(Circular) (Six-base) MAPSORT of: pFN21AE3371.seq Check: 9957 from: 1 to: 8011 pFN21AE3371 8011 bp With 182 enzymes: SgfI * February 3, 2015 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2490 2499 2615 4281 Size: 53 83 186 936 954 9 116 1666 Cuts at: 4281 5821 278 Size: 1540 2468 Fragments arranged by size: 2468 1666 1540 954 936 186 116 83 53 9 AccI GT'mk_AC Cuts at: 1937 5354 1937 Size: 3417 4594 Acc65I G'GTAC_C Cuts at: 5332 5332 Size: 8011 AclI AA'CG_TT Cuts at: 1563 6138 6511 1563 Size: 4575 373 3063 Fragments arranged by size: 4575 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4070 4776 6074 7458 7726 1632 Size: 2438 706 1298 1384 268 1917 Fragments arranged by size: 2438 1917 1384 1298 706 268 AfeI AGC'GCT Cuts at: 2238 4389 2238 Size: 2151 5860 AflII C'TTAA_G Cuts at: 820 1017 3752 820 Size: 197 2735 5079 Fragments arranged by size: 5079 2735 197 AflIII A'CryG_T Cuts at: 1203 3453 6910 7887 1203 Size: 2250 3457 977 1327 Fragments arranged by size: 3457 2250 1327 977 AgeI A'CCGG_T Cuts at: 6842 6842 Size: 8011 AhdI GACnn_n'nnGTC Cuts at: 6740 6740 Size: 8011 AleI CACnn'nnGTG Cuts at: 1166 3045 1166 Size: 1879 6132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4202 1184 Size: 3018 4993 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3530 3562 3530 Size: 32 7979 AlwNI CAG_nnn'CTG Cuts at: 2359 3895 3946 5754 7326 2359 Size: 1536 51 1808 1572 3044 Fragments arranged by size: 3044 1808 1572 1536 51 ApaLI G'TGCA_C Cuts at: 2507 6068 7224 2507 Size: 3561 1156 3294 Fragments arranged by size: 3561 3294 1156 ApoI r'AATT_y Cuts at: 1493 4007 4330 4674 5027 5320 5546 5698 7807 Size: 2514 323 344 353 293 226 152 2109 Cuts at: 7807 1493 Size: 1697 Fragments arranged by size: 2514 2109 1697 353 344 323 293 226 152 AseI AT'TA_AT Cuts at: 160 1032 4938 6565 160 Size: 872 3906 1627 1606 Fragments arranged by size: 3906 1627 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8011 AvaI C'yCGr_G Cuts at: 1870 1943 2398 5336 1870 Size: 73 455 2938 4545 Fragments arranged by size: 4545 2938 455 73 BamHI G'GATC_C Cuts at: 5341 5708 5341 Size: 367 7644 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2061 2424 2729 5332 6787 Size: 325 217 627 274 363 305 2603 1455 Cuts at: 6787 618 Size: 1842 Fragments arranged by size: 2603 1842 1455 627 363 325 305 274 217 BanII G_rGCy'C Cuts at: 1391 2115 2165 2399 3286 5330 1391 Size: 724 50 234 887 2044 4072 Fragments arranged by size: 4072 2044 887 724 234 50 BbeI G_GCGC'C Cuts at: 2065 2733 2065 Size: 668 7343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3486 5012 5148 928 Size: 971 1587 1526 136 3791 Fragments arranged by size: 3791 1587 1526 971 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2650 2684 3571 3605 6202 6236 1903 Size: 34 713 34 887 34 2597 34 3678 Fragments arranged by size: 3678 2597 887 713 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5903 7113 5903 Size: 1210 6801 BclI T'GATC_A Cuts at: 1548 4863 5085 5212 1548 Size: 3315 222 127 4347 Fragments arranged by size: 4347 3315 222 127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6622 136 Size: 107 122 71 631 605 4950 1525 Fragments arranged by size: 4950 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2328 3300 8006 2328 Size: 972 4706 2333 Fragments arranged by size: 4706 2333 972 BlpI GC'TnA_GC Cuts at: 5428 5428 Size: 8011 Bme1580I G_kGCm'C Cuts at: 1790 2511 4905 6072 7228 1790 Size: 721 2394 1167 1156 2573 Fragments arranged by size: 2573 2394 1167 1156 721 BmgBI CAC'GTC Cuts at: 1318 3513 1318 Size: 2195 5816 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3348 4111 5119 Size: 416 53 83 399 24 1931 763 1008 Cuts at: 5119 5742 6700 442 Size: 623 958 1753 Fragments arranged by size: 1931 1753 1008 958 763 623 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8011 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2261 2543 2919 3281 3303 3968 6671 Size: 794 228 282 376 362 22 665 2703 Cuts at: 6671 1239 Size: 2579 Fragments arranged by size: 2703 2579 794 665 376 362 282 228 22 Bpu10I CC'TnA_GC Cuts at: 3594 3774 3594 Size: 180 7831 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 3266 4120 4463 6136 7001 7299 2879 Size: 387 854 343 1673 865 298 3591 Fragments arranged by size: 3591 1673 865 854 387 343 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 2713 6674 882 Size: 617 738 476 3961 2219 Fragments arranged by size: 3961 2219 738 617 476 BsaAI yAC'GTr Cuts at: 493 1204 2185 2917 5802 493 Size: 711 981 732 2885 2702 Fragments arranged by size: 2885 2702 981 732 711 BsaBI GATnn'nnATC Cuts at: 8005 8005 Size: 8011 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 2487 2496 2612 Size: 53 83 186 936 529 425 9 116 Cuts at: 2612 2730 4278 4656 5818 6200 6835 275 Size: 118 1548 378 1162 382 635 1451 Fragments arranged by size: 1548 1451 1162 936 635 529 425 382 378 186 118 116 83 53 9 BsaWI w'CCGG_w Cuts at: 2391 6444 6842 7116 7263 7674 2391 Size: 4053 398 274 147 411 2728 Fragments arranged by size: 4053 2728 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 2621 2651 3215 Size: 30 165 30 1014 30 174 30 564 Cuts at: 3215 3245 3279 3309 3429 3459 1178 Size: 30 34 30 120 30 5730 Fragments arranged by size: 5730 1014 564 174 165 120 34 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 3236 5201 1186 Size: 1267 783 1965 3996 Fragments arranged by size: 3996 1965 1267 783 BseYI C'CCAG_C Cuts at: 2275 7214 7710 2275 Size: 4939 496 2576 Fragments arranged by size: 4939 2576 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2165 2372 2438 2974 4175 4821 5270 Size: 573 429 207 66 536 1201 646 449 Cuts at: 5270 1163 Size: 3904 Fragments arranged by size: 3904 1201 646 573 536 449 429 207 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 5461 6222 7250 1540 Size: 275 186 1078 2382 761 1028 2301 Fragments arranged by size: 2382 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2511 6072 6157 7228 2115 Size: 396 3561 85 1071 2898 Fragments arranged by size: 3561 2898 1071 396 85 BsmI GAATG_Cn' Cuts at: 5124 5522 5615 5124 Size: 398 93 7520 Fragments arranged by size: 7520 398 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2165 2399 2511 3286 4905 5330 Size: 399 325 50 234 112 775 1619 425 Cuts at: 5330 6072 6157 7228 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1619 1071 775 742 425 399 325 234 112 85 50 BspEI T'CCGG_A Cuts at: 7674 7674 Size: 8011 BspHI T'CATG_A Cuts at: 5900 7630 5900 Size: 1730 6281 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3408 4332 5366 844 Size: 2564 924 1034 3489 Fragments arranged by size: 3489 2564 1034 924 BsrBI CCG'CTC Cuts at: 1394 1823 5898 1394 Size: 429 4075 3507 Fragments arranged by size: 4075 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3930 6506 6680 65 Size: 1183 2682 2576 174 1396 Fragments arranged by size: 2682 2576 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2189 2271 3002 6655 6842 1700 Size: 110 379 82 731 3653 187 2869 Fragments arranged by size: 3653 2869 731 379 187 110 82 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8011 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8011 BssSI C'ACGA_G Cuts at: 3041 6071 7083 3041 Size: 3030 1012 3969 Fragments arranged by size: 3969 3030 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8011 BstBI TT'CG_AA Cuts at: 6815 6815 Size: 8011 BstEII G'GTnAC_C Cuts at: 1184 2545 1184 Size: 1361 6650 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3562 2011 Size: 1551 6460 BstYI r'GATC_y Cuts at: 1972 2328 2406 3300 4585 5341 5708 6093 6110 Size: 356 78 894 1285 756 367 385 17 Cuts at: 6110 7562 7648 7660 8006 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1285 894 756 385 367 356 346 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2106 2194 2536 2603 3028 Size: 552 939 27 75 88 342 67 425 Cuts at: 3028 3433 513 Size: 405 5091 Fragments arranged by size: 5091 939 552 425 405 342 88 75 67 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7272 Fragments arranged by size: 7272 683 56 BtsI GCAGTG_nn' Cuts at: 2089 2324 2887 4782 5527 6321 6341 2089 Size: 235 563 1895 745 794 20 3759 Fragments arranged by size: 3759 1895 794 745 563 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8011 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4648 4683 529 Size: 35 4084 35 3857 Fragments arranged by size: 4084 3857 35 35 DraI TTT'AAA Cuts at: 4450 4501 4711 5315 4450 Size: 51 210 604 7146 Fragments arranged by size: 7146 604 210 51 DraIII CAC_nnn'GTG Cuts at: 2416 2416 Size: 8011 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7018 809 Size: 507 5702 1802 Fragments arranged by size: 5702 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2187 2191 Size: 54 1425 50 275 18 261 96 4 Cuts at: 2191 2568 2760 2830 2972 3816 3837 5458 6347 Size: 377 192 70 142 844 21 1621 889 Cuts at: 6347 7976 8 Size: 1629 43 Fragments arranged by size: 1629 1621 1425 889 844 377 275 261 192 142 96 70 54 50 43 21 18 4 EagI C'GGCC_G Cuts at: 1537 1812 5458 1537 Size: 275 3646 4090 Fragments arranged by size: 4090 3646 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2901 2961 5941 1353 Size: 1548 60 2980 3423 Fragments arranged by size: 3423 2980 1548 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2273 2576 6598 6972 7118 387 Size: 1064 138 684 303 4022 374 146 1280 Fragments arranged by size: 4022 1280 1064 684 374 303 146 138 EcoICRI GAG'CTC Cuts at: 2113 2113 Size: 8011 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2261 2543 2919 3281 3303 3968 Size: 393 401 228 282 376 362 22 665 Cuts at: 3968 4070 4776 6074 6671 7458 7726 1239 Size: 102 706 1298 597 787 268 1524 Fragments arranged by size: 1524 1298 787 706 665 597 401 393 376 362 282 268 228 102 22 EcoNI CCTnn'n_nnAGG Cuts at: 2951 4165 2951 Size: 1214 6797 EcoRI G'AATT_C Cuts at: 4330 5320 5698 4330 Size: 990 378 6643 Fragments arranged by size: 6643 990 378 EcoRV GAT'ATC Cuts at: 3112 3465 3112 Size: 353 7658 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3398 4259 4547 3398 Size: 861 288 6862 Fragments arranged by size: 6862 861 288 FseI GG_CCGG'CC Cuts at: 2193 2193 Size: 8011 FspI TGC'GCA Cuts at: 6517 6517 Size: 8011 HaeII r_GCGC'y Cuts at: 1678 2065 2177 2240 2474 2733 4032 4391 7158 Size: 387 112 63 234 259 1299 359 2767 Cuts at: 7158 1678 Size: 2531 Fragments arranged by size: 2767 2531 1299 387 359 259 234 112 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3429 3461 4238 4270 4562 Size: 32 135 32 1857 32 777 32 292 Cuts at: 4562 4594 6652 6684 6726 6758 1373 Size: 32 2058 32 42 32 2626 Fragments arranged by size: 2626 2058 1857 777 292 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3882 5130 5355 5601 669 Size: 988 281 1944 1248 225 246 3079 Fragments arranged by size: 3079 1944 1248 988 281 246 225 HindIII A'AGCT_T Cuts at: 748 3490 4313 4348 748 Size: 2742 823 35 4411 Fragments arranged by size: 4411 2742 823 35 HpaI GTT'AAC Cuts at: 3882 5130 5601 3882 Size: 1248 471 6292 Fragments arranged by size: 6292 1248 471 KasI G'GCGC_C Cuts at: 2061 2729 2061 Size: 668 7343 KpnI G_GTAC'C Cuts at: 5336 5336 Size: 8011 MfeI C'AATT_G Cuts at: 3665 3830 3989 4395 4485 4665 5610 3665 Size: 165 159 406 90 180 945 6066 Fragments arranged by size: 6066 945 406 180 165 159 90 MluI A'CGCG_T Cuts at: 7887 7887 Size: 8011 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7125 7309 1305 Size: 5820 184 2007 Fragments arranged by size: 5820 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 2762 3818 3839 10 Size: 54 1425 343 738 192 1056 21 4182 Fragments arranged by size: 4182 1425 1056 738 343 192 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2514 2756 3045 4184 4509 5969 6328 Size: 648 1348 242 289 1139 325 1460 359 Cuts at: 6328 6487 518 Size: 159 2042 Fragments arranged by size: 2042 1460 1348 1139 648 359 325 289 242 159 MspA1I CmG'CkG Cuts at: 1606 2422 2458 2977 5427 6104 7252 7497 1606 Size: 816 36 519 2450 677 1148 245 2120 Fragments arranged by size: 2450 2120 1148 816 677 519 245 36 NaeI GCC'GGC Cuts at: 2191 2191 Size: 8011 NarI GG'CG_CC Cuts at: 2062 2730 2062 Size: 668 7343 NcoI C'CATG_G Cuts at: 513 1065 2004 2106 513 Size: 552 939 102 6418 Fragments arranged by size: 6418 939 552 102 NdeI CA'TA_TG Cuts at: 387 4363 4603 387 Size: 3976 240 3795 Fragments arranged by size: 3976 3795 240 NgoMIV G'CCGG_C Cuts at: 2189 2189 Size: 8011 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8011 NotI GC'GGCC_GC Cuts at: 5458 5458 Size: 8011 NspI r_CATG'y Cuts at: 1132 1229 3457 4531 5369 6914 1132 Size: 97 2228 1074 838 1545 2229 Fragments arranged by size: 2229 2228 1545 1074 838 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8011 PciI A'CATG_T Cuts at: 3453 6910 3453 Size: 3457 4554 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 3835 1108 Size: 1167 1560 5284 Fragments arranged by size: 5284 1560 1167 PfoI T'CCnGG_A Cuts at: 2625 3894 4369 5157 2625 Size: 1269 475 788 5479 Fragments arranged by size: 5479 1269 788 475 PmeI GTTT'AAAC Cuts at: 5315 5315 Size: 8011 PmlI CAC'GTG Cuts at: 2185 2917 2185 Size: 732 7279 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2510 2542 3035 3067 6080 6112 7593 7625 2510 Size: 32 493 32 3013 32 1481 32 2896 Fragments arranged by size: 3013 2896 1481 493 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8011 PsiI TTA'TAA Cuts at: 5581 5581 Size: 8011 PstI C_TGCA'G Cuts at: 830 2742 3346 4329 5363 830 Size: 1912 604 983 1034 3478 Fragments arranged by size: 3478 1912 1034 983 604 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8011 SacI G_AGCT'C Cuts at: 2115 2115 Size: 8011 SalI G'TCGA_C Cuts at: 1936 5353 1936 Size: 3417 4594 SbfI CC_TGCA'GG Cuts at: 5363 5363 Size: 8011 ScaI AGT'ACT Cuts at: 6259 7971 6259 Size: 1712 6299 SexAI A'CCwGG_T Cuts at: 2343 2412 2844 2343 Size: 69 432 7510 Fragments arranged by size: 7510 432 69 SfcI C'TryA_G Cuts at: 826 1045 2738 3342 4325 4464 5359 6494 7175 Size: 219 1693 604 983 139 895 1135 681 Cuts at: 7175 7366 826 Size: 191 1471 Fragments arranged by size: 1693 1471 1135 983 895 681 604 219 191 139 SfoI GGC'GCC Cuts at: 2063 2731 2063 Size: 668 7343 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8011 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8011 SmaI CCC'GGG Cuts at: 1872 2400 5338 1872 Size: 528 2938 4545 Fragments arranged by size: 4545 2938 528 SmlI C'TyrA_G Cuts at: 820 1017 1943 2894 3245 3752 4099 4442 6115 Size: 197 926 951 351 507 347 343 1673 Cuts at: 6115 7016 7278 820 Size: 901 262 1553 Fragments arranged by size: 1673 1553 951 926 901 507 351 347 343 262 197 SnaBI TAC'GTA Cuts at: 493 5802 493 Size: 5309 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8011 SphI G_CATG'C Cuts at: 1132 5369 1132 Size: 4237 3774 SrfI GCCC'GGGC Cuts at: 2400 2400 Size: 8011 SspI AAT'ATT Cuts at: 5 52 4858 5935 5 Size: 47 4806 1077 2081 Fragments arranged by size: 4806 2081 1077 47 StuI AGG'CCT Cuts at: 1517 3338 1517 Size: 1821 6190 StyI C'CwwG_G Cuts at: 513 1065 2004 2106 3366 3484 5450 513 Size: 552 939 102 1260 118 1966 3074 Fragments arranged by size: 3074 1966 1260 939 552 118 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2596 2869 3774 6038 6055 6208 6393 Size: 1255 1016 273 905 2264 17 153 185 Cuts at: 6393 6838 325 Size: 445 1498 Fragments arranged by size: 2264 1498 1255 1016 905 445 273 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6257 7969 96 Size: 274 80 33 51 1443 4280 1712 138 Fragments arranged by size: 4280 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2417 2449 2621 2653 3215 3247 5232 5264 7971 Size: 32 172 32 562 32 1985 32 2707 Cuts at: 7971 8003 2417 Size: 32 2425 Fragments arranged by size: 2707 2425 1985 562 172 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8011 XbaI T'CTAG_A Cuts at: 5347 5347 Size: 8011 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8011 XmaI C'CCGG_G Cuts at: 1870 2398 5336 1870 Size: 528 2938 4545 Fragments arranged by size: 4545 2938 528 XmnI GAAnn'nnTTC Cuts at: 2910 4764 6140 2910 Size: 1854 1376 4781 Fragments arranged by size: 4781 1854 1376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2488 2497 2613 4279 Size: 53 83 186 936 954 9 116 1666 Cuts at: 4279 5819 276 Size: 1540 2468 Fragments arranged by size: 2468 1666 1540 954 936 186 116 83 53 9 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoO109I FspAI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SwaI XcmI