(Linear) (Six-base) MAPSORT of: KIEE2447.seq Check: 5826 from: 1 to: 2556 KIEE2447.orf With 182 enzymes: SgfI * January 7, 2013 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1375 2556 Size: 1375 1181 AccI GT'mk_AC Cuts at: 0 468 2377 2556 Size: 468 1909 179 Fragments arranged by size: 1909 468 179 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 902 1690 2207 2413 2556 Size: 77 825 788 517 206 143 Fragments arranged by size: 825 788 517 206 143 77 AhdI GACnn_n'nnGTC Cuts at: 0 2430 2556 Size: 2430 126 AleI CACnn'nnGTG Cuts at: 0 2413 2556 Size: 2413 143 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1901 1933 2556 Size: 1901 32 623 Fragments arranged by size: 1901 623 32 ApaI G_GGCC'C Cuts at: 0 615 2556 Size: 615 1941 ApaLI G'TGCA_C Cuts at: 0 798 2556 Size: 798 1758 ApoI r'AATT_y Cuts at: 0 63 330 396 733 1118 1604 2048 2306 Size: 63 267 66 337 385 486 444 258 Cuts at: 2306 2505 2556 Size: 199 51 Fragments arranged by size: 486 444 385 337 267 258 199 66 63 51 AvaI C'yCGr_G Cuts at: 0 1741 2556 Size: 1741 815 BamHI G'GATC_C Cuts at: 0 894 2556 Size: 894 1662 BanII G_rGCy'C Cuts at: 0 604 615 811 2556 Size: 604 11 196 1745 Fragments arranged by size: 1745 604 196 11 BclI T'GATC_A Cuts at: 0 2043 2556 Size: 2043 513 BglII A'GATC_T Cuts at: 0 406 2556 Size: 406 2150 Bme1580I G_kGCm'C Cuts at: 0 615 710 802 2556 Size: 615 95 92 1754 Fragments arranged by size: 1754 615 95 92 BmgBI CAC'GTC Cuts at: 0 2194 2556 Size: 2194 362 BmrI ACTGGGnnnn_n' Cuts at: 0 1139 2556 Size: 1139 1417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 883 1223 2268 2348 2403 2493 2507 2556 Size: 883 340 1045 80 55 90 14 49 Fragments arranged by size: 1045 883 340 90 80 55 49 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 375 1038 2556 Size: 375 663 1518 Fragments arranged by size: 1518 663 375 BsaBI GATnn'nnATC Cuts at: 0 365 2042 2556 Size: 365 1677 514 Fragments arranged by size: 1677 514 365 BsaWI w'CCGG_w Cuts at: 0 346 2556 Size: 346 2210 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 656 686 2556 Size: 656 30 1870 Fragments arranged by size: 1870 656 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1729 2556 Size: 1729 827 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1031 2556 Size: 1031 1525 BsiHKAI G_wGCw'C Cuts at: 0 802 1430 2391 2556 Size: 802 628 961 165 Fragments arranged by size: 961 802 628 165 BsmI GAATG_Cn' Cuts at: 0 121 2533 2556 Size: 121 2412 23 Fragments arranged by size: 2412 121 23 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2200 2556 Size: 2200 356 Bsp1286I G_dGCh'C Cuts at: 0 604 615 710 802 811 1430 2391 2556 Size: 604 11 95 92 9 619 961 165 Fragments arranged by size: 961 619 604 165 95 92 11 9 BspHI T'CATG_A Cuts at: 0 911 2556 Size: 911 1645 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1375 2367 2556 Size: 1375 992 189 Fragments arranged by size: 1375 992 189 BsrBI CCG'CTC Cuts at: 0 569 2556 Size: 569 1987 BsrGI T'GTAC_A Cuts at: 0 1824 2556 Size: 1824 732 BstEII G'GTnAC_C Cuts at: 0 633 1662 2556 Size: 633 1029 894 Fragments arranged by size: 1029 894 633 BstYI r'GATC_y Cuts at: 0 294 406 485 894 2556 Size: 294 112 79 409 1662 Fragments arranged by size: 1662 409 294 112 79 BstZ17I GTA'TAC Cuts at: 0 469 2378 2556 Size: 469 1909 178 Fragments arranged by size: 1909 469 178 BtsI GCAGTG_nn' Cuts at: 0 1682 1827 2556 Size: 1682 145 729 Fragments arranged by size: 1682 729 145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1095 1130 2556 Size: 1095 35 1426 Fragments arranged by size: 1426 1095 35 DraI TTT'AAA Cuts at: 0 960 1928 2556 Size: 960 968 628 Fragments arranged by size: 968 960 628 EaeI y'GGCC_r Cuts at: 0 145 2556 Size: 145 2411 EarI CTCTTCn'nnn_ Cuts at: 0 259 1067 2165 2556 Size: 259 808 1098 391 Fragments arranged by size: 1098 808 391 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 758 2556 Size: 758 1798 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 77 883 902 1223 1690 2207 2268 2348 Size: 77 806 19 321 467 517 61 80 Cuts at: 2348 2403 2413 2493 2507 2556 Size: 55 10 80 14 49 Fragments arranged by size: 806 517 467 321 80 80 77 61 55 49 19 14 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 2315 2556 Size: 2315 241 EcoO109I rG'GnC_Cy Cuts at: 0 123 952 2556 Size: 123 829 1604 Fragments arranged by size: 1604 829 123 EcoRI G'AATT_C Cuts at: 0 733 2556 Size: 733 1823 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2050 2082 2416 2448 2556 Size: 2050 32 334 32 108 Fragments arranged by size: 2050 334 108 32 32 HincII GTy'rAC Cuts at: 0 553 2556 Size: 553 2003 HindIII A'AGCT_T Cuts at: 0 2233 2556 Size: 2233 323 MfeI C'AATT_G Cuts at: 0 141 1112 2556 Size: 141 971 1444 Fragments arranged by size: 1444 971 141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 797 1358 1543 2556 Size: 797 561 185 1013 Fragments arranged by size: 1013 797 561 185 MslI CAynn'nnrTG Cuts at: 0 2413 2556 Size: 2413 143 MspA1I CmG'CkG Cuts at: 0 561 1075 2227 2556 Size: 561 514 1152 329 Fragments arranged by size: 1152 561 514 329 NdeI CA'TA_TG Cuts at: 0 2301 2556 Size: 2301 255 NspI r_CATG'y Cuts at: 0 457 780 2556 Size: 457 323 1776 Fragments arranged by size: 1776 457 323 PflMI CCAn_nnn'nTGG Cuts at: 0 1085 2556 Size: 1085 1471 PpuMI rG'GwC_Cy Cuts at: 0 123 2556 Size: 123 2433 PshAI GACnn'nnGTC Cuts at: 0 1019 2556 Size: 1019 1537 PsiI TTA'TAA Cuts at: 0 533 986 2089 2556 Size: 533 453 1103 467 Fragments arranged by size: 1103 533 467 453 PspOMI G'GGCC_C Cuts at: 0 611 2556 Size: 611 1945 PvuII CAG'CTG Cuts at: 0 561 1075 2227 2556 Size: 561 514 1152 329 Fragments arranged by size: 1152 561 514 329 SapI GCTCTTCn'nnn_ Cuts at: 0 1067 2165 2556 Size: 1067 1098 391 Fragments arranged by size: 1098 1067 391 SfcI C'TryA_G Cuts at: 0 309 2556 Size: 309 2247 SmlI C'TyrA_G Cuts at: 0 390 1017 2556 Size: 390 627 1539 Fragments arranged by size: 1539 627 390 SpeI A'CTAG_T Cuts at: 0 2499 2556 Size: 2499 57 SspI AAT'ATT Cuts at: 0 108 276 1714 2556 Size: 108 168 1438 842 Fragments arranged by size: 1438 842 168 108 StyI C'CwwG_G Cuts at: 0 711 1964 2556 Size: 711 1253 592 Fragments arranged by size: 1253 711 592 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 17 2556 Size: 17 2539 TatI w'GTAC_w Cuts at: 0 672 1824 2556 Size: 672 1152 732 Fragments arranged by size: 1152 732 672 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2183 2215 2556 Size: 2183 32 341 Fragments arranged by size: 2183 341 32 XmnI GAAnn'nnTTC Cuts at: 0 1394 2556 Size: 1394 1162 Enzymes that do cut: AarI AccI AcuI AhdI AleI AloI ApaI ApaLI ApoI AvaI BamHI BanII BclI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BstEII BstYI BstZ17I BtsI CspCI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII MfeI MmeI MslI MspA1I NdeI NspI PflMI PpuMI PshAI PsiI PspOMI PvuII SapI SfcI SmlI SpeI SspI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI AlwNI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI BmtI BplI Bpu10I BsaI BsaAI BsaHI BseYI BsiEI BsiWI BspEI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI Bsu36I BtgI BtgZI ClaI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MluI MscI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI