################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbvCI BcgI BciVI BfaI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BsrI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 39 CGCG CG^CG AflII 955 CTTAAG C^TTAAG AscI 37 GGCGCGCC GG^CGCGCC Asp700I 487 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 441 GGTCC G^G[AT]CC BbrPI 127 CACGTG CAC^GTG BbsI 579 GAAGAC GAAGAC(2/6) BclI 368 TGATCA T^GATCA BfrI 955 CTTAAG C^TTAAG BpmI 853 CTCCAG CTGGAG(16/14) BpuAI 579 GAAGAC GAAGAC(2/6) BsaAI 127 CACGTG [CT]AC^GT[GA] BseRI 465 CTCCTC GAGGAG(10/8) BsmAI 150 GTCTC GTCTC(1/5) BsmFI 168 GTCCC GGGAC(10/14) BspMI 64 ACCTGC ACCTGC(4/8) BsrBI 768 CCGCTC CCGCTC(-3/-3) BsrDI 636 CATTGC GCAATG(2/0) BsrFI 74 ACCGGC [GA]^CCGG[CT] BssHII 38 GCGCGC G^CGCGC BstAPI 414 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 39 CGCG CG^CG BstXI 308 CCAATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 74 ACCGGC [GA]^CCGG[CT] DraI 186 TTTAAA TTT^AAA DraII 440 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 449 CCACGG C^C[GA][CT]GG EcoO109I 440 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 324 GAATTC G^AATTC EcoT14I 799 CCTTGG C^C[AT][AT]GG FbaI 368 TGATCA T^GATCA HgaI 582 GACGC GACGC(5/10) HincII 682 GTCAAC GT[CT]^[GA]AC HindII 682 GTCAAC GT[CT]^[GA]AC HphI 821 TCACC GGTGA(8/7) MslI 63 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 39 CGCG CG^CG NspI 68 GCATGT [GA]CATG^[CT] PmaCI 127 CACGTG CAC^GTG PmlI 127 CACGTG CAC^GTG PpuMI 440 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 544 CAGCTG CAG^CTG SapI 792 GAAGAGC GCTCTTC(1/4) SexAI 11 ACCAGGT A^CC[AT]GGT StyI 799 CCTTGG C^C[AT][AT]GG TaqI 620 TCGA T^CGA Tsp45I 877 GTGAC ^GT[GC]AC TthHB8I 620 TCGA T^CGA XcmI 305 CCACCAATGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 487 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 324 GAATTC [GA]^AATT[CT] 592 AAATTC Alw44I 88 GTGCAC G^TGCAC 454 GTGCAC ApaLI 88 GTGCAC G^TGCAC 454 GTGCAC ApoI 324 GAATTC [GA]^AATT[CT] 592 AAATTC BalI 123 TGGCCA TGG^CCA 663 TGGCCA BanII 289 GAGCCC G[GA]GC[CT]^C 795 GAGCCC BcnI 115 CCGGG CC^[GC]GG 381 CCCGG BstNI 12 CCAGG CC^[AT]GG 924 CCTGG DpnII 369 GATC ^GATC 861 GATC EaeI 123 TGGCCA [CT]^GGCC[GA] 663 TGGCCA EarI 136 GAAGAG CTCTTC(1/4) 792 GAAGAG EcoRII 12 CCAGG ^CC[AT]GG 924 CCTGG FauI 170 CCCGC CCCGC(4/6) 530 CCCGC Ksp632I 136 GAAGAG CTCTTC(1/4) 792 GAAGAG MaeIII 265 GTAAC ^GT[ACGT]AC 877 GTGAC MboI 369 GATC ^GATC 861 GATC MluNI 123 TGGCCA TGG^CCA 663 TGGCCA MscI 123 TGGCCA TGG^CCA 663 TGGCCA MspA1I 544 CAGCTG C[AC]G^C[GT]G 758 CCGCTG MvaI 12 CCAGG CC^[AT]GG 924 CCTGG NciI 115 CCGGG CC^[GC]GG 381 CCCGG NdeII 369 GATC ^GATC 861 GATC NlaIII 69 CATG CATG^ 774 CATG PleI 139 GAGTC GAGTC(4/5) 166 GAGTC PstI 212 CTGCAG CTGCA^G 870 CTGCAG Sau3AI 369 GATC ^GATC 861 GATC SfaNI 250 GATGC GCATC(5/9) 599 GCATC SfcI 212 CTGCAG C^T[GA][CT]AG 870 CTGCAG TfiI 237 GATTC G^A[AT]TC 551 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AfaI AluI AspHI BbvI BmyI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I Cac8I CfoI Cfr13I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboII MnlI MseI MspI MwoI NlaIV RsaI Sau96I ScrFI SmlI Tru9I TseI Tsp509I TspRI