################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 102 ACATGT A^C[GA][CT]GT Alw44I 264 GTGCAC G^TGCAC AlwI 195 GGATC GGATC(4/5) ApaLI 264 GTGCAC G^TGCAC AvaI 206 CTCGGG C^[CT]CG[GA]G BalI 258 TGGCCA TGG^CCA BanII 378 GAGCTC G[GA]GC[CT]^C BglI 440 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 366 CTGGAG CTGGAG(16/14) BsaJI 383 CCCAGG C^C[ACGT][ACGT]GG BsoBI 206 CTCGGG C^[CT]CG[GA]G BspLU11I 102 ACATGT A^CATGT BspMI 549 ACCTGC ACCTGC(4/8) BsrBI 577 GAGCGG CCGCTC(-3/-3) BstF5I 45 CATCC GGATG(2/0) BstNI 384 CCAGG CC^[AT]GG DpnII 196 GATC ^GATC DraI 70 TTTAAA TTT^AAA EaeI 258 TGGCCA [CT]^GGCC[GA] EcoRII 384 CCAGG ^CC[AT]GG EcoT22I 42 ATGCAT ATGCA^T FokI 45 CATCC GGATG(9/13) HaeIII 259 GGCC GG^CC HphI 547 TCACC GGTGA(8/7) MaeII 145 ACGT A^CGT MboI 196 GATC ^GATC MluNI 258 TGGCCA TGG^CCA MscI 258 TGGCCA TGG^CCA MslI 45 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 430 CAGCTG C[AC]G^C[GT]G MvaI 384 CCAGG CC^[AT]GG NdeII 196 GATC ^GATC NsiI 42 ATGCAT ATGCA^T PleI 520 GAGTC GAGTC(4/5) PstI 433 CTGCAG CTGCA^G PvuII 430 CAGCTG CAG^CTG SacI 378 GAGCTC GAGCT^C Sau3AI 196 GATC ^GATC ScaI 362 AGTACT AGT^ACT SfcI 433 CTGCAG C^T[GA][CT]AG SphI 40 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 393 GCGG CCGC(-3/-1) 579 GCGG BcnI 225 CCGGG CC^[GC]GG 506 CCCGG BseRI 344 GAGGAG GAGGAG(10/8) 375 GAGGAG BsiYI 279 CCCTCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 383 CCCAGGAGTGG BslI 279 CCCTCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 383 CCCAGGAGTGG HapII 225 CCGG C^CGG 507 CCGG HpaII 225 CCGG C^CGG 507 CCGG MseI 71 TTAA T^TAA 110 TTAA MspI 225 CCGG C^CGG 507 CCGG NciI 225 CCGGG CC^[GC]GG 506 CCCGG TfiI 150 GAATC G^A[AT]TC 425 GAATC Tru9I 71 TTAA T^TAA 110 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AfaI AluI AspHI BbvI BmyI BsiHKAI Bsp1286I BsrI BssKI Cac8I Fnu4HI HinfI ItaI MaeIII MnlI MwoI NlaIII NspI RsaI ScrFI SfaNI TseI Tsp45I TspRI