Restriction map for
pFN21AE9573
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspMI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MfeI MluI MluNI MscI
MseI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 1279 CGCG CG^CG
AccIII 297 TCCGGA T^CCGGA
AcyI 1256 GGCGCC G[GA]^CG[CT]C
ApaI 1217 GGGCCC GGGCC^C
AspHI 628 GAGCTC G[AT]GC[AT]^C
BbeI 1256 GGCGCC GGCGC^C
BbvCI 1338 GCTGAGG CCTCAGC(-5/-2)
BcgI 1368 CGAGGAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 487 CTAG C^TAG
BsaAI 1120 TACGTG [CT]AC^GT[GA]
BsaHI 1256 GGCGCC G[GA]^CG[CT]C
BsaI 1083 GAGACC GGTCTC(1/5)
BsaWI 297 TCCGGA [AT]^CCGG[AT]
BseAI 297 TCCGGA T^CCGGA
BseRI 1369 GAGGAG GAGGAG(10/8)
BsgI 547 CTGCAC GTGCAG(16/14)
BsiHKAI 628 GAGCTC G[AT]GC[AT]^C
BsmAI 1083 GAGAC GTCTC(1/5)
BsmFI 641 GGGAC GGGAC(10/14)
BspEI 297 TCCGGA T^CCGGA
BsrBI 1269 CCGCTC CCGCTC(-3/-3)
BsrDI 401 GCAATG GCAATG(2/0)
BssSI 631 CTCGTG CACGAG(-5/-1)
BstEII 540 GGTAACC G^GT[ACGT]ACC
BstPI 540 GGTAACC G^GT[ACGT]ACC
BstUI 1279 CGCG CG^CG
BstYI 722 GGATCT [GA]^GATC[CT]
DraII 641 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 903 CCATGG C^C[GA][CT]GG
EcoO109I 641 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 540 GGTAACC G^GT[ACGT]ACC
EcoRI 579 GAATTC G^AATTC
EcoT22I 431 ATGCAT ATGCA^T
Hin1I 1256 GGCGCC G[GA]^CG[CT]C
KasI 1256 GGCGCC G^GCGCC
MaeI 487 CTAG C^TAG
MflI 722 GGATCT [GA]^GATC[CT]
MroI 297 TCCGGA T^CCGGA
MvnI 1279 CGCG CG^CG
NarI 1256 GGCGCC GG^CGCC
NcoI 903 CCATGG C^CATGG
NdeI 434 CATATG CA^TATG
NsiI 431 ATGCAT ATGCA^T
PleI 1411 GAGTC GAGTC(4/5)
PpuMI 641 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1283 CTGCAG CTGCA^G
SacI 628 GAGCTC GAGCT^C
SfcI 1283 CTGCAG C^T[GA][CT]AG
SfoI 1256 GGCGCC GGC^GCC
TaqI 375 TCGA T^CGA
Tsp45I 970 GTGAC ^GT[GC]AC
TthHB8I 375 TCGA T^CGA
XhoII 722 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 579 GAATTC [GA]^AATT[CT]
1229 AAATTC
AflIII 557 ACATGT A^C[GA][CT]GT
1007 ACATGT
AlwNI 1286 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1333 CAGGAGCTG
ApoI 579 GAATTC [GA]^AATT[CT]
1229 AAATTC
BanI 813 GGTGCC G^G[CT][GA]CC
1256 GGCGCC
BcnI 645 CCCGG CC^[GC]GG
960 CCGGG
BpmI 260 CTGGAG CTGGAG(16/14)
1080 CTGGAG
BsaBI 301 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
588 GATTATCATC
BsmI 173 GAATGC GAATGC(1/-1)
429 GAATGC
BspLU11I 557 ACATGT A^CATGT
1007 ACATGT
BsrFI 20 GCCGGT [GA]^CCGG[CT]
255 ACCGGC
BsrI 678 CCAGT ACTGG(1/-1)
1062 CCAGT
BstAPI 768 GCAACGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1285 GCAGCCGCTGC
Cfr10I 20 GCCGGT [GA]^CCGG[CT]
255 ACCGGC
FauI 187 CCCGC CCCGC(4/6)
807 CCCGC
HaeII 1208 AGCGCC [GA]GCGC^[CT]
1256 GGCGCC
MaeII 122 ACGT A^CGT
1121 ACGT
MamI 301 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
588 GATTATCATC
MslI 483 CACCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
741 CATTGTCGTG
MspA1I 808 CCGCTG C[AC]G^C[GT]G
1289 CCGCTG
NciI 645 CCCGG CC^[GC]GG
960 CCGGG
NspI 557 ACATGT [GA]CATG^[CT]
1007 ACATGT
SexAI 379 ACCTGGT A^CC[AT]GGT
602 ACCTGGT
TspRI 32 CACTG CA[GC]TG[ACGT][ACGT]^
1234 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI TseI
Tsp509I