################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AccIII AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvrII BbeI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaBI BsaI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI BstAPI BstBI BstEII BstPI ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRI EcoRV FbaI FseI HaeII HincII HindII HindIII HpaI KasI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NspI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI PstI PvuI PvuII RcaI RsrII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 1141 GACGTC GACGT^C Acc65I 848 GGTACC G^GTACC AccI 325 GTATAC GT^[AC][GT]AC AccII 61 CGCG CG^CG AclI 217 AACGTT AA^CGTT AflIII 1241 ACACGT A^C[GA][CT]GT AlwNI 1033 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 848 GGTACC G^GTACC AviII 677 TGCGCA TGC^GCA BalI 257 TGGCCA TGG^CCA BamHI 607 GGATCC G^GATCC BanI 848 GGTACC G^G[CT][GA]CC BbrPI 1242 CACGTG CAC^GTG BsaAI 1242 CACGTG [CT]AC^GT[GA] BsaWI 101 ACCGGA [AT]^CCGG[AT] BseRI 1193 CTCCTC GAGGAG(10/8) BsiEI 75 CGGCCG CG[GA][CT]^CG BsmBI 125 GAGACG CGTCTC(1/5) Bsp1407I 363 TGTACA T^GTACA BsrFI 926 GCCGGC [GA]^CCGG[CT] BsrGI 363 TGTACA T^GTACA Bst1107I 325 GTATAC GTA^TAC BstUI 61 CGCG CG^CG BstXI 260 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 607 GGATCC [GA]^GATC[CT] BstZ17I 325 GTATAC GTA^TAC CfoI 678 GCGC GCG^C Cfr10I 926 GCCGGC [GA]^CCGG[CT] DrdI 1339 GACGCCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 75 CGGCCG C^GGCCG EclXI 75 CGGCCG C^GGCCG Eco52I 75 CGGCCG C^GGCCG EcoT22I 145 ATGCAT ATGCA^T FspI 677 TGCGCA TGC^GCA HgaI 1339 GACGC GACGC(5/10) HhaI 678 GCGC GCG^C HinP1I 678 GCGC G^CGC KpnI 848 GGTACC GGTAC^C KspI 60 CCGCGG CCGC^GG MflI 607 GGATCC [GA]^GATC[CT] MluNI 257 TGGCCA TGG^CCA MscI 257 TGGCCA TGG^CCA MvnI 61 CGCG CG^CG NaeI 926 GCCGGC GCC^GGC NgoMIV 926 GCCGGC G^CCGGC NsiI 145 ATGCAT ATGCA^T PaeR7I 1010 CTCGAG C^TCGAG PmaCI 1242 CACGTG CAC^GTG PmlI 1242 CACGTG CAC^GTG Psp1406I 217 AACGTT AA^CGTT SacI 91 GAGCTC GAGCT^C SacII 60 CCGCGG CCGC^GG SspBI 363 TGTACA T^GTACA StuI 1125 AGGCCT AGG^CCT XhoI 1010 CTCGAG C^TCGAG XhoII 607 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcsI 393 GAATTT [GA]^AATT[CT] 787 GAATTT AcyI 1141 GACGTC G[GA]^CG[CT]C 1339 GACGCC AocI 823 CCTCAGG CC^T[ACGT]AGG 1269 CCTCAGG ApaI 1150 GGGCCC GGGCC^C 1206 GGGCCC ApoI 393 GAATTT [GA]^AATT[CT] 787 GAATTT AspHI 91 GAGCTC G[AT]GC[AT]^C 1245 GTGCTC AvaI 1010 CTCGAG C^[CT]CG[GA]G 1097 CTCGGG BbvCI 444 CCTCAGC CCTCAGC(-5/-2) 387 GCTGAGG BfaI 238 CTAG C^TAG 662 CTAG BlpI 1038 GCTGAGC GC^T[ACGT]AGC 1060 GCTGAGC Bpu1102I 1038 GCTGAGC GC^T[ACGT]AGC 1060 GCTGAGC BsaHI 1141 GACGTC G[GA]^CG[CT]C 1339 GACGCC BsiHKAI 91 GAGCTC G[AT]GC[AT]^C 1245 GTGCTC BsoBI 1010 CTCGAG C^[CT]CG[GA]G 1097 CTCGGG BspMI 959 ACCTGC ACCTGC(4/8) 1091 ACCTGC BsrBI 40 CCGCTC CCGCTC(-3/-3) 877 CCGCTC BsrDI 311 GCAATG GCAATG(2/0) 1295 GCAATG BsrI 527 CCAGT ACTGG(1/-1) 1214 CCAGT Bsu36I 823 CCTCAGG CC^T[ACGT]AGG 1269 CCTCAGG CelII 1038 GCTGAGC GC^T[ACGT]AGC 1060 GCTGAGC DpnII 608 GATC ^GATC 1356 GATC Eco81I 823 CCTCAGG CC^T[ACGT]AGG 1269 CCTCAGG Hin1I 1141 GACGTC G[GA]^CG[CT]C 1339 GACGCC HphI 150 TCACC GGTGA(8/7) 874 TCACC MaeI 238 CTAG C^TAG 662 CTAG MaeIII 202 GTCAC ^GT[ACGT]AC 956 GTGAC MboI 608 GATC ^GATC 1356 GATC MslI 148 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 378 CACGGAGATG MspA1I 60 CCGCGG C[AC]G^C[GT]G 183 CAGCGG NcoI 153 CCATGG C^CATGG 1170 CCATGG NdeII 608 GATC ^GATC 1356 GATC PleI 130 GAGTC GAGTC(4/5) 793 GAGTC SapI 93 GCTCTTC GCTCTTC(1/4) 891 GCTCTTC Sau3AI 608 GATC ^GATC 1356 GATC SmlI 646 CTCAAG C^T[CT][GA]AG 1010 CTCGAG Tsp45I 202 GTCAC ^GT[GC]AC 956 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PpuMI RsaI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp509I TspRI TthHB8I