################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NarI NciI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AfaI 891 GTAC GT^AC AhdI 303 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 303 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BfaI 62 CTAG C^TAG BsaI 883 GAGACC GGTCTC(1/5) BsmBI 213 GAGACG CGTCTC(1/5) BspHI 626 TCATGA T^CATGA BspMI 341 ACCTGC ACCTGC(4/8) BsrBI 318 GAGCGG CCGCTC(-3/-3) BstF5I 789 GGATG GGATG(2/0) DrdI 793 GACGGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 303 GACAGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 315 GAAGAG CTCTTC(1/4) FokI 789 GGATG GGATG(9/13) FseI 4 GGCCGGCC GGCCGG^CC HgaI 554 GACGC GACGC(5/10) Ksp632I 315 GAAGAG CTCTTC(1/4) MaeI 62 CTAG C^TAG MaeII 197 ACGT A^CGT MboII 315 GAAGA GAAGA(8/7) MseI 901 TTAA T^TAA MspA1I 679 CAGCGG C[AC]G^C[GT]G NaeI 5 GCCGGC GCC^GGC NcoI 131 CCATGG C^CATGG NdeI 734 CATATG CA^TATG NgoMIV 5 GCCGGC G^CCGGC PleI 562 GACTC GAGTC(4/5) RcaI 626 TCATGA T^CATGA RsaI 891 GTAC GT^AC SacI 396 GAGCTC GAGCT^C SapI 315 GAAGAGC GCTCTTC(1/4) SfaNI 797 GCATC GCATC(5/9) SmlI 865 CTCAAG C^T[CT][GA]AG TaqI 425 TCGA T^CGA TfiI 623 GAATC G^A[AT]TC Tru9I 901 TTAA T^TAA TthHB8I 425 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwNI 74 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 602 CAGTGGCTG BanII 396 GAGCTC G[GA]GC[CT]^C 870 GAGCCC BsrI 299 ACTGG ACTGG(1/-1) 357 CCAGT Cac8I 5 GCCGGC GC[ACGT]^[ACGT]GC 45 GCCAGC DsaI 131 CCATGG C^C[GA][CT]GG 228 CCACGG EcoT14I 131 CCATGG C^C[AT][AT]GG 785 CCAAGG HinfI 562 GACTC G^A[ACGT]TC 623 GAATC HphI 446 TCACC GGTGA(8/7) 835 TCACC PstI 55 CTGCAG CTGCA^G 343 CTGCAG StyI 131 CCATGG C^C[AT][AT]GG 785 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI AlwI AocI AspHI AvaII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstNI BstYI Bsu36I Cfr10I Cfr13I DdeI DpnII Eco81I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MaeIII MboI MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfcI TseI Tsp45I TspRI XhoII