################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MslI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfaNI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 742 AAATTT [GA]^AATT[CT] Aor51HI 74 AGCGCT AGC^GCT ApoI 742 AAATTT [GA]^AATT[CT] Asp700I 72 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 506 GTGCTC G[AT]GC[AT]^C AvaI 639 CCCGGG C^[CT]CG[GA]G BalI 606 TGGCCA TGG^CCA BbvI 644 GCTGC GCAGC(8/12) BclI 578 TGATCA T^GATCA BglII 207 AGATCT A^GATCT BmyI 506 GTGCTC G[AGT]GC[ACT]^C BpmI 310 CTGGAG CTGGAG(16/14) BsaI 375 GAGACC GGTCTC(1/5) BsiHKAI 506 GTGCTC G[AT]GC[AT]^C BsmAI 375 GAGAC GTCTC(1/5) BsmFI 326 GGGAC GGGAC(10/14) BsoBI 639 CCCGGG C^[CT]CG[GA]G Bsp1286I 506 GTGCTC G[AGT]GC[ACT]^C BsrBI 120 GAGCGG CCGCTC(-3/-3) BsrDI 330 CATTGC GCAATG(2/0) BstBI 79 TTCGAA TT^CGAA CfoI 75 GCGC GCG^C DdeI 162 CTCAG C^T[ACGT]AG DraIII 160 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 47 CCGTGG C^C[GA][CT]GG Eco47III 74 AGCGCT AGC^GCT FbaI 578 TGATCA T^GATCA HaeII 74 AGCGCT [GA]GCGC^[CT] HhaI 75 GCGC GCG^C HinP1I 75 GCGC G^CGC HinfI 360 GAATC G^A[ACGT]TC MaeII 504 ACGT A^CGT MluNI 606 TGGCCA TGG^CCA MscI 606 TGGCCA TGG^CCA MseI 481 TTAA T^TAA MwoI 113 GCTGGCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspV 79 TTCGAA TT^CGAA PstI 151 CTGCAG CTGCA^G ScaI 705 AGTACT AGT^ACT SfcI 151 CTGCAG C^T[GA][CT]AG SfuI 79 TTCGAA TT^CGAA SmaI 639 CCCGGG CCC^GGG SmlI 568 CTCAAG C^T[CT][GA]AG TfiI 360 GAATC G^A[AT]TC Tru9I 481 TTAA T^TAA TseI 644 GCTGC G^C[AT]GC XcmI 38 CCAATGTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 639 CCCGGG C^CCGGG XmaI 639 CCCGGG C^CCGGG XmnI 72 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 27 GATCC GGATC(4/5) 390 GATCC BsaJI 47 CCGTGG C^C[ACGT][ACGT]GG 639 CCCGGG BsiYI 47 CCGTGGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 420 CCACTATTCGG BslI 47 CCGTGGTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 420 CCACTATTCGG BsrI 309 ACTGG ACTGG(1/-1) 535 CCAGT BstYI 26 AGATCC [GA]^GATC[CT] 207 AGATCT EaeI 44 TGGCCG [CT]^GGCC[GA] 606 TGGCCA Fnu4HI 122 GCGGC GC^[ACGT]GC 644 GCTGC HaeIII 45 GGCC GG^CC 607 GGCC HapII 68 CCGG C^CGG 640 CCGG HpaII 68 CCGG C^CGG 640 CCGG HphI 429 GGTGA GGTGA(8/7) 11 TCACC ItaI 122 GCGGC GC^[ACGT]GC 644 GCTGC MflI 26 AGATCC [GA]^GATC[CT] 207 AGATCT MnlI 138 CCTC CCTC(7/6) 516 GAGG MspI 68 CCGG C^CGG 640 CCGG NlaIII 408 CATG CATG^ 513 CATG Tsp509I 657 AATT ^AATT 743 AATT XhoII 26 AGATCC [GA]^GATC[CT] 207 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AciI AfaI AluI BcnI BssKI BstF5I Cac8I DpnII FokI MboI MboII NciI NdeII RsaI Sau3AI ScrFI TaqI TspRI TthHB8I