(Circular) (Six-base) MAPSORT of: pFN21AE9410.seq Check: 4715 from: 1 to: 5662 pFN21AE9410 5662 bp With 182 enzymes: SgfI * July 27, 2011 11:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2450 2450 Size: 5662 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2157 2847 3005 1937 Size: 220 690 158 4594 Fragments arranged by size: 4594 690 220 158 Acc65I G'GTAC_C Cuts at: 2340 2983 2340 Size: 643 5019 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2506 2540 2639 2854 3725 5109 5377 1632 Size: 874 34 99 215 871 1384 268 1917 Fragments arranged by size: 1917 1384 874 871 268 215 99 34 AflII C'TTAA_G Cuts at: 820 1017 2366 820 Size: 197 1349 4116 Fragments arranged by size: 4116 1349 197 AflIII A'CryG_T Cuts at: 1203 4561 5538 1203 Size: 3358 977 1327 Fragments arranged by size: 3358 1327 977 AgeI A'CCGG_T Cuts at: 2337 4493 2337 Size: 2156 3506 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2039 2051 2681 1166 Size: 873 12 630 4147 Fragments arranged by size: 4147 873 630 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2566 1184 Size: 1382 4280 AlwNI CAG_nnn'CTG Cuts at: 2671 2755 3405 4977 2671 Size: 84 650 1572 3356 Fragments arranged by size: 3356 1572 650 84 ApaI G_GGCC'C Cuts at: 2272 2665 2773 2272 Size: 393 108 5161 Fragments arranged by size: 5161 393 108 ApaLI G'TGCA_C Cuts at: 3719 4875 3719 Size: 1156 4506 ApoI r'AATT_y Cuts at: 1493 2971 3197 3349 5458 1493 Size: 1478 226 152 2109 1697 Fragments arranged by size: 2109 1697 1478 226 152 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2407 2446 2987 1870 Size: 73 464 39 541 4545 Fragments arranged by size: 4545 541 464 73 39 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2332 2365 2332 Size: 33 5629 BamHI G'GATC_C Cuts at: 2992 3359 2992 Size: 367 5295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2340 2487 2983 4438 618 Size: 325 217 627 553 147 496 1455 1842 Fragments arranged by size: 1842 1455 627 553 496 325 217 147 BanII G_rGCy'C Cuts at: 1391 2272 2614 2665 2773 2879 2931 2981 1391 Size: 881 342 51 108 106 52 50 4072 Fragments arranged by size: 4072 881 342 108 106 52 51 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2884 928 Size: 971 985 3706 Fragments arranged by size: 3706 985 971 BbvCI CC'TCA_GC Cuts at: 2291 2291 Size: 5662 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3853 3887 1903 Size: 34 50 34 1832 34 3678 Fragments arranged by size: 3678 1832 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3554 4764 3554 Size: 1210 4452 BclI T'GATC_A Cuts at: 1548 1548 Size: 5662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2259 2318 2327 Size: 107 122 71 631 605 587 59 9 Cuts at: 2327 4273 136 Size: 1946 1525 Fragments arranged by size: 1946 1525 631 605 587 122 107 71 59 9 BglII A'GATC_T Cuts at: 5657 5657 Size: 5662 BlpI GC'TnA_GC Cuts at: 2023 3079 2023 Size: 1056 4606 Bme1580I G_kGCm'C Cuts at: 1790 2272 2665 2773 3723 4879 1790 Size: 482 393 108 950 1156 2573 Fragments arranged by size: 2573 1156 950 482 393 108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2219 2488 2600 2842 4322 1239 Size: 854 126 269 112 242 1480 2579 Fragments arranged by size: 2579 1480 854 269 242 126 112 Bpu10I CC'TnA_GC Cuts at: 2228 2291 2228 Size: 63 5599 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3787 4652 4950 3787 Size: 865 298 4499 Fragments arranged by size: 4499 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2410 4325 882 Size: 617 911 1915 2219 Fragments arranged by size: 2219 1915 911 617 BsaAI yAC'GTr Cuts at: 493 1204 3453 493 Size: 711 2249 2702 Fragments arranged by size: 2702 2249 711 BsaBI GATnn'nnATC Cuts at: 2741 5656 2741 Size: 2915 2747 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3469 3851 4486 275 Size: 53 83 186 936 1936 382 635 1451 Fragments arranged by size: 1936 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2337 4095 4493 4767 4914 5325 2337 Size: 1758 398 274 147 411 2674 Fragments arranged by size: 2674 1758 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2840 2870 1178 Size: 30 165 30 1437 30 3970 Fragments arranged by size: 3970 1437 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2376 1186 Size: 1190 4472 BseYI C'CCAG_C Cuts at: 2030 2065 2083 2092 2265 4865 5361 2030 Size: 35 18 9 173 2600 496 2331 Fragments arranged by size: 2600 2331 496 173 35 18 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2799 2807 1163 Size: 573 1063 8 4018 Fragments arranged by size: 4018 1063 573 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2835 2879 3723 3808 4879 2835 Size: 44 844 85 1071 3618 Fragments arranged by size: 3618 1071 844 85 44 BsiWI C'GTAC_G Cuts at: 2148 2148 Size: 5662 BsmI GAATG_Cn' Cuts at: 3173 3266 3173 Size: 93 5569 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2272 2614 2665 2773 2835 2879 2931 Size: 399 482 342 51 108 62 44 52 Cuts at: 2931 2981 3723 3808 4879 1391 Size: 50 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 342 108 85 62 52 51 50 44 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 2193 2385 2450 2829 3017 844 Size: 1325 24 192 65 379 188 3489 Fragments arranged by size: 3489 1325 379 192 188 65 24 BsrBI CCG'CTC Cuts at: 1394 1823 3549 1394 Size: 429 1726 3507 Fragments arranged by size: 3507 1726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2599 4157 4331 65 Size: 1183 1351 1558 174 1396 Fragments arranged by size: 1558 1396 1351 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2337 4306 4493 1700 Size: 110 527 1969 187 2869 Fragments arranged by size: 2869 1969 527 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 2830 794 Size: 2036 3626 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 2674 1184 Size: 1490 4172 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2053 2038 Size: 15 5647 BstYI r'GATC_y Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657 Size: 1020 367 385 17 1452 86 12 346 Cuts at: 5657 1972 Size: 1977 Fragments arranged by size: 1977 1452 1020 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2848 2848 Size: 5662 Bsu36I CC'TnA_GG Cuts at: 2388 2388 Size: 5662 BtgI C'CryG_G Cuts at: 513 1065 2325 2841 513 Size: 552 1260 516 3334 Fragments arranged by size: 3334 1260 552 516 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 2787 3178 3972 3992 2787 Size: 391 794 20 4457 Fragments arranged by size: 4457 794 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DraIII CAC_nnn'GTG Cuts at: 2487 2830 2487 Size: 343 5319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2214 2319 Size: 54 1425 50 275 18 213 171 105 Cuts at: 2319 2724 3109 3998 5627 8 Size: 405 385 889 1629 43 Fragments arranged by size: 1629 1425 889 405 385 275 213 171 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3592 1353 Size: 2239 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2877 2877 Size: 5662 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2219 2488 2506 2540 2600 2639 Size: 393 461 126 269 18 34 60 39 Cuts at: 2639 2842 2854 3725 4322 5109 5377 1239 Size: 203 12 871 597 787 268 1524 Fragments arranged by size: 1524 871 787 597 461 393 269 268 203 126 60 39 34 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2884 2884 Size: 5662 EcoO109I rG'GnC_Cy Cuts at: 2269 2769 2770 2269 Size: 500 1 5161 Fragments arranged by size: 5161 500 1 EcoRI G'AATT_C Cuts at: 2971 3349 2971 Size: 378 5284 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 2111 4809 1678 Size: 433 2698 2531 Fragments arranged by size: 2698 2531 433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2866 2898 4303 4335 4377 Size: 32 135 32 1294 32 1405 32 42 Cuts at: 4377 4409 1373 Size: 32 2626 Fragments arranged by size: 2626 1405 1294 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2374 3006 3252 669 Size: 988 281 436 632 246 3079 Fragments arranged by size: 3079 988 632 436 281 246 HindIII A'AGCT_T Cuts at: 748 2651 748 Size: 1903 3759 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2344 2987 2344 Size: 643 5019 MfeI C'AATT_G Cuts at: 3261 3261 Size: 5662 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4776 4960 1305 Size: 3471 184 2007 Fragments arranged by size: 3471 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 2726 10 Size: 54 1425 343 213 681 2946 Fragments arranged by size: 2946 1425 681 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2051 2681 3620 3979 4138 518 Size: 648 873 12 630 939 359 159 2042 Fragments arranged by size: 2042 939 873 648 630 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2051 2659 3078 3755 4903 5148 1606 Size: 445 608 419 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 608 445 419 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5110 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 3020 4565 1132 Size: 97 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 97 PasI CC'CwG_GG Cuts at: 1348 2398 1348 Size: 1050 4612 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 2755 1108 Size: 1563 84 4015 Fragments arranged by size: 4015 1563 84 PfoI T'CCnGG_A Cuts at: 2628 2628 Size: 5662 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PspOMI G'GGCC_C Cuts at: 2268 2661 2769 2268 Size: 393 108 5161 Fragments arranged by size: 5161 393 108 PstI C_TGCA'G Cuts at: 830 2768 3014 830 Size: 1938 246 3478 Fragments arranged by size: 3478 1938 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2051 2051 Size: 5662 SacI G_AGCT'C Cuts at: 2879 2879 Size: 5662 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SbfI CC_TGCA'GG Cuts at: 3014 3014 Size: 5662 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SexAI A'CCwGG_T Cuts at: 2483 2483 Size: 5662 SfcI C'TryA_G Cuts at: 826 1045 2764 3010 4145 4826 5017 826 Size: 219 1719 246 1135 681 191 1471 Fragments arranged by size: 1719 1471 1135 681 246 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2318 2318 Size: 5662 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2448 2989 1872 Size: 576 541 4545 Fragments arranged by size: 4545 576 541 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 3766 4667 4929 820 Size: 197 926 423 1400 901 262 1553 Fragments arranged by size: 1553 1400 926 901 423 262 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SrfI GCCC'GGGC Cuts at: 2448 2448 Size: 5662 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 2679 3101 513 Size: 552 1614 422 3074 Fragments arranged by size: 3074 1614 552 422 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 3689 3706 3859 4044 4489 325 Size: 1255 465 1644 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1255 465 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3908 5620 96 Size: 274 80 33 51 1443 1931 1712 138 Fragments arranged by size: 1931 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5622 5654 5622 Size: 32 5630 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5662 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2038 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2446 2987 1870 Size: 576 541 4545 Fragments arranged by size: 4545 576 541 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsmBI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfoI SwaI