(Circular) (Six-base) MAPSORT of: pFN21AE3414.seq Check: 7194 from: 1 to: 5764 pFN21AE3414 5764 bp With 182 enzymes: SgfI * October 20, 2017 17:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3827 5211 5479 1632 Size: 2195 1384 268 1917 Fragments arranged by size: 2195 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2086 820 Size: 197 1069 4498 Fragments arranged by size: 4498 1069 197 AflIII A'CryG_T Cuts at: 1203 4663 5640 1203 Size: 3460 977 1327 Fragments arranged by size: 3460 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 3507 5079 3507 Size: 1572 4192 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2656 3073 3299 3451 5560 1493 Size: 1163 417 226 152 2109 1697 Fragments arranged by size: 2109 1697 1163 417 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3089 1870 Size: 73 1146 4545 Fragments arranged by size: 4545 1146 73 BamHI G'GATC_C Cuts at: 2167 3094 3461 2167 Size: 927 367 4470 Fragments arranged by size: 4470 927 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 4540 618 Size: 325 217 627 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 627 325 217 BanII G_rGCy'C Cuts at: 1391 2326 3083 1391 Size: 935 757 4072 Fragments arranged by size: 4072 935 757 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4793 BbvCI CC'TCA_GC Cuts at: 2223 2223 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 3825 4981 1790 Size: 2035 1156 2573 Fragments arranged by size: 2573 2035 1156 BmgBI CAC'GTC Cuts at: 1318 2717 1318 Size: 1399 4365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2778 4424 1239 Size: 1539 1646 2579 Fragments arranged by size: 2579 1646 1539 Bpu10I CC'TnA_GC Cuts at: 2223 2223 Size: 5764 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3889 4754 5052 3889 Size: 865 298 4601 Fragments arranged by size: 4601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2642 3555 493 Size: 711 1438 913 2702 Fragments arranged by size: 2702 1438 913 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3571 3953 4588 275 Size: 53 83 186 936 2038 382 635 1451 Fragments arranged by size: 2038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5539 Fragments arranged by size: 5539 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2628 2703 2763 2808 2817 2892 3067 1186 Size: 1442 75 60 45 9 75 175 3883 Fragments arranged by size: 3883 1442 175 75 75 60 45 9 BseYI C'CCAG_C Cuts at: 4967 5463 4967 Size: 496 5268 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3825 3910 4981 3825 Size: 85 1071 4608 Fragments arranged by size: 4608 1071 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 3083 3825 3910 4981 1391 Size: 399 536 757 742 85 1071 2174 Fragments arranged by size: 2174 1071 757 742 536 399 85 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3119 844 Size: 2275 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 3824 4836 3824 Size: 1012 4752 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstYI r'GATC_y Cuts at: 1972 2167 2693 3094 3461 3846 3863 5315 5401 Size: 195 526 401 367 385 17 1452 86 Cuts at: 5401 5413 5759 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 526 401 385 367 346 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2217 2238 513 Size: 552 939 213 21 4039 Fragments arranged by size: 4039 939 552 213 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2716 3031 3280 4074 4094 2716 Size: 315 249 794 20 4386 Fragments arranged by size: 4386 794 315 249 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2824 2859 529 Size: 35 2260 35 3434 Fragments arranged by size: 3434 2260 35 35 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2645 2645 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729 Size: 54 1425 50 275 18 1381 889 1629 Cuts at: 5729 8 Size: 43 Fragments arranged by size: 1629 1425 1381 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2605 2794 2948 3694 1353 Size: 1252 189 154 746 3423 Fragments arranged by size: 3423 1252 746 189 154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2370 4351 4725 4871 387 Size: 1064 138 781 1981 374 146 1280 Fragments arranged by size: 1981 1280 1064 781 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2778 3827 4424 5211 5479 1239 Size: 393 1146 1049 597 787 268 1524 Fragments arranged by size: 1524 1146 1049 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2753 2822 2753 Size: 69 5695 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2580 2580 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 4911 1678 Size: 3233 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2432 2464 4405 4437 4479 Size: 32 135 32 860 32 1941 32 42 Cuts at: 4479 4511 1373 Size: 32 2626 Fragments arranged by size: 2626 1941 860 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 2089 2141 748 Size: 1341 52 4371 Fragments arranged by size: 4371 1341 52 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 2259 3363 2259 Size: 1104 4660 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3722 4081 4240 518 Size: 648 2556 359 159 2042 Fragments arranged by size: 2556 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3180 3857 5005 5250 1606 Size: 1574 677 1148 245 2120 Fragments arranged by size: 2120 1574 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2217 2238 513 Size: 552 939 213 21 4039 Fragments arranged by size: 4039 939 552 213 21 NdeI CA'TA_TG Cuts at: 387 2995 387 Size: 2608 3156 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2999 1108 Size: 1891 3873 PfoI T'CCnGG_A Cuts at: 3016 3016 Size: 5764 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PmlI CAC'GTG Cuts at: 2642 2642 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2753 2822 2753 Size: 69 5695 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 2922 3334 2922 Size: 412 5352 PstI C_TGCA'G Cuts at: 830 3116 830 Size: 2286 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SfcI C'TryA_G Cuts at: 826 1045 2292 3112 4247 4928 5119 826 Size: 219 1247 820 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 820 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2086 3868 4769 5031 820 Size: 197 926 143 1782 901 262 1553 Fragments arranged by size: 1782 1553 926 901 262 197 143 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5764 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 2238 3203 513 Size: 552 939 213 21 965 3074 Fragments arranged by size: 3074 965 939 552 213 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5724 5756 5724 Size: 32 5732 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 2570 3100 2570 Size: 530 5234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2534 2534 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 2025 3893 2025 Size: 1868 3896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI