(Linear) (Six-base) MAPSORT of: KIEE6756.seq Check: 4931 from: 1 to: 2436 KIEE6756.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 707 1319 1360 2198 2436 Size: 707 612 41 838 238 Fragments arranged by size: 838 707 612 238 41 AflII C'TTAA_G Cuts at: 0 1273 2436 Size: 1273 1163 AflIII A'CryG_T Cuts at: 0 1693 2436 Size: 1693 743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1646 2436 Size: 1646 790 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 765 797 2436 Size: 765 32 1639 Fragments arranged by size: 1639 765 32 AlwNI CAG_nnn'CTG Cuts at: 0 1823 2436 Size: 1823 613 ApoI r'AATT_y Cuts at: 0 202 704 863 950 1076 1704 2189 2436 Size: 202 502 159 87 126 628 485 247 Fragments arranged by size: 628 502 485 247 202 159 126 87 BanI G'GyrC_C Cuts at: 0 2022 2436 Size: 2022 414 BanII G_rGCy'C Cuts at: 0 234 2172 2384 2436 Size: 234 1938 212 52 Fragments arranged by size: 1938 234 212 52 BbsI GAAGACnn'nnnn_ Cuts at: 0 673 2436 Size: 673 1763 BbvCI CC'TCA_GC Cuts at: 0 650 2436 Size: 650 1786 BfrBI ATG'CAT Cuts at: 0 1432 2436 Size: 1432 1004 BglI GCCn_nnn'nGGC Cuts at: 0 729 1465 2436 Size: 729 736 971 Fragments arranged by size: 971 736 729 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1214 1594 2436 Size: 1214 380 842 Fragments arranged by size: 1214 842 380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 1184 2436 Size: 385 799 1252 Fragments arranged by size: 1252 799 385 Bpu10I CC'TnA_GC Cuts at: 0 650 2436 Size: 650 1786 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1940 2436 Size: 1940 496 BsaI GGTCTCn'nnnn_ Cuts at: 0 41 2089 2436 Size: 41 2048 347 Fragments arranged by size: 2048 347 41 BsaAI yAC'GTr Cuts at: 0 1318 2436 Size: 1318 1118 BsaHI Gr'CG_yC Cuts at: 0 1106 2436 Size: 1106 1330 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1913 1943 2436 Size: 1913 30 493 Fragments arranged by size: 1913 493 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 449 592 2326 2436 Size: 344 105 143 1734 110 Fragments arranged by size: 1734 344 143 110 105 BsiHKAI G_wGCw'C Cuts at: 0 234 480 1970 2127 2384 2436 Size: 234 246 1490 157 257 52 Fragments arranged by size: 1490 257 246 234 157 52 BsmI GAATG_Cn' Cuts at: 0 464 1432 2424 2436 Size: 464 968 992 12 Fragments arranged by size: 992 968 464 12 Bsp1286I G_dGCh'C Cuts at: 0 234 480 1970 2127 2172 2384 2436 Size: 234 246 1490 157 45 212 52 Fragments arranged by size: 1490 246 234 212 157 52 45 BsrDI GCAATG_nn' Cuts at: 0 1244 1982 2436 Size: 1244 738 454 Fragments arranged by size: 1244 738 454 BsrGI T'GTAC_A Cuts at: 0 1920 2436 Size: 1920 516 BssSI C'ACGA_G Cuts at: 0 272 2436 Size: 272 2164 BstBI TT'CG_AA Cuts at: 0 285 423 2436 Size: 285 138 2013 Fragments arranged by size: 2013 285 138 BtgI C'CryG_G Cuts at: 0 1764 2436 Size: 1764 672 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1980 2436 Size: 1980 456 DraI TTT'AAA Cuts at: 0 29 710 2436 Size: 29 681 1726 Fragments arranged by size: 1726 681 29 DraIII CAC_nnn'GTG Cuts at: 0 615 2436 Size: 615 1821 EaeI y'GGCC_r Cuts at: 0 1562 2139 2436 Size: 1562 577 297 Fragments arranged by size: 1562 577 297 EarI CTCTTCn'nnn_ Cuts at: 0 134 613 1361 1975 2177 2436 Size: 134 479 748 614 202 259 Fragments arranged by size: 748 614 479 259 202 134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 481 2436 Size: 481 1955 EcoICRI GAG'CTC Cuts at: 0 232 2382 2436 Size: 232 2150 54 Fragments arranged by size: 2150 232 54 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 707 1184 1319 1360 2198 2436 Size: 385 322 477 135 41 838 238 Fragments arranged by size: 838 477 385 322 238 135 41 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 651 1390 2436 Size: 651 739 1046 Fragments arranged by size: 1046 739 651 FspI TGC'GCA Cuts at: 0 1622 2436 Size: 1622 814 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 304 336 366 398 2436 Size: 304 32 30 32 2038 Fragments arranged by size: 2038 304 32 32 30 HindIII A'AGCT_T Cuts at: 0 1301 2436 Size: 1301 1135 MfeI C'AATT_G Cuts at: 0 266 1544 2436 Size: 266 1278 892 Fragments arranged by size: 1278 892 266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 364 1619 2091 2180 2436 Size: 364 1255 472 89 256 Fragments arranged by size: 1255 472 364 256 89 MscI TGG'CCA Cuts at: 0 1564 2436 Size: 1564 872 MspA1I CmG'CkG Cuts at: 0 349 1820 2436 Size: 349 1471 616 Fragments arranged by size: 1471 616 349 NdeI CA'TA_TG Cuts at: 0 1551 2436 Size: 1551 885 NsiI A_TGCA'T Cuts at: 0 1434 2436 Size: 1434 1002 NspI r_CATG'y Cuts at: 0 484 1282 1527 1697 2254 2285 2436 Size: 484 798 245 170 557 31 151 Fragments arranged by size: 798 557 484 245 170 151 31 PciI A'CATG_T Cuts at: 0 1693 2436 Size: 1693 743 PfoI T'CCnGG_A Cuts at: 0 1096 2436 Size: 1096 1340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1510 1542 2436 Size: 1510 32 894 Fragments arranged by size: 1510 894 32 PsiI TTA'TAA Cuts at: 0 814 2436 Size: 814 1622 PstI C_TGCA'G Cuts at: 0 1856 1981 2436 Size: 1856 125 455 Fragments arranged by size: 1856 455 125 PvuII CAG'CTG Cuts at: 0 349 2436 Size: 349 2087 SacI G_AGCT'C Cuts at: 0 234 2384 2436 Size: 234 2150 52 Fragments arranged by size: 2150 234 52 SapI GCTCTTCn'nnn_ Cuts at: 0 613 1975 2177 2436 Size: 613 1362 202 259 Fragments arranged by size: 1362 613 259 202 ScaI AGT'ACT Cuts at: 0 1205 2436 Size: 1205 1231 SfcI C'TryA_G Cuts at: 0 1322 1852 1977 2436 Size: 1322 530 125 459 Fragments arranged by size: 1322 530 459 125 SmlI C'TyrA_G Cuts at: 0 1273 1955 2436 Size: 1273 682 481 Fragments arranged by size: 1273 682 481 SnaBI TAC'GTA Cuts at: 0 1318 2436 Size: 1318 1118 SphI G_CATG'C Cuts at: 0 1282 2254 2436 Size: 1282 972 182 Fragments arranged by size: 1282 972 182 SspI AAT'ATT Cuts at: 0 1055 1597 2436 Size: 1055 542 839 Fragments arranged by size: 1055 839 542 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 676 880 2436 Size: 676 204 1556 Fragments arranged by size: 1556 676 204 TatI w'GTAC_w Cuts at: 0 1203 1920 2436 Size: 1203 717 516 Fragments arranged by size: 1203 717 516 Enzymes that do cut: AcuI AflII AflIII AlfI AloI AlwNI ApoI BanI BanII BbsI BbvCI BfrBI BglI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrGI BssSI BstBI BtgI BtgZI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI FalI FspI Hin4I HindIII MfeI MmeI MscI MspA1I NdeI NsiI NspI PciI PfoI PpiI PsiI PstI PvuII SacI SapI ScaI SfcI SmlI SnaBI SphI SspI TaqII TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AhdI AleI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BsaBI BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII HincII HpaI KasI KpnI MluI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI