################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TspRI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AciI 219 GCGG CCGC(-3/-1) AcsI 103 GAATTT [GA]^AATT[CT] AlwI 189 GATCC GGATC(4/5) ApoI 103 GAATTT [GA]^AATT[CT] Asp700I 241 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 336 GGACC G^G[AT]CC BanI 77 GGCACC G^G[CT][GA]CC BbvI 156 GCTGC GCAGC(8/12) BlpI 289 GCTGAGC GC^T[ACGT]AGC BpmI 151 CTCCAG CTGGAG(16/14) Bpu1102I 289 GCTGAGC GC^T[ACGT]AGC BseRI 229 GAGGAG GAGGAG(10/8) BsiYI 192 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 192 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 43 GAGAC GTCTC(1/5) BsmBI 43 GAGACG CGTCTC(1/5) BsmI 266 GCATTC GAATGC(1/-1) BsrBI 217 GAGCGG CCGCTC(-3/-3) BsrFI 167 ACCGGC [GA]^CCGG[CT] BstF5I 12 GGATG GGATG(2/0) BstYI 188 AGATCC [GA]^GATC[CT] CelII 289 GCTGAGC GC^T[ACGT]AGC CfoI 25 GCGC GCG^C Cfr10I 167 ACCGGC [GA]^CCGG[CT] Cfr13I 336 GGACC G^G[ACGT]CC DpnII 189 GATC ^GATC DraII 335 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 335 AGGACCC [GA]G^G[ACGT]CC[CT] FokI 12 GGATG GGATG(9/13) HaeIII 170 GGCC GG^CC HapII 168 CCGG C^CGG HhaI 25 GCGC GCG^C HinP1I 25 GCGC G^CGC HincII 132 GTTGAC GT[CT]^[GA]AC HindII 132 GTTGAC GT[CT]^[GA]AC HinfI 84 GAGTC G^A[ACGT]TC HpaII 168 CCGG C^CGG HphI 260 TCACC GGTGA(8/7) MaeII 46 ACGT A^CGT MboI 189 GATC ^GATC MflI 188 AGATCC [GA]^GATC[CT] MseI 49 TTAA T^TAA MspI 168 CCGG C^CGG NdeII 189 GATC ^GATC NlaIII 71 CATG CATG^ PflMI 192 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 84 GAGTC GAGTC(4/5) PpuMI 335 AGGACCC [GA]G^G[AT]CC[CT] PstI 275 CTGCAG CTGCA^G Sau3AI 189 GATC ^GATC Sau96I 336 GGACC G^G[ACGT]CC SfaNI 13 GATGC GCATC(5/9) SfcI 275 CTGCAG C^T[GA][CT]AG Tru9I 49 TTAA T^TAA TseI 156 GCTGC G^C[AT]GC Van91I 192 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 188 AGATCC [GA]^GATC[CT] XmnI 241 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwNI 154 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 284 CAGAGGCTG BsrDI 314 GCAATG GCAATG(2/0) 322 GCAATG Cac8I 74 GCAGGC GC[ACGT]^[ACGT]GC 273 GCCTGC Fnu4HI 156 GCTGC GC^[ACGT]GC 219 GCGGC ItaI 156 GCTGC GC^[ACGT]GC 219 GCGGC MspA1I 154 CAGCTG C[AC]G^C[GT]G 193 CAGCTG NlaIV 77 GGCACC GG[ACGT]^[ACGT]CC 336 GGACCC PvuII 154 CAGCTG CAG^CTG 193 CAGCTG SapI 214 GAAGAGC GCTCTTC(1/4) 244 GCTCTTC Tsp45I 175 GTGAC ^GT[GC]AC 259 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### AluI DdeI EarI Ksp632I MaeIII MboII MnlI MwoI Tsp509I