(Circular) (Six-base) MAPSORT of: pFN21AE9573.seq Check: 908 from: 1 to: 6127 pFN21AE9573 6127 bp With 182 enzymes: SgfI * September 11, 2018 19:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2450 3041 4190 5574 5842 1632 Size: 818 591 1149 1384 268 1917 Fragments arranged by size: 1917 1384 1149 818 591 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 2562 3012 5026 6003 1203 Size: 1359 450 2014 977 1327 Fragments arranged by size: 2014 1359 1327 977 450 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 2492 1166 Size: 1326 4801 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2684 1184 Size: 1500 4627 AlwNI CAG_nnn'CTG Cuts at: 3296 3343 3870 5442 3296 Size: 47 527 1572 3981 Fragments arranged by size: 3981 1572 527 47 ApaI G_GGCC'C Cuts at: 3226 3226 Size: 6127 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2584 3234 3436 3662 3814 5923 1493 Size: 1091 650 202 226 152 2109 1697 Fragments arranged by size: 2109 1697 1091 650 226 202 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3452 1870 Size: 73 1509 4545 Fragments arranged by size: 4545 1509 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2818 3261 3448 4903 618 Size: 325 217 627 1031 443 187 1455 1842 Fragments arranged by size: 1842 1455 1031 627 443 325 217 187 BanII G_rGCy'C Cuts at: 1391 2112 2637 3226 3446 1391 Size: 721 525 589 220 4072 Fragments arranged by size: 4072 721 589 525 220 BbeI G_GCGC'C Cuts at: 3265 3265 Size: 6127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5156 BbvCI CC'TCA_GC Cuts at: 3344 3344 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3362 3396 4318 4352 1903 Size: 34 1425 34 922 34 3678 Fragments arranged by size: 3678 1425 922 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BfrBI ATG'CAT Cuts at: 2438 2438 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 3054 3226 4188 5344 1790 Size: 1264 172 962 1156 2573 Fragments arranged by size: 2573 1264 1156 962 172 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 3106 4787 1239 Size: 1047 820 1681 2579 Fragments arranged by size: 2579 1681 1047 820 Bpu10I CC'TnA_GC Cuts at: 3344 3344 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4252 5117 5415 4252 Size: 865 298 4964 Fragments arranged by size: 4964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3082 4790 882 Size: 617 1583 1708 2219 Fragments arranged by size: 2219 1708 1583 617 BsaAI yAC'GTr Cuts at: 493 1204 3127 3918 493 Size: 711 1923 791 2702 Fragments arranged by size: 2702 1923 791 711 BsaBI GATnn'nnATC Cuts at: 2310 2597 6121 2310 Size: 287 3524 2316 Fragments arranged by size: 3524 2316 287 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3262 3934 4316 4951 Size: 53 83 186 936 1729 672 382 635 Cuts at: 4951 275 Size: 1451 Fragments arranged by size: 1729 1451 936 672 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2302 4560 4958 5232 5379 5790 2302 Size: 2258 398 274 147 411 2639 Fragments arranged by size: 2639 2258 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2622 2652 1178 Size: 30 165 30 1219 30 4653 Fragments arranged by size: 4653 1219 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3389 1186 Size: 2203 3924 BseYI C'CCAG_C Cuts at: 2247 2802 3058 5330 5826 2247 Size: 555 256 2272 496 2548 Fragments arranged by size: 2548 2272 555 496 256 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 1163 Size: 573 801 4753 Fragments arranged by size: 4753 801 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2637 4188 4273 5344 2637 Size: 1551 85 1071 3420 Fragments arranged by size: 3420 1551 1071 85 BsmI GAATG_Cn' Cuts at: 2184 2440 3638 3731 2184 Size: 256 1198 93 4580 Fragments arranged by size: 4580 1198 256 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2637 3054 3226 3446 4188 4273 Size: 399 322 525 417 172 220 742 85 Cuts at: 4273 5344 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 525 417 399 322 220 172 85 BspEI T'CCGG_A Cuts at: 2302 5790 2302 Size: 3488 2639 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3276 4014 1394 Size: 429 1453 738 3507 Fragments arranged by size: 3507 1453 738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2413 4622 4796 65 Size: 1183 1165 2209 174 1396 Fragments arranged by size: 2209 1396 1183 1165 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2025 2260 4771 4958 1700 Size: 110 215 235 2511 187 2869 Fragments arranged by size: 2869 2511 235 215 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 2636 4187 5199 2636 Size: 1551 1012 3564 Fragments arranged by size: 3564 1551 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2779 3296 794 Size: 1985 517 3625 Fragments arranged by size: 3625 1985 517 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 2545 1184 Size: 1361 4766 BstYI r'GATC_y Cuts at: 1972 2727 3457 3824 4209 4226 5678 5764 5776 Size: 755 730 367 385 17 1452 86 12 Cuts at: 5776 6122 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 755 730 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2908 513 Size: 552 1843 3732 Fragments arranged by size: 3732 1843 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 3643 4437 4457 3643 Size: 794 20 5313 Fragments arranged by size: 5313 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092 Size: 54 1425 50 275 18 1744 889 1629 Cuts at: 6092 8 Size: 43 Fragments arranged by size: 1744 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4057 1353 Size: 2704 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3017 4714 5088 5234 387 Size: 1064 138 1428 1697 374 146 1280 Fragments arranged by size: 1697 1428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2635 2635 Size: 6127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2450 3041 3106 4190 4787 5574 Size: 393 654 164 591 65 1084 597 787 Cuts at: 5574 5842 1239 Size: 268 1524 Fragments arranged by size: 1524 1084 787 654 597 591 393 268 164 65 EcoO109I rG'GnC_Cy Cuts at: 2647 2647 Size: 6127 EcoRI G'AATT_C Cuts at: 2584 3436 3814 2584 Size: 852 378 4897 Fragments arranged by size: 4897 852 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2794 2794 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 3217 3265 5274 1678 Size: 1539 48 2009 2531 Fragments arranged by size: 2531 2009 1539 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2990 3022 3121 3153 3401 Size: 32 135 32 1418 32 99 32 248 Cuts at: 3401 3433 4768 4800 4842 4874 1373 Size: 32 1335 32 42 32 2626 Fragments arranged by size: 2626 1418 1335 248 135 99 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KasI G'GCGC_C Cuts at: 3261 3261 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2123 2415 3120 3294 5241 5425 1305 Size: 818 292 705 174 1947 184 2007 Fragments arranged by size: 2007 1947 818 705 292 184 174 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 2750 4085 4444 4603 518 Size: 648 1326 258 1335 359 159 2042 Fragments arranged by size: 2042 1335 1326 648 359 258 159 MspA1I CmG'CkG Cuts at: 1606 2815 3296 3543 4220 5368 5613 1606 Size: 1209 481 247 677 1148 245 2120 Fragments arranged by size: 2120 1209 1148 677 481 247 245 NarI GG'CG_CC Cuts at: 3262 3262 Size: 6127 NcoI C'CATG_G Cuts at: 513 1065 2908 513 Size: 552 1843 3732 Fragments arranged by size: 3732 1843 552 NdeI CA'TA_TG Cuts at: 387 2440 387 Size: 2053 4074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NsiI A_TGCA'T Cuts at: 2440 2440 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2566 3016 3485 5030 1132 Size: 97 1337 450 469 1545 2229 Fragments arranged by size: 2229 1545 1337 469 450 97 PasI CC'CwG_GG Cuts at: 1348 2503 1348 Size: 1155 4972 PciI A'CATG_T Cuts at: 2562 3012 5026 2562 Size: 450 2014 3663 Fragments arranged by size: 3663 2014 450 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2647 2647 Size: 6127 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 3222 3222 Size: 6127 PstI C_TGCA'G Cuts at: 830 3292 3479 830 Size: 2462 187 3478 Fragments arranged by size: 3478 2462 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 SacI G_AGCT'C Cuts at: 2637 2637 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SanDI GG'GwC_CC Cuts at: 2647 2647 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SexAI A'CCwGG_T Cuts at: 2384 2607 2384 Size: 223 5904 SfcI C'TryA_G Cuts at: 826 1045 3288 3475 4610 5291 5482 826 Size: 219 2243 187 1135 681 191 1471 Fragments arranged by size: 2243 1471 1135 681 219 191 187 SfoI GGC'GCC Cuts at: 3263 3263 Size: 6127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4231 5132 5394 820 Size: 197 926 2288 901 262 1553 Fragments arranged by size: 2288 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2200 2530 2908 3367 3566 513 Size: 552 1135 330 378 459 199 3074 Fragments arranged by size: 3074 1135 552 459 378 330 199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2073 2190 4154 4171 4324 4509 4954 Size: 1255 493 117 1964 17 153 185 445 Cuts at: 4954 325 Size: 1498 Fragments arranged by size: 1964 1498 1255 493 445 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 2448 2685 Size: 274 80 33 51 1443 183 288 237 Cuts at: 2685 2853 4373 6085 96 Size: 168 1520 1712 138 Fragments arranged by size: 1712 1520 1443 288 274 237 183 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2654 2704 2736 6087 6119 2622 Size: 32 50 32 3351 32 2630 Fragments arranged by size: 3351 2630 50 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI XcmI