################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflIII AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfaI BglII BlnI BsaAI BsaHI BseAI BsiEI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI BstBI BstYI ClaI CpoI DpnII DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI Ksp632I KspI MaeI MaeII MboI MfeI MflI MluI MroI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 335 GGTACC G^GTACC AccII 96 CGCG CG^CG AflII 361 CTTAAG C^TTAAG AgeI 332 ACCGGT A^CCGGT AocI 382 CCTCAGG CC^T[ACGT]AGG Asp718I 335 GGTACC G^GTACC BbsI 886 GTCTTC GAAGAC(2/6) BbvCI 285 CCTCAGC CCTCAGC(-5/-2) BfrI 361 CTTAAG C^TTAAG BlpI 17 GCTCAGC GC^T[ACGT]AGC Bpu1102I 17 GCTCAGC GC^T[ACGT]AGC BpuAI 886 GTCTTC GAAGAC(2/6) BsaBI 732 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 399 GGTCTC GGTCTC(1/5) BsaWI 332 ACCGGT [AT]^CCGG[AT] BseRI 380 CTCCTC GAGGAG(10/8) BsiWI 143 CGTACG C^GTACG BsrDI 587 GCAATG GCAATG(2/0) BsrFI 332 ACCGGT [GA]^CCGG[CT] BsrI 666 ACTGG ACTGG(1/-1) Bst1107I 841 GTATAC GTA^TAC BstAPI 819 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 669 GGTCACC G^GT[ACGT]ACC BstPI 669 GGTCACC G^GT[ACGT]ACC BstUI 96 CGCG CG^CG BstZ17I 841 GTATAC GTA^TAC Bsu36I 382 CCTCAGG CC^T[ACGT]AGG CelII 17 GCTCAGC GC^T[ACGT]AGC Cfr10I 332 ACCGGT [GA]^CCGG[CT] Eco81I 382 CCTCAGG CC^T[ACGT]AGG EcoNI 875 CCTATCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 669 GGTCACC G^GT[ACGT]ACC EcoT14I 674 CCTTGG C^C[AT][AT]GG FauI 654 GCGGG CCCGC(4/6) HaeII 102 GGCGCT [GA]GCGC^[CT] HincII 367 GTCAAC GT[CT]^[GA]AC HindII 367 GTCAAC GT[CT]^[GA]AC HindIII 646 AAGCTT A^AGCTT KpnI 335 GGTACC GGTAC^C MamI 732 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 362 TTAA T^TAA MvnI 96 CGCG CG^CG PinAI 332 ACCGGT A^CCGGT PstI 759 CTGCAG CTGCA^G PvuII 44 CAGCTG CAG^CTG SacI 870 GAGCTC GAGCT^C SexAI 478 ACCAGGT A^CC[AT]GGT SfcI 759 CTGCAG C^T[GA][CT]AG SfiI 306 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 441 CCCGGG CCC^GGG SmlI 361 CTTAAG C^T[CT][GA]AG StyI 674 CCTTGG C^C[AT][AT]GG TfiI 747 GAATC G^A[AT]TC Tru9I 362 TTAA T^TAA XcmI 26 CCAGCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 441 CCCGGG C^CCGGG XmaI 441 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 151 GTCTAC GT^[AC][GT]AC 841 GTATAC AfaI 144 GTAC GT^AC 336 GTAC AlwNI 661 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG 745 CAGAATCTG AspHI 826 GTGCTC G[AT]GC[AT]^C 870 GAGCTC AvaI 402 CTCGGG C^[CT]CG[GA]G 441 CCCGGG BalI 38 TGGCCA TGG^CCA 719 TGGCCA BanI 335 GGTACC G^G[CT][GA]CC 482 GGTGCC BsgI 817 CTGCAC GTGCAG(16/14) 773 GTGCAG BsiHKAI 826 GTGCTC G[AT]GC[AT]^C 870 GAGCTC BsoBI 402 CTCGGG C^[CT]CG[GA]G 441 CCCGGG BstF5I 302 GGATG GGATG(2/0) 792 GGATG BstXI 26 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 41 CCACAGCTGTGG CfoI 103 GCGC GCG^C 270 GCGC DraIII 477 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 820 CACAGTGTG DsaI 320 CCGTGG C^C[GA][CT]GG 836 CCGTGG FokI 302 GGATG GGATG(9/13) 792 GGATG HhaI 103 GCGC GCG^C 270 GCGC HinP1I 103 GCGC G^CGC 270 GCGC MaeIII 670 GTCAC ^GT[ACGT]AC 796 GTCAC MboII 740 TCTTC GAAGA(8/7) 887 TCTTC MluNI 38 TGGCCA TGG^CCA 719 TGGCCA MscI 38 TGGCCA TGG^CCA 719 TGGCCA MspA1I 44 CAGCTG C[AC]G^C[GT]G 652 CAGCGG PflMI 660 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 744 CCAGAATCTGG RsaI 144 GTAC GT^AC 336 GTAC Tsp45I 670 GTCAC ^GT[GC]AC 796 GTCAC Van91I 660 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 744 CCAGAATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI ApaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MnlI MslI MspI MvaI MwoI NciI NlaIV PleI Sau96I ScrFI SfaNI TseI TspRI