################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI BstXI CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 457 GTATAC GT^[AC][GT]AC AclI 446 AACGTT AA^CGTT AocI 883 CCTGAGG CC^T[ACGT]AGG Asp700I 904 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 92 CTCGAG C^[CT]CG[GA]G BalI 644 TGGCCA TGG^CCA BanII 1057 GAGCTC G[GA]GC[CT]^C BbvI 718 GCAGC GCAGC(8/12) BsaI 542 GAGACC GGTCTC(1/5) BsmI 84 GAATGC GAATGC(1/-1) BsoBI 92 CTCGAG C^[CT]CG[GA]G BssSI 10 CTCGTG CACGAG(-5/-1) Bst1107I 457 GTATAC GTA^TAC BstZ17I 457 GTATAC GTA^TAC Bsu36I 883 CCTGAGG CC^T[ACGT]AGG DraI 427 TTTAAA TTT^AAA DraII 887 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 1008 CCATGG C^C[GA][CT]GG EaeI 644 TGGCCA [CT]^GGCC[GA] Eco81I 883 CCTGAGG CC^T[ACGT]AGG EcoO109I 887 AGGACCC [GA]G^G[ACGT]CC[CT] Fnu4HI 718 GCAGC GC^[ACGT]GC HaeII 15 GGCGCT [GA]GCGC^[CT] HinfI 393 GAATC G^A[ACGT]TC ItaI 718 GCAGC GC^[ACGT]GC MaeII 447 ACGT A^CGT MluNI 644 TGGCCA TGG^CCA MscI 644 TGGCCA TGG^CCA NcoI 1008 CCATGG C^CATGG NspI 218 GCATGC [GA]CATG^[CT] PaeR7I 92 CTCGAG C^TCGAG PpuMI 887 AGGACCC [GA]G^G[AT]CC[CT] Psp1406I 446 AACGTT AA^CGTT SacI 1057 GAGCTC GAGCT^C ScaI 906 AGTACT AGT^ACT SfcI 618 CTACAG C^T[GA][CT]AG SmlI 92 CTCGAG C^T[CT][GA]AG SphI 218 GCATGC GCATG^C SwaI 426 ATTTAAAT ATTT^AAAT TfiI 393 GAATC G^A[AT]TC TseI 718 GCAGC G^C[AT]GC XhoI 92 CTCGAG C^TCGAG XmnI 904 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 430 AAATTT [GA]^AATT[CT] 1016 AAATTC ApoI 430 AAATTT [GA]^AATT[CT] 1016 AAATTC AspHI 948 GAGCAC G[AT]GC[AT]^C 1057 GAGCTC BfaI 176 CTAG C^TAG 298 CTAG BpmI 195 CTCCAG CTGGAG(16/14) 893 CTCCAG BsiHKAI 948 GAGCAC G[AT]GC[AT]^C 1057 GAGCTC BstYI 264 GGATCT [GA]^GATC[CT] 591 GGATCT Cac8I 183 GCTGGC GC[ACGT]^[ACGT]GC 218 GCATGC CfoI 16 GCGC GCG^C 232 GCGC EcoNI 129 CCTTATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 820 CCTGCCACAGG HaeIII 37 GGCC GG^CC 645 GGCC HhaI 16 GCGC GCG^C 232 GCGC HinP1I 16 GCGC G^CGC 232 GCGC MaeI 176 CTAG C^TAG 298 CTAG MflI 264 GGATCT [GA]^GATC[CT] 591 GGATCT MslI 792 CACCTTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 987 CACCTCTGTG SfaNI 845 GATGC GCATC(5/9) 914 GCATC XhoII 264 GGATCT [GA]^GATC[CT] 591 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI AlwI AvaII BanI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII EcoT14I FokI HphI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI StyI TaqI Tru9I Tsp509I TspRI TthHB8I