(Circular) (Six-base) MAPSORT of: pFN21AE6615.seq Check: 2298 from: 1 to: 5572 pFN21AE6615 5572 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 1937 Size: 978 4594 Acc65I G'GTAC_C Cuts at: 2802 2893 2802 Size: 91 5481 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3635 5019 5287 1632 Size: 2003 1384 268 1917 Fragments arranged by size: 2003 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 2309 4301 2309 Size: 1992 3580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2180 1184 Size: 996 4576 AlwNI CAG_nnn'CTG Cuts at: 3315 4887 3315 Size: 1572 4000 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2279 2881 3107 3259 5368 1493 Size: 786 602 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 602 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2383 2897 1870 Size: 73 440 514 4545 Fragments arranged by size: 4545 514 440 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2802 2824 2893 4348 618 Size: 325 217 627 1015 22 69 1455 1842 Fragments arranged by size: 1842 1455 1015 627 325 217 69 22 BanII G_rGCy'C Cuts at: 1391 2395 2406 2891 1391 Size: 1004 11 485 4072 Fragments arranged by size: 4072 1004 485 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2148 928 Size: 971 249 4352 Fragments arranged by size: 4352 971 249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2432 2466 3763 3797 1903 Size: 34 495 34 1297 34 3678 Fragments arranged by size: 3678 1297 495 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2554 3464 4674 2554 Size: 910 1210 3452 Fragments arranged by size: 3452 1210 910 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4183 136 Size: 107 122 71 631 605 2511 1525 Fragments arranged by size: 2511 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2989 2989 Size: 5572 Bme1580I G_kGCm'C Cuts at: 1790 3633 4789 1790 Size: 1843 1156 2573 Fragments arranged by size: 2573 1843 1156 BmgBI CAC'GTC Cuts at: 1318 2437 1318 Size: 1119 4453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2681 1056 Size: 1625 3947 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2409 2409 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4232 1239 Size: 2993 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2507 2819 3697 4562 4860 2507 Size: 312 878 865 298 3219 Fragments arranged by size: 3219 878 865 312 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2277 4235 882 Size: 617 778 1958 2219 Fragments arranged by size: 2219 1958 778 617 BsaAI yAC'GTr Cuts at: 493 1204 3363 493 Size: 711 2159 2702 Fragments arranged by size: 2702 2159 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3379 3761 4396 275 Size: 53 83 186 936 1846 382 635 1451 Fragments arranged by size: 1846 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2704 2734 1178 Size: 30 165 30 1301 30 4016 Fragments arranged by size: 4016 1301 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 2489 2712 1186 Size: 1294 9 223 4046 Fragments arranged by size: 4046 1294 223 9 BseYI C'CCAG_C Cuts at: 2508 2807 4775 5271 2508 Size: 299 1968 496 2809 Fragments arranged by size: 2809 1968 496 299 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2150 1163 Size: 573 414 4585 Fragments arranged by size: 4585 573 414 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3022 3783 4811 1540 Size: 275 186 1021 761 1028 2301 Fragments arranged by size: 2301 1028 1021 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2754 3633 3718 4789 2754 Size: 879 85 1071 3537 Fragments arranged by size: 3537 1071 879 85 BsmI GAATG_Cn' Cuts at: 2698 3083 3176 2698 Size: 385 93 5094 Fragments arranged by size: 5094 385 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2296 2296 Size: 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2406 2754 2891 3633 3718 4789 Size: 399 605 11 348 137 742 85 1071 Cuts at: 4789 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 605 399 348 137 85 11 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 2687 2927 844 Size: 1328 515 240 3489 Fragments arranged by size: 3489 1328 515 240 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4067 4241 65 Size: 1183 2819 174 1396 Fragments arranged by size: 2819 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4216 4403 1700 Size: 110 2406 187 2869 Fragments arranged by size: 2869 2406 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 2452 4376 2452 Size: 1924 3648 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2058 2902 3269 3654 3671 5123 5209 5221 Size: 86 844 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 844 385 367 346 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2796 2820 513 Size: 552 1731 24 3265 Fragments arranged by size: 3265 1731 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2489 2644 487 Size: 683 56 1263 155 3415 Fragments arranged by size: 3415 1263 683 155 56 BtsI GCAGTG_nn' Cuts at: 2164 3088 3882 3902 2164 Size: 924 794 20 3834 Fragments arranged by size: 3834 924 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2065 2876 2065 Size: 811 4761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3019 3908 5537 Size: 54 1425 50 275 18 1189 889 1629 Cuts at: 5537 8 Size: 43 Fragments arranged by size: 1629 1425 1189 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3019 1537 Size: 275 1207 4090 Fragments arranged by size: 4090 1207 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2466 3502 1353 Size: 1113 1036 3423 Fragments arranged by size: 3423 1113 1036 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3635 4232 5019 5287 1239 Size: 393 2003 597 787 268 1524 Fragments arranged by size: 2003 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2428 2428 Size: 5572 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 4719 1678 Size: 3041 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2295 2327 2488 2520 4213 Size: 32 135 32 723 32 161 32 1693 Cuts at: 4213 4245 4287 4319 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1693 723 161 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2806 2897 2806 Size: 91 5481 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4686 4870 1305 Size: 3381 184 2007 Fragments arranged by size: 3381 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3750 Fragments arranged by size: 3750 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3530 3889 4048 518 Size: 648 2364 359 159 2042 Fragments arranged by size: 2364 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2988 3665 4813 5058 1606 Size: 1382 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5020 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NheI G'CTAG_C Cuts at: 1052 2677 1052 Size: 1625 3947 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2930 4475 1132 Size: 97 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 2130 1348 Size: 782 4790 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 2860 1108 Size: 1752 3820 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 2428 Size: 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2691 2924 830 Size: 1861 233 3478 Fragments arranged by size: 3478 1861 233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 2555 3820 5532 2555 Size: 1265 1712 2595 Fragments arranged by size: 2595 1712 1265 SfcI C'TryA_G Cuts at: 826 1045 2687 2920 4055 4736 4927 826 Size: 219 1642 233 1135 681 191 1471 Fragments arranged by size: 1642 1471 1135 681 233 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2522 2834 3676 4577 4839 820 Size: 197 926 579 312 842 901 262 1553 Fragments arranged by size: 1553 926 901 842 579 312 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 3011 513 Size: 552 1946 3074 Fragments arranged by size: 3074 1946 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2122 3599 3616 3769 3954 4399 325 Size: 1255 542 1477 17 153 185 445 1498 Fragments arranged by size: 1498 1477 1255 542 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2553 2596 3818 Size: 274 80 33 51 1443 576 43 1222 Cuts at: 3818 5530 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1222 576 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2704 2736 5532 5564 2704 Size: 32 2796 32 2712 Fragments arranged by size: 2796 2712 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI