################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BstEII BstPI BstUI Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FauI FseI FspI HincII HindII HpaI KasI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1786 GACGTC GACGT^C Acc65I 2775 GGTACC G^GTACC AcyI 1786 GACGTC G[GA]^CG[CT]C AflIII 625 ACACGT A^C[GA][CT]GT Alw44I 493 GTGCAC G^TGCAC ApaI 1583 GGGCCC GGGCC^C ApaLI 493 GTGCAC G^TGCAC Asp718I 2775 GGTACC G^GTACC BciVI 3618 GGATAC GTATCC(6/5) BclI 3029 TGATCA T^GATCA BcnI 3411 CCGGG CC^[GC]GG BsaHI 1786 GACGTC G[GA]^CG[CT]C BsgI 1249 GTGCAG GTGCAG(16/14) BsiEI 3139 CGACCG CG[GA][CT]^CG BsmI 3714 GAATGC GAATGC(1/-1) BsrBI 3250 GAGCGG CCGCTC(-3/-3) BssSI 3560 CTCGTG CACGAG(-5/-1) Bst1107I 2072 GTATAC GTA^TAC BstAPI 922 GCATCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1134 TTCGAA TT^CGAA BstZ17I 2072 GTATAC GTA^TAC DraI 49 TTTAAA TTT^AAA EcoNI 2087 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1177 GAATTC G^AATTC EcoT22I 920 ATGCAT ATGCA^T FbaI 3029 TGATCA T^GATCA HgaI 3192 GACGC GACGC(5/10) Hin1I 1786 GACGTC G[GA]^CG[CT]C HindIII 2041 AAGCTT A^AGCTT KpnI 2775 GGTACC GGTAC^C MfeI 2588 CAATTG C^AATTG MunI 2588 CAATTG C^AATTG NciI 3411 CCGGG CC^[GC]GG NdeI 2752 CATATG CA^TATG NsiI 920 ATGCAT ATGCA^T NspI 3462 ACATGC [GA]CATG^[CT] NspV 1134 TTCGAA TT^CGAA PstI 3372 CTGCAG CTGCA^G ScaI 990 AGTACT AGT^ACT SexAI 334 ACCAGGT A^CC[AT]GGT SfuI 1134 TTCGAA TT^CGAA SmlI 2468 CTCAAG C^T[CT][GA]AG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 1767 GTCTAC GT^[AC][GT]AC 2072 GTATAC AseI 544 ATTAAT AT^TAAT 1014 ATTAAT AsnI 544 ATTAAT AT^TAAT 1014 ATTAAT Asp700I 1456 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2288 GAATTATTTC BalI 656 TGGCCA TGG^CCA 3534 TGGCCA BanII 1583 GGGCCC G[GA]GC[CT]^C 3355 GAGCCC BbrPI 626 CACGTG CAC^GTG 1625 CACGTG BfaI 2278 CTAG C^TAG 2668 CTAG BglII 258 AGATCT A^GATCT 1223 AGATCT BsaAI 626 CACGTG [CT]AC^GT[GA] 1625 CACGTG BsaWI 2870 TCCGGT [AT]^CCGG[AT] 3141 ACCGGA BseRI 26 GAGGAG GAGGAG(10/8) 1076 GAGGAG BspMI 3676 ACCTGC ACCTGC(4/8) 2772 GCAGGT CfoI 2675 GCGC GCG^C 3834 GCGC DraII 1232 AGGGCCT [GA]G^G[ACGT]CC[CT] 1582 GGGGCCC EcoO109I 1232 AGGGCCT [GA]G^G[ACGT]CC[CT] 1582 GGGGCCC EcoRV 229 GATATC GAT^ATC 289 GATATC HaeII 2674 GGCGCT [GA]GCGC^[CT] 3833 AGCGCC HhaI 2675 GCGC GCG^C 3834 GCGC HinP1I 2675 GCGC G^CGC 3834 GCGC MaeI 2278 CTAG C^TAG 2668 CTAG MluNI 656 TGGCCA TGG^CCA 3534 TGGCCA MscI 656 TGGCCA TGG^CCA 3534 TGGCCA PflMI 3189 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3240 CCAGCTCCTGG PmaCI 626 CACGTG CAC^GTG 1625 CACGTG PmlI 626 CACGTG CAC^GTG 1625 CACGTG PshBI 544 ATTAAT AT^TAAT 1014 ATTAAT SapI 1296 GAAGAGC GCTCTTC(1/4) 3225 GAAGAGC StuI 2166 AGGCCT AGG^CCT 3072 AGGCCT Van91I 3189 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3240 CCAGCTCCTGG XmnI 1456 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2288 GAATTATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BbsI BbvI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII