(Circular) (Six-base) MAPSORT of: pFN21AE9446.seq Check: 9929 from: 1 to: 5836 pFN21AE9446 5836 bp With 182 enzymes: SgfI * March 28, 2012 12:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2457 3179 1937 Size: 520 722 4594 Fragments arranged by size: 4594 722 520 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2986 3899 5283 5551 1632 Size: 492 862 913 1384 268 1917 Fragments arranged by size: 1917 1384 913 862 492 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2059 2956 3579 5151 2059 Size: 897 623 1572 2744 Fragments arranged by size: 2744 1572 897 623 ApaI G_GGCC'C Cuts at: 2602 2602 Size: 5836 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 3145 3371 3523 5632 1493 Size: 1652 226 152 2109 1697 Fragments arranged by size: 2109 1697 1652 226 152 AseI AT'TA_AT Cuts at: 160 1032 2322 4390 160 Size: 872 1290 2068 1606 Fragments arranged by size: 2068 1606 1290 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 3161 1870 Size: 73 1218 4545 Fragments arranged by size: 4545 1218 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 3157 4612 618 Size: 325 217 627 563 807 1455 1842 Fragments arranged by size: 1842 1455 807 627 563 325 217 BanII G_rGCy'C Cuts at: 1391 2602 3127 3155 1391 Size: 1211 525 28 4072 Fragments arranged by size: 4072 1211 525 28 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2992 928 Size: 971 1093 3772 Fragments arranged by size: 3772 1093 971 BbvCI CC'TCA_GC Cuts at: 2429 2429 Size: 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4027 4061 1903 Size: 34 50 34 2006 34 3678 Fragments arranged by size: 3678 2006 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 2550 2943 1548 Size: 1002 393 4441 Fragments arranged by size: 4441 1002 393 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2602 3897 5053 1790 Size: 812 1295 1156 2573 Fragments arranged by size: 2573 1295 1156 812 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2066 3567 4525 Size: 416 53 83 399 24 649 1501 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1501 958 649 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2358 2536 3035 4496 1239 Size: 1119 178 499 1461 2579 Fragments arranged by size: 2579 1461 1119 499 178 Bpu10I CC'TnA_GC Cuts at: 2429 2429 Size: 5836 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4499 882 Size: 617 3000 2219 Fragments arranged by size: 3000 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5611 Fragments arranged by size: 5611 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2245 1186 Size: 1059 4777 BseYI C'CCAG_C Cuts at: 2595 5039 5535 2595 Size: 2444 496 2896 Fragments arranged by size: 2896 2444 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2609 2794 1163 Size: 573 873 185 4205 Fragments arranged by size: 4205 873 573 185 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3897 3982 5053 3897 Size: 85 1071 4680 Fragments arranged by size: 4680 1071 85 BsmI GAATG_Cn' Cuts at: 3347 3440 3347 Size: 93 5743 BsmBI CGTCTCn'nnnn_ Cuts at: 3044 3044 Size: 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2602 3127 3155 3897 3982 5053 1391 Size: 399 812 525 28 742 85 1071 2174 Fragments arranged by size: 2174 1071 812 742 525 399 85 28 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2377 2964 3191 844 Size: 1533 587 227 3489 Fragments arranged by size: 3489 1533 587 227 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2392 794 Size: 1598 4238 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstYI r'GATC_y Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831 Size: 1194 367 385 17 1452 86 12 346 Cuts at: 5831 1972 Size: 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5284 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2597 3352 4146 4166 2597 Size: 755 794 20 4267 Fragments arranged by size: 4267 794 755 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 3104 3283 Size: 54 1425 50 275 18 444 830 179 Cuts at: 3283 4172 5801 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 830 444 275 179 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2558 3132 3766 1353 Size: 1205 574 634 3423 Fragments arranged by size: 3423 1205 634 574 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4423 4797 4943 387 Size: 1064 138 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2358 2536 2986 3035 3899 4496 Size: 393 492 234 178 450 49 864 597 Cuts at: 4496 5283 5551 1239 Size: 787 268 1524 Fragments arranged by size: 1524 864 787 597 492 450 393 268 234 178 49 EcoO109I rG'GnC_Cy Cuts at: 2176 2497 2599 2176 Size: 321 102 5413 Fragments arranged by size: 5413 321 102 EcoRI G'AATT_C Cuts at: 3145 3523 3145 Size: 378 5458 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 4983 1678 Size: 3305 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2781 2813 4477 4509 4551 Size: 32 135 32 1209 32 1664 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 1664 1209 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2305 3180 3426 669 Size: 988 281 367 875 246 3079 Fragments arranged by size: 3079 988 875 367 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 2159 3435 2159 Size: 1276 4560 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2179 2601 2775 2843 4950 5134 1305 Size: 874 422 174 68 2107 184 2007 Fragments arranged by size: 2107 2007 874 422 184 174 68 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 3106 10 Size: 54 1425 343 444 830 2740 Fragments arranged by size: 2740 1425 830 444 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3794 4153 4312 518 Size: 648 2628 359 159 2042 Fragments arranged by size: 2628 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2272 3252 3929 5077 5322 1606 Size: 666 980 677 1148 245 2120 Fragments arranged by size: 2120 1148 980 677 666 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PfoI T'CCnGG_A Cuts at: 3051 3051 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2176 2497 2176 Size: 321 5515 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2598 2598 Size: 5836 PstI C_TGCA'G Cuts at: 830 3029 3188 830 Size: 2199 159 3478 Fragments arranged by size: 3478 2199 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2272 2272 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 3132 3132 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3029 3188 3029 Size: 159 5677 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SexAI A'CCwGG_T Cuts at: 2358 2358 Size: 5836 SfcI C'TryA_G Cuts at: 826 1045 3025 3184 4319 5000 5191 826 Size: 219 1980 159 1135 681 191 1471 Fragments arranged by size: 1980 1471 1135 681 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 3131 3275 513 Size: 552 2066 144 3074 Fragments arranged by size: 3074 2066 552 144 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2933 3863 3880 4033 4218 4663 325 Size: 1255 1353 930 17 153 185 445 1498 Fragments arranged by size: 1498 1353 1255 930 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2658 4082 5794 Size: 274 80 33 51 1443 681 1424 1712 Cuts at: 5794 96 Size: 138 Fragments arranged by size: 1712 1443 1424 681 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 2572 1316 Size: 1256 4580 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI