(Circular) (Six-base) MAPSORT of: pFN21AE2716.seq Check: 6445 from: 1 to: 5410 pFN21AE2716 5410 bp With 182 enzymes: SgfI * January 30, 2018 12:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2680 2680 Size: 5410 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3220 278 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2731 2731 Size: 5410 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3473 4857 5125 1632 Size: 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 4309 5286 1203 Size: 3106 977 1327 Fragments arranged by size: 3106 1327 977 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 2626 4139 2626 Size: 1513 3897 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5410 AlwNI CAG_nnn'CTG Cuts at: 2394 3153 4725 2394 Size: 759 1572 3079 Fragments arranged by size: 3079 1572 759 ApaLI G'TGCA_C Cuts at: 3467 4623 3467 Size: 1156 4254 ApoI r'AATT_y Cuts at: 1493 2464 2719 2945 3097 5206 1493 Size: 971 255 226 152 2109 1697 Fragments arranged by size: 2109 1697 971 255 226 152 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2735 1870 Size: 73 792 4545 Fragments arranged by size: 4545 792 73 BamHI G'GATC_C Cuts at: 2740 3107 2740 Size: 367 5043 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2731 4186 618 Size: 325 217 627 944 1455 1842 Fragments arranged by size: 1842 1455 944 627 325 217 BanII G_rGCy'C Cuts at: 1391 2729 1391 Size: 1338 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2337 2633 928 Size: 971 438 296 3705 Fragments arranged by size: 3705 971 438 296 BbvCI CC'TCA_GC Cuts at: 2414 2414 Size: 5410 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3601 3635 1903 Size: 34 50 34 1580 34 3678 Fragments arranged by size: 3678 1580 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2236 3302 4512 2236 Size: 1066 1210 3134 Fragments arranged by size: 3134 1210 1066 BclI T'GATC_A Cuts at: 1548 1548 Size: 5410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2391 4021 136 Size: 107 122 71 631 605 719 1630 1525 Fragments arranged by size: 1630 1525 719 631 605 122 107 71 BglII A'GATC_T Cuts at: 2336 5405 2336 Size: 3069 2341 BlpI GC'TnA_GC Cuts at: 2827 2827 Size: 5410 Bme1580I G_kGCm'C Cuts at: 1790 3471 4627 1790 Size: 1681 1156 2573 Fragments arranged by size: 2573 1681 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3141 4099 442 Size: 416 53 83 399 24 1724 958 1753 Fragments arranged by size: 1753 1724 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4070 1239 Size: 2831 2579 Bpu10I CC'TnA_GC Cuts at: 2414 2414 Size: 5410 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4400 4698 3535 Size: 865 298 4247 Fragments arranged by size: 4247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4073 882 Size: 617 2574 2219 Fragments arranged by size: 2574 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3201 493 Size: 711 1997 2702 Fragments arranged by size: 2702 1997 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3217 3599 4234 275 Size: 53 83 186 936 1684 382 635 1451 Fragments arranged by size: 1684 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5185 Fragments arranged by size: 5185 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5410 BseYI C'CCAG_C Cuts at: 4613 5109 4613 Size: 496 4914 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4837 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2860 3621 4649 1540 Size: 275 186 859 761 1028 2301 Fragments arranged by size: 2301 1028 859 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2308 3471 3556 4627 2308 Size: 1163 85 1071 3091 Fragments arranged by size: 3091 1163 1071 85 BsmI GAATG_Cn' Cuts at: 2696 2921 3014 2696 Size: 225 93 5092 Fragments arranged by size: 5092 225 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2308 2729 3471 3556 4627 1391 Size: 399 518 421 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 518 421 399 85 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 3299 5029 3299 Size: 1730 3680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2680 2765 844 Size: 1836 85 3489 Fragments arranged by size: 3489 1836 85 BsrBI CCG'CTC Cuts at: 1394 1823 3297 1394 Size: 429 1474 3507 Fragments arranged by size: 3507 1474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2525 3905 4079 65 Size: 1183 1277 1380 174 1396 Fragments arranged by size: 1396 1380 1277 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4054 4241 1700 Size: 110 2244 187 2869 Fragments arranged by size: 2869 2244 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5410 BssSI C'ACGA_G Cuts at: 3470 4482 3470 Size: 1012 4398 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5410 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstYI r'GATC_y Cuts at: 1972 2336 2740 3107 3492 3509 4961 5047 5059 Size: 364 404 367 385 17 1452 86 12 Cuts at: 5059 5405 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 404 385 367 364 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2180 2229 2356 513 Size: 552 1115 49 127 3567 Fragments arranged by size: 3567 1115 552 127 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4671 Fragments arranged by size: 4671 683 56 BtsI GCAGTG_nn' Cuts at: 2926 3720 3740 2926 Size: 794 20 4596 Fragments arranged by size: 4596 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4417 809 Size: 507 3101 1802 Fragments arranged by size: 3101 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2183 2421 2857 Size: 54 1425 50 275 18 353 238 436 Cuts at: 2857 3746 5375 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 436 353 275 238 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2857 1537 Size: 275 1045 4090 Fragments arranged by size: 4090 1045 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2290 3340 1353 Size: 937 1050 3423 Fragments arranged by size: 3423 1050 937 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3997 4371 4517 387 Size: 1064 138 2408 374 146 1280 Fragments arranged by size: 2408 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3473 4070 4857 5125 1239 Size: 393 1841 597 787 268 1524 Fragments arranged by size: 1841 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2203 2203 Size: 5410 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 FspI TGC'GCA Cuts at: 3916 3916 Size: 5410 HaeII r_GCGC'y Cuts at: 1678 4557 1678 Size: 2879 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2058 2090 2612 2644 4051 Size: 32 135 32 486 32 522 32 1407 Cuts at: 4051 4083 4125 4157 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1407 522 486 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2754 3000 669 Size: 988 281 816 246 3079 Fragments arranged by size: 3079 988 816 281 246 HindIII A'AGCT_T Cuts at: 748 2445 748 Size: 1697 3713 HpaI GTT'AAC Cuts at: 3000 3000 Size: 5410 KpnI G_GTAC'C Cuts at: 2735 2735 Size: 5410 MfeI C'AATT_G Cuts at: 3009 3009 Size: 5410 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4524 4708 1305 Size: 3219 184 2007 Fragments arranged by size: 3219 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2185 2423 10 Size: 54 1425 343 353 238 2997 Fragments arranged by size: 2997 1425 353 343 238 54 MslI CAynn'nnrTG Cuts at: 518 1166 3368 3727 3886 518 Size: 648 2202 359 159 2042 Fragments arranged by size: 2202 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2554 2826 3503 4651 4896 1606 Size: 948 272 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 677 272 245 NcoI C'CATG_G Cuts at: 513 1065 2180 2229 513 Size: 552 1115 49 3694 Fragments arranged by size: 3694 1115 552 49 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2857 2857 Size: 5410 NspI r_CATG'y Cuts at: 1132 1229 2768 4313 1132 Size: 97 1539 1545 2229 Fragments arranged by size: 2229 1545 1539 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5410 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5410 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2203 2203 Size: 5410 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PstI C_TGCA'G Cuts at: 830 2762 830 Size: 1932 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 PvuII CAG'CTG Cuts at: 2554 2554 Size: 5410 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SapI GCTCTTCn'nnn_ Cuts at: 2290 2290 Size: 5410 SbfI CC_TGCA'GG Cuts at: 2762 2762 Size: 5410 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SfcI C'TryA_G Cuts at: 826 1045 2758 3893 4574 4765 826 Size: 219 1713 1135 681 191 1471 Fragments arranged by size: 1713 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2737 1872 Size: 865 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3514 4415 4677 820 Size: 197 926 1571 901 262 1553 Fragments arranged by size: 1571 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SspI AAT'ATT Cuts at: 5 52 3334 5 Size: 47 3282 2081 Fragments arranged by size: 3282 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5410 StyI C'CwwG_G Cuts at: 513 1065 2180 2229 2849 513 Size: 552 1115 49 620 3074 Fragments arranged by size: 3074 1115 620 552 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 3437 3454 3607 3792 4237 325 Size: 1255 789 1068 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1068 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2056 3656 5368 Size: 274 80 33 51 1443 79 1600 1712 Cuts at: 5368 96 Size: 138 Fragments arranged by size: 1712 1600 1443 274 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5370 5402 5370 Size: 32 5378 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2193 2193 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5410 XmaI C'CCGG_G Cuts at: 1870 2735 1870 Size: 865 4545 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3218 276 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI