCGCT AGC^GCT 2382 AGCGCT Asp700I 901 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2755 GAAAAGTTTC AspHI 106 GAGCTC G[AT]GC[AT]^C 502 GTGCAC BbeI 56 GGCGCC GGCGC^C 724 GGCGCC BbrPI 178 CACGTG CAC^GTG 910 CACGTG BcgI 1577 CGATAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 656 GCATCTTCATCG BfaI 943 CTAG C^TAG 3052 CTAG BglII 323 AGATCT A^GATCT 1295 AGATCT BsaAI 178 CACGTG [CT]AC^GT[GA] 910 CACGTG BsaI 238 GAGACC GGTCTC(1/5) 702 GGTCTC BsiHKAI 106 GAGCTC G[AT]GC[AT]^C 502 GTGCAC BspMI 1394 ACCTGC ACCTGC(4/8) 2318 ACCTGC EarI 901 GAAGAG CTCTTC(1/4) 961 GAAGAG Eco47III 231 AGCGCT AGC^GCT 2382 AGCGCT EcoNI 942 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2156 CCTATCTGAGG EcoRV 1105 GATATC GAT^ATC 1458 GATATC FauI 423 CCCGC CCCGC(4/6) 546 CCCGC HgaI 2650 GACGC GACGC(5/10) 818 GCGTC HincII 1875 GTTAAC GT[CT]^[GA]AC 3123 GTTAAC HindII 1875 GTTAAC GT[CT]^[GA]AC 3123 GTTAAC HpaI 1875 GTTAAC GTT^AAC 3123 GTTAAC KasI 56 GGCGCC G^GCGCC 724 GGCGCC Ksp632I 901 GAAGAG CTCTTC(1/4) 961 GAAGAG MaeI 943 CTAG C^TAG 3052 CTAG NarI 56 GGCGCC GG^CGCC 724 GGCGCC NdeI 2357 CATATG CA^TATG 2597 CATATG NspI 1448 ACATGT [GA]CATG^[CT] 2522 ACATGC PflMI 264 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1824 CCAATTGTTGG PmaCI 178 CACGTG CAC^GTG 910 CACGTG PmlI 178 CACGTG CAC^GTG 910 CACGTG SfoI 56 GGCGCC GGC^GCC 724 GGCGCC Van91I 264 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1824 CCAATTGTTGG XmnI 901 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2755 GAAAAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DsaI EaeI EcoRII EcoT14I FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII